Biomedical subjects

Sheng-Bin Li

Publications and source records attributed to Sheng-Bin Li.

12 recordsLinked to original sources

PubMed2006

[Genetic polymorphisms of 5 X-STR loci in Yunnan Nu population].

OBJECTIVE: To investigate the allele and genotype frequencies of DXS6804, DXS6799, DXS8378, DXS7130 and DXS7132 in unrelated individuals of Nu population and establish the related genetic database. METHODS: Five X-STR loci were analyzed by PCR followed PAGE and silver staining. RESULTS: The allele frequencies of the five X-STRs in Yunnan Nu population are in accordance with Hardy-Weinberg equilibrium. CONCLUSION: Five X-STRs loci of Nu population could be used in forensic identification.

Alleles↗

PubMed2005

Alterations of serum cholinesterase in patients with gastric cancer.

AIM: To understand the correlation of serum cholinesterase (CHE) activity with gastric cancer and to assess their clinical significance. METHODS: The velocity method was adopted to detect the activity of serum CHE in patients with gastric cancer and in patients with non-malignant tumor as controls. RESULTS: The serum CHE activity in the treatment group was significantly lower than that in the control group with a very significant difference between the two groups (83.3:113.1,P = 0.0003). Age was significantly associated with the incidence of gastric cancer. CONCLUSION: Serum CHE activity has a close relation with the incidence of gastric cancer.

Adult↗

PubMed2004

[Sequencing and analyzing of expressed sequence tags (ESTs) of pig fat tissue].

In this paper,the ESTs of pig fat tissue were sequenced and analyzed using the large-scale DNA sequencing method, 7790 high quality ESTs were gained,from which 4354 genes were obtained using cluster analysis with the STACK-PACK software, including 3609 singlet genes and 745 multicopy genes. The 4354 candidate genes were compared with BLASTN to the nr library (e =1e-10), of which 2712 were known genes, containing 1 987 singlet genes and 725 multicopy genes,there are 2 109 unknown genes and new ESTs. Based on the results of BlastN and the index of GenBank Accenssion No., the known gene expression profile of pig fat were constructed. It showed that the genes participating in metabolism held the highest proportion in the 7 sorts, and in some aspects showed the hearty metabolism activity of fat tissue. 257 total reads of the Major Histocompatibility Complex (MHC) genes in the pig fat tissue were found,including 181 singlet genes and 44 multicopy genes. They account for 44.9% of the cell and organism defense, and 5.4% of all known genes,from this we can see that the MHC has a fairly high expression degree. Comparing all ESTs related with MHC (257reads) with known pig BAC we found the partial sequences (approximately 200 bp) of the ESTs distributing to every exon of. We can predict that the ESTs in MHC contain approximately 200 bp highly consistent regions and every exon possesses one of these regions. Although these MHC sequences in different BAC have different protein domains,they are highly consistent and related with immune functions. When the MHC genes transfer to the next generation,they can duplicate repeatly and inherit stably.

Adipose Tissue↗

PubMed2003

Fabrication and optimization of HLA-DRB1-12 oligonucleotide microarray.

Oligonucleotide microarray is developed on the basis of hybridization on the solid substrate. The pre-activated glass substrates and the terminal modification of the oligonucleotides are the two important factors in the process of fabrication for microarray. In order to compare the hybridization signal intensity of the different terminal modified oligonucleotide probes, the eight kinds of oligonucleotides were designed according to the sequence of HLA-DRB1-12, including the amino modified oligonucleotides with PEG spacer and the one without spacer, the phosphorothioate modified oligonucleotides with PEG spacer and the one without spacer. They were modified on 5' terminal and 3' terminal, respectively. In addition, the oligonucleotides probes with the internal spacer of different number of PEG were designed to observe the relationship between the spacer of PEG and the hybridization efficiency. These probes were respectively fixed on the bromoacetylation activated and glutaraldehyde activated slides to manufacture the two kinds of microarray which hybridized with the fluorescence labeled PCR product of HLA-DRB1-12 gene. The results from the study demonstrated that the signal intensity of 3' amino-modified probes with the internal spacer of different number of PEG on the bromoacetylation activated slides was stronger than the others. It is concluded that the 3' amino-modified oligonucleotide with an internal PEG spacer and the bromoacetylation activated slide enhanced the hybridization efficiency and were worthy to be proposed for the fabrication of HLA microarray or other kinds of microarrays for detecting fluorescence labeled PCR product in the future study.

