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Biomedical subjects

Shigekuni Okisaka

Publications and source records attributed to Shigekuni Okisaka.

At least 19 recordsLinked to original sources
PubMed2006

[Repetitive instillation of 0.1% cyclosporin A eye drops induces miosis and anterior chamber inflammation-like reactions in monkey eyes].

PURPOSE: Miosis and anterior chamber inflammation-like reactions were recognized after six instillations of 0.1% cyclosporin A eye drops every 30 minutes into rabbit conjunctival sacs. In order to consider species specificity, 0.1% cyclosporin A eye drops were applied by the same method in monkeys. METHODS: Eye drops were applied in five monkeys (monkey A, B, C, D, E); in one eye as control and in nine eyes with 0.1% cyclosporin A. We investigated the changes of pupil diameter, intraocular pressure, and anterior chamber flare before and after applying the eye drops. We also examined the effect on ocular tissue histopathologically. RESULTS: Miosis was recognized in six eyes, but no significant intraocular pressure change was observed in any eyes. In both eyes of monkey A anterior chamber flare increased significantly, and flare increased slightly in both eyes of monkeys B, C, and D. On the other hand, there was no change in either eye of monkey E, including the control eye. Localized necrosis of nonpigmented ciliary epithelium was recognized at the beginning of the ciliary process in both eyes of monkey A. Mild cystoid degeneration of nonpigmented ciliary epithelium was seen at the beginning of the ciliary process in the right eye of monkey C, and in the left eye of monkey D. CONCLUSION: No species specificity can be recognized in monkeys from the fact that there is the selective destruction of nonpigmented epithelium at the beginning of the ciliary process after repeated instillation of 0.1% cyclosporin A eye drops, although there was a difference in miosis and anterior chamber inflammation-like reaction in individual monkeys.

Animals↗
PubMed2005

Macular hole secondary to epiretinal membrane in a juvenile patient.

PURPOSE: To report a macular hole secondary to an idiopathic epiretinal membrane in a 14-year-old girl. DESIGN: Interventional case report. METHODS: A 3-year-old girl who failed a public visual-screening test was examined. Since then, she had been followed-up for her epiretinal membrane, which began to peel spontaneously in the left eye for 11 years until a macular hole was observed. She was treated by vitrectomy and membranectomy, and the membrane was histologically examined. RESULT: The macular hole was successfully closed, and her visual acuity improved. The membrane appeared to have a homogenous structure and contained no cells. CONCLUSIONS: The juvenile idiopathic epiretinal membrane sometimes peels spontaneously. However, long-term follow-up is needed because vitreous traction of the membrane by intraocular aging change may result in a macular hole.

Child, Preschool↗
PubMed2005

[Case of eyelid nodular fasciitis in which magnetic resonance imaging was useful for clinical diagnosis].

BACKGROUND: Magnetic resonance imaging (MRI) is useful in pathogenesis, diagnosis, and treatment. CASE: A 70-year-old man. He noticed that the right side of his upper lid had swollen 3 weeks previously. No abnormal findings were recognized in visual acuity, intraocular pressure, anterior segment, lens, vitreous, or fundus. From MRI findings 10 days after his first visit, dermoid cyst, soft tissue tumor, and nodular fasciitis were suspected. Because MRI 2 months after the first visit showed a decrease in tumor size, soft tissue tumor was ruled out. Finally histopathological examination revealed nodular fasciitis, although the findings during the operation did not differentiate between dermoid cyst and nodular fasciitis. CONCLUSION: MRI at different intervals was useful for clinical diagnosis.

Aged↗
PubMed2005

[Cellular characteristics of cultured non-pigmented ciliary epithelium from adult pigs].

PURPOSE: To compare the reactive proliferation of non-pigmented ciliary epithelial cells in patients with cyclitis, or after cyclophotocoagulation and cyclocryocoagulation, and the cultured non-pigmented ciliary epithelial cells from adult pigs. METHODS: Porcine ciliary epithelial cells were cultured and non-pigmented ciliary epithelial cells were isolated. Detection of DNA synthesis, morphological observation by a phase contrast microscope and a transmission electron microscope, and staining of senescence-associated beta-galactosidase were carried out. RESULTS: The cells proliferated without showing contact inhibition of growth or reconstitution of epithelial morphology. With a decrease of proliferative activity, the cultured cells expressed senescence-associated beta-galactosidase. Although DNA synthesis persisted for a long time, some cells in later culture periods showed morphologically abnormal nuclei or plural nuclei indicating dysfunction of cell division, or apoptotic features. CONCLUSION: The uncontrolled growth and loss of the epithelial nature of non-pigmented ciliary epithelial cells in vitro resembles the process of formation of cyclitic membrane and proliferation of ciliary epithelium after cyclophotocoagulation and cyclocryocoagulation in patients. Observatin of the behavior of cultured non-pigment epithelial cells could aid in understanding the mechanism of cyclitic membrane formation.

