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Shakila Zaman

Publications and source records attributed to Shakila Zaman.

6 recordsLinked to original sources
PubMed2006

Cytokines in the placenta of Pakistani newborns with and without intrauterine growth retardation.

Although intrauterine growth retardation (IUGR) is a major risk factor for increased neonatal mortality and morbidity, the mechanisms behind it are not clear. We analyzed cytokine gene expression and gene polymorphisms in infants with and without IUGR in Pakistan, where IUGR is very common. 45 IUGR and 55 control mother/infant pairs were studied. mRNA for IL-10, IL-8, TNF-alpha, TGF-beta, IL-6, IL-4, IL-1beta, IL-12, IFN-gamma and GAPDH was quantified with RT-PCR from placenta. Cytokine and cytokine receptor gene polymorphisms for -1087IL10, -308TNFA, -174IL6, +915TGFB1, intron 2 IL1RN, +36TNFR1, 150V IL4RA and -159CD14 were determined from genomic DNA. The serum levels of IL-1beta, IL-6, IL-8, IL-10, IL-12, TNF-alpha and TGF-beta were measured. There was a significant decrease of IL-10 and IL-12, but increase of TGF-beta in the decidua and similarly decrease of IL-10, but increase of TGF-beta in the trophoblasts of the IUGR placentas compared with the non-IUGR placentas. We found significantly lower levels of IL-1beta in serum from the mothers of the IUGR infants and of TGF-beta in serum of the infants with IUGR compared with the non-IUGR infants. We note that the IL-10 mRNA expression in the decidua was down-regulated, but the TGF-beta mRNA up-regulated in IUGR placentas of mothers from a population with multiple risk factors for IUGR. We propose that the low IL-10 in the placenta may be involved in the pathogenesis of IUGR and might possibly be treatable.

Base Sequence↗
PubMed2005

Folate, vitamin B12, and homocysteine levels in South Asian women with growth-retarded fetuses.

OBJECTIVE: To investigate whether intrauterine growth retardation (IUGR) and preterm delivery in a poor population of South Asia was associated with altered maternal and fetal levels of folate, vitamin B12, and homocysteine. SUBJECTS AND METHODS: Hundred and twenty-eight pregnant women from a low socio-economic strata in the city of Lahore, Pakistan were followed with ultrasound of fetal growth from the 12th week of pregnancy. Blood samples were drawn from the woman and the cord at delivery. Serum was analyzed by a chemiluminescent immunoassay for folate and vitamin B12 and by fluorescence polarization immunoassay for total homocysteine (tHcy). RESULTS: Fourty-six infants showed IUGR. In term, but not preterm, deliveries with IUGR, maternal and cord blood folate levels were half of those in deliveries of normal birth weight infants (P=0.004 and P=0.005). The risk of IUGR was reduced among women with folate levels in the highest quartile (OR 0.31, 95% CI 0.10--0.84). There was no association between vitamin B12 and IUGR. Total homocysteine levels were higher in women delivering IUGR infants (P=0.02). There was an inverse correlation between cord blood folate and tHcy levels (r=-0.26, P=0.006). We also found increased risks for hypertensive illness (OR 3.5, 95% CI 1.4--8.6) and premature delivery (OR 2.5, 95% CI 1.1--6.2) in women in the highest quartile of tHcy. CONCLUSIONS: The occurrence of IUGR increased with low maternal and cord concentrations of folate and high maternal levels of tHcy. Further studies on the effects of vitamin B supplementation through pregnancy are warranted.

Adult↗
PubMedDate not supplied

Childhood stunting in relation to adolescent living environment and resilience: a follow up study in Lahore, Pakistan.

BACKGROUND: Stunting, as a manifestation of deprivation in early childhood, is a common problem among young Pakistani children. Poverty and lack of resources may predispose a child to maladjustment in the grown-up society. Resilience has been studied in young children to ascertain how the children cope with the challenges in life. The aim of the investigation was to study differences in growth, socio-economic situation and resilience between adolescent boys who had been stunted or normal in height at the age of five years. METHODS: Using both quantitative and qualitative study designs, the study areas were an urban slum area and a village outside Lahore, Pakistan. All boys (n = 36) had been followed from birth to 5 years of age in an earlier epidemiological study conducted at the Dept of Social and Preventive Paediatrics, King Edward Medical College, Lahore and were identified for follow up at 12-15 years of age. RESULTS: Those who were stunted at 5 years were also shorter as adolescents than those who were normal in height at 5 years of age. Resilience, i.e., the combination of the adolescent's emotional abilities, his access to emotional and family support and view of himself was interestingly not heavily influenced by earlier malnutrition. CONCLUSIONS: Despite the hardships faced by the young adolescent boys, living in poor socio-economic situations, are capable of displaying resilience despite being stunted.

Adaptation, Psychological↗