HLA-DR Antigens↗

PubMed2003

[The study of HLA-Cw polymorphism in Uygur population].

The HLA-Cw loci polymorphism in Uygur population was investigated using the PCR- sequence specific oligonucleotide probe (SSOP) method,and the genetic database on the distribution of gene frequency of the HLA-Cw loci was established. From 146 individuals of Uygur population,18 HLA-Cw alleles were detected. The gene frequency was from 0.0069 to 0.2460. The four most common alleles were HLA-Cw*04(24.60%),07(11.51%),08(10 10%),14(12.02%),and they covered 58.23% of total alleles detected from Uygur population.We have made a survey of HLA-Cw alleles frequencies in a Uygur population,with blank frequency being lowered to 0.0064. The distribution of genotype frequencies met the law of Hardy-Weinberg equilibrium by hi-square test. The frequency data can be used in forensic and paternity tests to estimate the frequency of a DNA profile in the Uygur population,transplant matching and anthropology.

English Abstract↗

PubMed2002

Analysis of DNA polymorphism at HLA-A locus by PCR amplification with sequence specific oligonucleotide probe in Chinese Han and Uygur populations.

To determine HLA-A genetic polymorphism in Chinese populations and establish ethnic genetic database, 165 Han and 162 Uygur subjects were investigated with a non-isotopic and sensitive method PCR-SSOP. 22 alleles were identified in Han with the most frequent allele being HLA-A * 1101 (19.7%), followed by * 0201 (12.72%). Also, 22 alleles were identified in Uygur with * 2407 (17.90%) being the most frequent one and the frequencies of following alleles: * 0201, * 0101, * 3301 were higher than 10%. HLA-A * 0203, * 0205, * 0210, * 0302, * 2403 and * 3302 were only detected in Han; meanwhile * 0205, * 0211, * 2301, * 2502, * 68012 and * 6802 were only in Uygur. According to Hardy-Weinberg equilibrium, each allele showed no significant (P > 0.05) deviation between the expected frequency and the observed one. Heterozygosity (H), discrimination power (DP) and probability of paternity exclusion (EPP) of HLA-A locus from Han nationality were computed to be 0.9029, 0.9776 and 0.8592; and those from Uygur as 0.9063, 0.9379 and 0.7885. These results suggest that HLA-A DNA polymorphism and the database of two Chinese populations have useful applications in processing forensic casework (as personal identification, paternity test), tracing population migration and genetic diagnosis.

Alleles↗

PubMed2002

[Genetic structure of STR in Naci ethnic group in China].

STR is a universal genetic marker that has changeable polymorphism and stable heredity in human genome. It is a specific DNA segment composed of 2-7 base pairs as its core sequence, and is formed through the repeated connection of the same one. Since it has the characteristics such as numerous allelic genes, highly heterozygosity and easy recognition and short PCR segment, it is employed as an ideal DNA marker in such practical fields as human genetics and forensic medicine. In this study, we investigated the polymorphism of STR of Naci minority with STR genescan marked by fluorescence. Seventy-two alleles of 9 STR in Naci were detected with their frequency 0.0052-0.5208 and 165 genotypes were found out with frequency 0.0104-0.3021. Hi-Square test indicated the distribution of genotypes agreed with Hardy-Weinberg equilibrium (P > 0.05). Statistical analysis showed the followings: the heterozygosity (H) > 0.6 in each locus, the average polymorphism information content (PIC) > 0.7, Mean discrimination power (DP) > 0.8, probability of paternity exclusion (EPP) > 0.5, indicating that the STR markers used in the study were of great value in the researches of minority genetics. This not only founds the base for genetic structures of STR of Chinese but also provides valuable information for anthropology, forensic medicine and ethnology.

China↗

PubMed2002

[STR polymorphisms in four Xinjiang ethnic groups in China].

