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Biomedical subjects

Sergio Kuttler

Publications and source records attributed to Sergio Kuttler.

8 recordsLinked to original sources
PubMed2006

Upregulation of basic fibroblast growth factor in human periapical lesions.

Basic fibroblast growth factor is one of a class of heparin-binding growth factors that stimulates endothelial cell proliferation and migration in vitro and angiogenesis in vivo. The purpose of this study was to investigate the expression of basic fibroblast growth factor in chronic periapical lesions of endodontic origin. Ten chronic inflammatory periapical lesions were examined using immunohistochemical staining. The experimental control group consisted of four specimens of uninflamed periodontal ligament tissue. Two independent observers graded the staining intensity for basic fibroblast growth factor. An immunopositive, cytoplasmic, and nuclear reaction for basic fibroblast growth factor, with varying degrees of upregulation was observed in all 10 chronic periapical lesions. We speculate that the formation of granulation tissue and the activation of epithelial cell rests in chronical apical lesions might be associated with a local rise in the tissue level of basic fibroblast growth factor. Therefore, this growth factor could play an important role in the pathogenesis of chronic apical periodontitis and periapical cysts.

Case-Control Studies↗
PubMed2004

The properties of Endocal 10 and its potential impact on the structural integrity of the root.

The purpose of this research was to determine if the use of Endocal 10 (previously called Biocalex 6.9) is associated with root fracture and to assess its sealability. Thirty-six freshly extracted, single canal human mandibular incisors were instrumented and randomly divided into two equal groups (n = 15). Canals in group A were obturated with vertically compacted gutta-percha and Sealapex, and those in group B were filled with Endocal 10 placed with a #25 Lentulo spiral per manufacturer's instructions. Two blinded investigators evaluated the teeth for fractures using transillumination and an operating microscope at 12 x magnification. Three of the fifteen samples filled with Endocal 10 had vertically fractured in half, whereas none of the teeth filled with gutta-percha had any evident fracture lines. The remaining teeth were tested for leakage via a fluid filtration model at 1 wk and 30 days postobturation. No leakage was found among any of the samples whether filled with gutta-percha or with Endocal 10 at either time interval. Statistical analyses were completed using Fisher's exact test (p = 0.023), which showed that there was a significant increase in chance of fracture when using Endocal 10 versus gutta-percha. The results indicate that, although Endocal 10 is able to seal the tooth as well as gutta-percha and sealer, there is a significant potential risk of root fracture.

Calcium Compounds↗
PubMed2004

The impact of post space preparation with Gates-Glidden drills on residual dentin thickness in distal roots of mandibular molars.

BACKGROUND: Posts frequently are used to retain buildups. The authors examined the effects of post space preparation with Gates-Glidden drills on residual dentin thickness in distal roots of mandibular molars. METHODS: The authors embedded 26 root-treated mandibular molars in endodontic cubes and sectioned them perpendicularly to their long axis at 1.8-millimeter intervals. They placed Gates-Glidden drills nos. 3, 4, 5 and 6 sequentially to 5 mm short of working length in the distal canals. After using each drill size, the authors measured the dentin thickness from light microscopic images of the tooth slices. RESULTS: The authors analyzed data to detect changes resulting from canal instrumentation. They performed statistical analysis by partitioning the variability in a nested analysis of variance. Strip perforations occurred with a no. 4 Gates-Glidden drill 7.3 percent of the time and more frequently with larger drills. After endodontic treatment alone, the canal wall on the furcal side was less than 1 mm thick 82 percent of the time and less than 0.5 mm thick 17.5 percent of the time. CONCLUSIONS: Dentin thickness correlates inversely to post space diameter. A no. 4 Gates-Glidden drill caused strip perforations in 7.3 percent of the canals studied, and therefore the authors recommend that Gates-Glidden drills larger than a no. 3 not be used in these roots. After endodontic treatment, the furcation-side dentin thickness was less than 1 mm in 82 percent of the teeth. CLINICAL IMPLICATIONS: Post space preparation in mandibular molars carries significant risk of perforation. Post space in such teeth should be limited to the endodontically prepared canal.