Animals↗
PubMed2004

[The surgical outcome of vitrectomy for idiopathic epiretinal membranes and foveal thickness before and after surgery].

PURPOSE: To evaluate the surgical outcome of vitrectomy and foveal thickness before and after vitrectomy for idiopathic epiretinal membranes. SUBJECTS AND METHODS: Twenty-three eyes of 21 patients with idiopathic epiretinal membranes were analyzed. The mean follow-up period was 17.8 months. In 16 eyes of 14 patients, foveal thickness was measured by optical coherence tomography (OCT). RESULTS: Preoperative visual acuity ranged from 0.1 to 0.7. Postoperatively, 17 eyes achieved a final visual acuity of 1.0 or better, and 21 eyes achieved 0.5 or better. Preoperative foveal thickness ranged from 205 to 575 microns (mean +/- standard deviation, 409.9 +/- 103.3 microns). Postoperatively, mean foveal thickness was 347.7 +/- 106.0 microns (1 week follow up), 338.6 +/- 103.7 microns (2 months), 304.6 +/- 97.0 microns (6 months), and 274.3 +/- 78.7 microns (1 year). There was a weak negative correlation between visual acuity and foveal thickness. CONCLUSION: Visual acuity improves significantly after surgery. OCT is useful for idiopathic epiretinal membrane surgery.

Adult↗
PubMed2004

[Histopathologic study on cyclocryotherapy of human and monkey eyes].

PURPOSE: To evaluate the lesion and repair process of the ciliary body and adjacent tissues in glaucomatous human eyes and normal monkey eyes with cyclocryotherapy. METHODS: We used light and electron microscopy to observe the ciliary body and adjacent tissues in five glaucomatous human eyes and five normal monkey eyes which were given cyclocryotherapy. RESULTS: Five absolute glaucomatous eyes were given cyclocryotherapy from one and a half to 3 years before the enucleation. One eye had no coagulated spots on the ciliary process, but five eyes had coagulated spots on the pars plana. The pigment epithelial cells were atrophic or had disappeared, but non-pigment epithelial cells had proliferated by one or two layers. At the lesion of monkey pars plicata cyclocryotherapy up to three months, melanophage phagocytosed pigment epithelium and melanocytes accumulated, although the proliferation of non-pigment epithelial cells was seen. At the lesion of monkey pars plana cyclocryotherapy melanophage accumulation was also marked, although non-pigment epithelial proliferation was greater than in pars plicata cyclocryotherapy. CONCLUSION: As cyclocryotherapy is a blind therapy, it is uncertain and difficult to destroy the ciliary process precisely. The repair process continued up to three months after monkey cyclocryotherapy, but it took up to one year and 6 months after human cyclocryotherapy.

Adult↗
PubMed2004

[Clinicopathological study on suprachoroidal and supraciliary hemorrhage during enucleation].

PURPOSE: To investigate the pathogenesis of suprachoroidal and supraciliary hemorrhage that might have been induced during enucleation. METHODS: A histopathological examination of 392 enucleated eyeballs was carried out and 8 eyeballs with suprachoroidal and supraciliary hemorrhage were selected for further clinicopathological examination. RESULTS: Among 14 eyeballs with severe acute intraocular inflammation, 7 eyeballs with suprachoroidal and supraciliary hemorrhage were found and one other such eyeball was seen among 53 with neovascular glaucoma. Among these 8, there was one case of prolapse of intraocular tissue with severe hemorrhage into the suprachoroidal and supraciliary spaces; 4 cases of severe hemorrhage into the suprachoroidal and supraciliary spaces without prolapse of intraocular tissue; and 3 cases of mild hemorrhage into the suprachoroidal or supraciliary spaces. CONCLUSIONS: In the eyeballs with severe acute intraocular inflammation, intraocular pressure was elevated and the blood vessels were weakened by inflammatory cell infiltration. During enucleation external forces affected the blood vessel wall of the ciliary arteries and vortex veins, and the breakdown of vessel walls might have been the cause of the suprachoroidal and supraciliary hemorrhage.

Acute Disease↗