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Seung Won Kim

Publications and source records attributed to Seung Won Kim.

2 recordsLinked to original sources

Mist generation from metalworking fluids formulated using vegetable oils.

Metalworking fluid emulsion formulations produced from vegetable oils may be less toxic and may reduce disposal costs when compared with fluids formulated with petroleum-based oils. Experiments were performed on experimental emulsions made with unmodified and modified soybean oils to measure rates of mist production by impaction, centrifugal force and evaporation/recondensation mechanisms. Results were compared with measurements made using a commercial metalworking fluid emulsion formulated using vegetable oil and another made from mineral oil. The results indicated that most of the experimental fluids produced about the same amount of mist as the commercial fluids by impaction and more mist than the petroleum-based fluid by centrifugal force. However, an air-oxidized modified soy oil produced less mist by impaction than the petroleum-based fluid and about the same by centrifugal force. The experimental fluids produced between 30 and 90% less mist than the commercial fluids by evaporation/recondensation. The air-oxidized soybean oil was the most promising candidate among the experimental fluids for further testing in more realistic machining conditions.

Air Pollutants, Occupational↗

Mechanism of silica-induced ROS generation in Rat2 fibroblast cells.

Reactive oxygen species (ROS) play an important role in cell signaling pathway. Previously, we found that silica induced immediate ROS generation and sequential cellular responses such as kinase activation in Rat2 cells as well as an increase of intracellular calcium concentration in A549 cells. However, the detailed mechanism underlying the immediate ROS generation induced by silica in fibroblast cells remains to be elucidated. Therefore, in the present study, we investigated the mechanism of ROS generation by silica within Rat2 fibroblast cells by examining the effects of a diverse group of inhibitors for the enzymes related with signal transduction events. Inhibitors for protein tyrosine kinase (PTK), phospholipase C (PLC), protein kinase C (PKC) and calmodulin (CaM) kinase II effectively suppressed ROS generation in silica-stimulated Rat2 cells, whereas those for protein kinase A and phospholipase A(2) did not. Diphenyleneiodonium chloride (DPI), an inhibitor for NADPH oxidase was also found to be effective in inhibiting silica-induced ROS generation. These results suggest that PTK, PLC, PKC, CaM kinase II, and NADPH oxidase are all involved in signal transduction pathways for ROS generation in silica-stimulated Rat2 cells.

Animals↗