Biomedical subjects

Shi-Ping Wang

Publications and source records attributed to Shi-Ping Wang.

6 recordsLinked to original sources

PubMed2006

Eosinophilic granuloma of the jaws: an analysis of clinical and radiographic presentation.

Eosinophilic granuloma (EG) is the localized and mildest form of histiocytosis-X group of diseases. In this study, 22 patients of pathologically proven EG involving the jaws were retrospectively studied concerning the clinical and radiographic presentation. The clinical and radiographic presentation of the 22 cases was quite varied, and the accuracy of clinical and radiographic diagnosis was low. However, the clinical and radiographic presentation correlated with the lesion site, and accordingly all the jaw lesions could be divided into three types (Alveolar Type, Intraosseous Type, and Mixed Type). Our classification based on the lesion sites reveals the correlation between the clinical and radiographic presentation of EG and the lesion sites, and may be useful in the identification of lesions of EG in the jaws.

Adolescent↗

PubMed2005

Silver-enhanced colloidal gold metalloimmunoassay for Schistosoma japonicum antibody detection.

A silver-enhanced colloidal gold metalloimmunoassay has been proposed for the determination of Schistosoma japonicum antibody (SjAb) in rabbit serum. The adult worm antigen of S. japonicum (SjAg) was adsorbed passively on the walls of a polystyrene microwell and then reacted with the desired SjAb. The colloidal gold-labeled goat anti-rabbit IgG secondary antibody was adsorbed on the walls of the polystyrene microwells through the reaction with SjAb, followed by the silver enhancement process, dissolution of silver metal atoms in an acidic solution, and determination of dissolved silver ions by anodic stripping voltammetry (ASV) at a glassy-carbon electrode. Assay conditions were optimized, including the reaction time of SjAg with SjAb, the interaction of SjAb with the colloidal gold-labeled secondary antibody, the dilution ratio of the colloidal gold-labeled secondary antibody and the silver enhancement time. The integration of the anodic stripping peak current depended linearly on the SjAb logarithmic concentration over the range of 6.4 ng/ml to 100 microg/ml. A detection limit as low as 3.0 ng/ml SjAb was achieved, which was better than the piezoelectric body acoustic wave sensor (detection limit of 7.2 microg/ml) and the renewable amperometric immunosensor (detection limit of 0.36 microg/ml). Rabbit serum samples with various degrees of infection were analyzed, and the results demonstrate that the proposed method meets the requirements of clinical analysis.

Animals↗

PubMed2003

Quartz-crystal microbalance immunosensor for Schistsoma-japonicum-infected rabbit serum.

A quartz-crystal microbalance immunosensor (QCM) has been developed for the direct determination of Schistosoma-japonicum-infected rabbit serum. A self-assembled monolayer with carboxyl groups was first coated on a gold electrode of a quartz-crystal resonator by the spontaneous adsorption of 3-mercaptopropionic acid. Schistosoma-japonicum molecular antigen of 32 kD molecular weight was then covalently attached to the crystal surface. The QCM immunosensor was used to detect infected rabbit serum (IRS49-2000); a maximum titer of 1:800 was achieved.

3-Mercaptopropionic Acid↗

PubMed2003

[A new method for EST clustering].

We developed an EST (expressed sequence tag) clustering method, ESTClustering, to generate high-quality unique expressed sequence based on large-scale EST sequencing. The method uses consensus sequences to sequence analyze with megablast and assemble each cluster with phrap in clustering process. The clustering strategy can efficiently identify gene family and alternate splicing forms of expressed sequences. It can also reduce the adverse effects caused by sequence errors. The ESTClustering method tends to provide more expressed gene forms comparing with the UniGene clustering method of the National Center for Biotechnology Information. Analysis of the 112,256 ESTs of Arabidopsis with ESTClustering produced 23,581 EST clusters. Among these Arabidopsis EST clusters, 13,597 have corresponding genome coding sequences and this number is close to the number of genes predicted with Arabidopsis ESTs. Using this clustering method, a total of 147,191 rice ESTs were clustered into 33,896 groups.

Algorithms↗

PubMed2002

Treatment of odontogenic keratocysts: a follow-up of 255 Chinese patients.

OBJECTIVE: The purpose of this study was to report our experience in the surgical treatment of 484 Chinese patients with follow-up in 255 cases. METHODS: A retrospective analysis was conducted of all odontogenic keratocysts that were surgically treated and histopathologically diagnosed between 1962 and 1998. The odontogenic keratocysts were surgically treated with enucleation, marsupialization followed by secondary enucleation, and resection with or without continuity defects. The recurrence rate was evaluated for the patients followed more than 3 years. RESULTS: Of a total of 489 odontogenic keratocysts, 327 (66.87%) were found in the mandible and 162 (33.13%) in the maxilla. Follow-up data were obtained in 255 patients. Recurrence was found in 31 cases (15.27%) in 203 patients treated with enucleation after an observation period of 3 to 29 years. Twenty-nine recurrences (17.79%) were found in 163 patients treated with enucleation alone, two recurrences (6.70%) were seen in 29 patients who received Carnoy's solution fixation of the cyst wall before enucleation, and no recurrence was found in 11 cases treated with marsupialization in combination with secondary enucleation. No recurrence was seen in 52 patients treated with resection. CONCLUSION: Odontogenic keratocysts treated with enucleation alone have a higher recurrence rate. Enucleation with adjunctive treatment can decrease recurrence rate. Radical excision has no recurrence but does have the highest morbidity rate and should be reserved for multiple recurrent cysts after conservative means.

Acetic Acid↗

PubMed2002

[Analysis and mapping of homologous sequences of barley disease resistance gene Mlo and maize disease resistance gene Hm1 in rice].

The encoding products of the disease resistance (R) gene of barley, Mlo, and the maize R gene, Hm1, do not contain the recognizable structural domains of most of the known plant R gene products. The two genes do not comply with the gene-for-gene theory in the response to pathogen infection either. Two sequences, OsMlo-1 and DFR-1 which were homologous to the barley Mlo and maize Hm1, respectively, were isolated from rice. The OsMlo-1 was mapped between two molecular markers, RZ667 and RG424, and located at a distance of 20.6 centi-Morgan (cM) from RZ667 and 6.0 cM from RG424 on rice chromosome 6. The DFR-1 was flanked by molecular markers R2635 and RG462 with an estimated genetic distance of 11.3 cM from R2635 and 23.9 cM from RG462 on rice chromosome 1. Using other published rice molecular linkage maps as references, it was found that the chromosomal locations of OsMlo-1 and DFR-1 were correspondence with two previously identified rice blast resistant QTLs (quantitative trait loci), respectively. The results suggest that the genes homologous to barley Mlo and maize Hm1 in rice may be involved in defense responses induced by pathogen infection.

Chromosome Mapping↗