Biomedical subjects

Shang-Zhi Huang

Publications and source records attributed to Shang-Zhi Huang.

5 recordsLinked to original sources

PubMed2006

Erectile dysfunction in Fragile X patients.

AIM: To report the clinical experience during collecting sperm samples in the Fragile X syndrome (FXS) male patients. METHODS: Two different polymerase chain reaction (PCR) based methods were used for the molecular diagnosis of FXS. Sperm collection was done mostly according to the laboratory manual of the World Health Organization. RESULTS: We failed to collect sperm samples from five Fragile X subjects aged 18-60 years as a result of an unexpected erectile dysfunction (ED). Multiple examinations of the same subject at different times, and of different subjects from different provinces examined by different physicians, showed the same result consistently in all the five subjects. CONCLUSION: Erectile reflex is an instinctive response in all healthy males. The absence of erection can be caused by hormonal, physical or neuronal malfunction. As hormonal profiles were reported to be generally normal in Fragile X men, we propose that an unknown physical factor or the neuronal circuit, or both, underlying the erection is compromised. The finding of this symptom in Fragile X patients may help better understand the clinical spectrum and pathogeneses of the disease.

Adolescent↗

PubMed2005

[The cDNA cloning of conarachin gene and its expression in developing peanut seeds].

In order to clone the genes of peanut seed storage proteins, a cDNA library of mid-maturation stage cotyledons of peanut (Arachis hypogaea L. Shanyou 523) was constructed. After immunoscreening with polyclonal antibody against conarachin from peanut seeds, two cDNA sequences named Ahy alpha and Ahy beta of conarachin genes were obtained. BLAST these sequences in GenBank showed that they share high homology with the genes of legume 7S globulins. One of the sequences (Ahy beta) was found to be identical to part of the published complete cDNA sequence of the peanut allergen, Ara h1 p17. The other fragment (Ahy alpha) was almost identical to parts of the published complete cDNA sequence of the peanut allergen, Ara h1 p41b. Results of southern analysis show that they belong to a small gene family (the result is not shown). These sequences were expressed only in developing peanut seeds. To isolate the genes involved in seed storage protein and to understand the gene expression during peanut seed development, the sequences of 414 ESTs were determined and further analyzed by BLAST searches and categorized functionally. Five rESTs (representative ones) of arahin, two rESTs of conarachin and six rESTs of conglutin-like proteins were isolated. 17.0% of total transcripts isolated in this study are involved in peanut seed storage proteins.

Allergens↗

PubMed2004

ING4 induces G2/M cell cycle arrest and enhances the chemosensitivity to DNA-damage agents in HepG2 cells.

The known members of inhibitor of growth (ING) gene family are considered as candidate tumor suppressor genes. ING4, a novel member of ING family, is recently reported to interact with tumor suppressor p53, p300 (a major component of histone acetyl transferase complexes), and p65(RelA) subunit of NF-kappaB. In this study, we investigated the cellular behaviors of HepG2 cells with exogenous ING4. Interestingly, the overexpression of ING4 negatively regulated the cell growth with significant G2/M arrest of cell cycle, and moreover, enhanced the cell apoptosis triggered by serum starvation in HepG2 cells. Furthermore, the exogenous ING4 could upregulate endogenous p21 and Bax in HepG2 cells, not in p53-deficient Saos-2 cells, suggesting that G2/M arrest induced by ING4 could be mediated by the increased p21 expression in a p53-dependent manner, although there is no significant increase of p53 expression in HepG2 cells. Moreover, HepG2 cells with exogenous ING4 could significantly increase cell death, as exposed to some DNA-damage agents, such as etoposide and doxorubicin, implying that ING4 could enhance chemosensitivity to certain DNA-damage agents in HepG2 cells.

Acetyltransferases↗

PubMed2004

[Synthesis of heat shock proteins in developing maize embryo].

Labeling of protein in developing maize embryo with (35)S-Met showed that, the protein synthesis rate in heat shock (42 degrees C) was similar to that in the control (25 degrees C). Heat shock inhibited protein synthesis at 16 DAP, whereas it stimulated protein synthesis at 22 and 34 DAP. The pattern of SDS-PAGE showed that, a new set of heat shock proteins were induced after heat shock. At 16 DAP, heat shock induced 3 kinds of HSPs with molecular weight 86.4, 80.0 and 73.2 kD. And at 22 DAP, 7 kinds HSPs were induced with molecular weight 86.4, 80.0, 73.2, 24.4, 18.2, 16.8 and 13.6 kD. After 22 DAP, except 3 kinds of protein with molecular weight 73.2, 24.4 and 18.2 kD were induced turnover synthesis, other 4 kinds were increased by heat shock. The pattern of 2D-PAGE further suggested that, heat shock induced more than 20 kinds of HSPs at 22 and 28 DAP, especially the smHSP of 20-30 kD were more than 10 kinds. Western blot indicated that, BiP (HSP70) and PDI (HSP60) kept a high expression level during the embryo development, and failed to respond to heat shock.

Heat-Shock Proteins↗

PubMed2004

[Induction of chilling tolerance and heat shock protein synthesis in rice seedlings by heat shock].

Heat shock applied to germinated rice seeds increased the chilling tolerance of seedlings. Comparison with the control, brief heat shock applied before chilling at 4 degrees C for 2 days and recovery at 25 degrees C for another 2 days decreased the permeability of cellular membranes and increased the content of proline in rice seedlings. Heat shock applied before chilling also increased the activities of superoxide dismutase, catalase, and peroxidase and the content of ascorbate in rice seedlings. In contrast, the lipoxygenase activity and the malondialdehyde content in the heat-shocked rice seedlings were lower than those in the control. Germinated rice embryos synthesized heat shock proteins of Mr 78, 70, 64, 46, 38, 24, 17 and 16 kD during heat shock. The results of Western blot suggested that the binding protein (Bip) of HSP70 play an important role in protecting the rice seedling against chilling damage.

Arabidopsis Proteins↗