Dental Instruments↗
PubMed2003

An in vitro evaluation of the sealing ability of a new root-canal-obturation system.

In this study the sealing ability of a new urethane methacrylate resin-based sealer, EndoRez, was evaluated using a fluid-filtration model. Sixty-four single-rooted lower bicuspids were decoronated, instrumented, and divided into 3 groups of 20 each with 4 teeth used as controls. In group A, the roots were obturated with EndoRez and a single cone of gutta-percha, group B with AH Plus and a single cone of gutta-percha, and group C was obturated using gutta-percha with warm vertical compaction and AH Plus sealer. All specimens were allowed to set for 7 days in 100% humidity at 37 degrees C. The groups were compared for differences in the amount of leakage (mm/h) using a Chi-square test. The leakage of group A was significantly higher at p = 0.01 than the other two groups. There was no significant difference in leakage between groups B and C.

Bicuspid↗
PubMed2003

The crystallization of sodium hypochlorite on gutta-percha cones after the rapid-sterilization technique: an SEM study.

The purpose of this study was to identify the presence, crystallization, and subsequent removal of sodium hypochlorite crystals on gutta-percha cones after rapid sterilization. Seventy-two, fresh, standardized, gutta-percha cones were randomly selected. Each cone was observed under the scanning electron microscope and the elemental analysis machine before and after rapid sterilization using 5.25% and 2.5% sodium hypochlorite. Gutta-percha cones were rinsed after sterilization with 96% ethyl alcohol, 70% isopropyl alcohol, and distilled water independently to determine which of these agents removed the sodium-chloride crystals. There were no crystals present on the gutta-percha cones directly from the box. All gutta-percha cones had sodium-chloride crystals present after the rapid-sterilization technique using 5.25% and 2.5% sodium hypochlorite. However, the sodium-chloride crystals were removed by 96% ethyl alcohol, 70% isopropyl alcohol, and distilled water.

Alcohols↗
PubMed2002

Canal-centering ability of two rotary file systems.

The ability of two nickel-titanium rotary file systems to maintain the original path of the canal were compared by using a new split-mold design (the Endodontic Cube). The mesial roots of 10 mandibular first- and second-molar teeth with separate canals from orifice to foramen were divided into two groups based on canal curvature and morphology. Working lengths for each canal were determined 1.0 mm short of where the file tip was visible on the external surface of the root. The teeth were embedded in composite resin by using the Endodontic Cube as a mold and sectioned into five pieces. ProFile Ni-Ti.06 taper Series 29 and ProFile GT rotary instrumentation techniques were randomly assigned to one of the canals and used in a crown-down fashion to a 0.217 ProFile and a 0.06 GT master apical file, respectively. Digital photographs were made of the middle three sections and software features of Adobe Photoshop were used to measure the movement of the canal centers by superimposing the images of the instrumented and uninstrumented canals. Data were analyzed using ANOVA. Both systems remained centered within the canal with minimal deviation from the original canal path. The largest mean movement was less than 0.15 mm. The Endodontic Cube was an effective tool for studying and comparing instrumentation techniques.

Analysis of Variance↗
PubMed2002

Effect of electronic apex locators on cardiac pacemaker function.

The purpose of this study was to assess the effects of five electronic apex locators on pacemaker function in vitro. A Biotronik Actros DR+ pacemaker was evaluated at maximum sensitivity on a flat bench top. The pacemaker lead, electronic apex locator, and oscilloscope were connected across a 150-ohm resistor. Pace monitoring was carried out with a Biotronik EPR 1000 programmer and a Tektronix TDS 220 2-channel digital real-time oscilloscope. Four of five electronic apex locators tested did not cause inhibition or interfere with normal pacemaker function. It seems that electronic apex locators can be used safely in patients with pacemakers.

Dental Instruments↗