Population genetic studies were performed in Xinjiang's Uygur, Sibe, Ozbek and Kirgiz. Allele frequency distributions were analyzed for ten loci, i.e., D3S1358, VWA, CSF1PO, FGA, THO1, TPOX, D5S818, D13S317 and D7S820 by GeneScan. The results showed that there were 66 STR alleles and 148 genotypes in Uygur; 72 STR alleles and 163 genotypes in Sibe; 65 STR alleles and 168 genotypes in Ozbek; 71 STR alleles and 191 genotypes in Kirgiz. Significant differences were identified among ethnic groups (African-American, US-Caucasian and Chinese-Oriental), but similarity was found among the four Xinjiang population. These findings indicated that the nine STR loci and Amelogenin locus were very useful for individual identification in forensic science. It is further certified that Chinese nationality is a whole.

Alleles↗

PubMed2002

[Studies of STR polymorphisms in Pumi and Lisu minorities in China].

In this study, we investigated the polymorphisms of STR of Pumi and Lisu minorities with STR genescan marked by fluorescence. Eighty-five alleles of 9 STR in Pumi were detected with the frequency 0.0050-0.5250 and 194 genotypes were found with frequency of 0.0098-0.3235. Sixty three alleles with their frequency of 0.0050-0.4802 and 145 genotypes were found out with frequency of 0.0099-0.3664 in Lisu population. Hi-Square test indicated the distribution of genotypes agreed with Hardy-Weinberg equilibrium (P > 0.05). Statistical analysis showed the followings: H > 0.6 in each locus, the average PIC > 0.7, mean DP > 0.8, EPP > 0.5, indicating the STR markers used in the study were of great value in the researches on minority genetics.

Alleles↗

PubMed2002

[Genetic polymorphism of 9 STR loci in Zhuang national minority of China].

Genetic distribution for nine STR loci were determined in a Chinese Guangxi Zhuang national minority group based on STR gene scan marked by fluorescence. Sixty-two alleles and 169 genotypes were observed in 91 unrelated Zhuang individuals. The corresponding gene frequency and genotype frequency were 0.0054-0.5495 and 0.0110-0.3297 respectively. The expected and observed genotype frequency of nine STR loci was in accordance with the Hardy-Weinberg equilibrium (P > 0.05). The statistical analysis of nine STR loci showed PIC (polymorphic information content) > or = 0.6088, H (heterozygosity) > or = 0.6174, DP (discrimination power) > or = 0.8028, PPE (probability of paternity exclusion) > or = 0.8165. The genetic distance figured with 9 STR genetic data showed that there were significant differences between Chinese Zhuang national minority and the American White and the American Black, and there was little difference between Zhuang national minority and the Chinese Xi'an Han. The result of clusting showed that the present data were divided into three groups: the Black, the White and the Yellow (the Chinese).

Alleles↗

PubMed2002

[Polymorphism of nine STR locus in Nu population from Yunnan Province].

In this study,blood samples were randomly drawn from 84 unrelated Nu individuals. The polymorphism of nine STR loci and Amelogenin locus were determined by DNA GeneScan. The genetic database on the distribution of gene frequency on the nine STR loci was established, statistical results showed that the genotype distributions were in agreement with Hardy-Weinberg equation. Compared with other population,the results in our study were of great value in human DNA genetic data instant method with the characteristics of precision and sensitivity.

English Abstract↗

PubMed2002

[Genetic polymorphisms of 9 STR loci in Dongxiang ethnic group of China].

Genetic distribution for nine STR loci was determined in a Chinese Dongxing ethnic group based on STR genescan marked by fluorescence. Seventy-two alleles and 182 genotypes were observed in 94 unrelated Chinese Dongxiang individuals,with the corresponding gene frequency and genotype frequency being 0.0053-0.5825 and 0.0106-0.2660 respectively. The genotypes of nine STR loci were in accordance with the Hardy-Weinberg equilibrium (P>0.05). The statistical analysis of nine STR loci showed PIC (polymorphism information content, PIC) = or > 0.6378, H(heterozygosity, H) = or > 0.6500, DP (discrimination power, DP) = or > 0.8216, PPE (probabilities of paternity exculation, PPE) = or > 0.4903. The result indicated that there was a significant difference between Dongxiang ethnic group and the white and the black. There was no significant difference in Han nationality. These result filled the Dongxiang ethnic group-a specific group of Chinese into the genetic database and played an important role in Chinese population genetic study and forensic medicine application.

English Abstract↗