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Biomedical subjects

Shi-Li Wang

Publications and source records attributed to Shi-Li Wang.

5 recordsLinked to original sources
PubMed2006

Study of recombinant human osteogenic protein-1 expressed in prokaryocyte on the repair of extracted socket in rabbits.

The study investigated the effect of recombinant human osteogenic protein-1 (rhOP-1) expressed in prokaryocyte, to promote the healing of alveolar socket. A model of rabbit extracted socket into which the composites of rhOP-1 and gelatin sponge was immediately implanted was created and the osteoinduction of rhOP-1 was assessed by histological method, quantitative measurement of calcium content and alkaline phosphatase (ALP) activity. The result of histology showed that bone healing in rhOP-1 side is 4-6 weeks earlier than that of the control side. ALP activity and calcium content in rhOP-1 side were significantly high compared with that of the control side. rhOP-1 has a satisfactory osteoinduction ability to promote the healing of extracted socket.

Alkaline Phosphatase↗
PubMed2005

Feature characterization of microfabricated microfluidic chips by PDMS replication and CCD imaging.

This paper presents a new approach for the metrological characterization of microfabricated features on microfluidic chips, based on a combination of poly(dimethylsiloxane) (PDMS) replication and charge-coupled device (CCD) imaging. A PDMS replicate was cast from the original chip sample, and a 2-mm thick sample slice was cut from the replica at the cross-section to be studied. The digital image of the revealed structural profile was captured by a CCD camera under a microscope, and the image was processed using specially-developed algorithms for CCD image calibration and edge detection. Depth and width measurements obtained using the method agreed well with those gained using a stylus profiler and universal measuring microscope, with a deviation of below 0.9 mum, while profile distortions of deeper structures using stylus profilers were avoided. The method is reliable, non-destructive, and cheap and simple to implement in any analytical laboratory.

Journal Article↗
PubMed2005

A simple microfluidic system for efficient capillary electrophoretic separation and sensitive fluorimetric detection of DNA fragments using light-emitting diode and liquid-core waveguide techniques.

A miniaturized CE system has been developed for fast DNA separations with sensitive fluorimetric detection using a rectangle type light-emitting diode (LED). High sensitivity was achieved by combining liquid-core waveguide (LCW) and lock-in amplification techniques. A Teflon AF-coated silica capillary on a compact 6x3 cm baseplate served as both the separation channel for CE separation and as an LCW for light transmission of fluorescence emission to the detector. An electronically modulated LED illuminated transversely through a 0.2 mm aperture, the detection point on the LCW capillary without focusing, and fluorescence light was transmitted to the capillary outlet. To simplify the optics and enhance collection of light from the capillary outlet, an outlet reservoir was designed, with a light transmission window, positioned directly in front of a photomultiplier tube (PMT), separated only by a high pass filter. Automated sample introduction was achieved using a sequential injection system through a split-flow interface that allowed effective release of gas bubbles. In the separation of a phiX174 HaeIII DNA digest sample, using ethidium bromide as labeling dye, all 11 fragments of the sample were effectively resolved in 400 s, with an S/N ratio comparable to that of a CE system with more sophisticated LIF.

DNA↗
PubMed2005

[Determination of sulfur dioxide in air by sequential injection spectrophotometry].

A sequential injection spectophotometric method for the determination of sulfur dioxide by decolorizing malachite green in an alkali medium is studied; the decreasing in color is proportional to the amount of sulfur dioxide. The loading sequence, the concentration of reagents, the amount of sample and the flow of reagent rate are optimized by univariate method. With sequential injection sample introducing, the volumes of sample and reagent are reduced greatly and the speed of determination is increased apparently, a throughput of 60 samples/h can be achieved. A liner calibration curve was obtained in range of 0.5-4.0 microg x mL(-1). The determination limit is 0.15 microg x mL(-1), the RSD and the recovery are 1.5% and 94%-108% respectively.

Air↗