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Shih-Wen Huang

Publications and source records attributed to Shih-Wen Huang.

11 recordsLinked to original sources
PubMed2006

Molecular and immunological characterization of allergens from the entomopathogenic fungus Beauveria bassiana.

BACKGROUND: Entomopathogenic fungi such as Beauveria bassiana are considered promising biological control agents for a variety of arthropod pests. Beauveria species, however, have the potential to elicit allergenic reactions in humans, although no specific allergens have been characterized to date. METHODS: Four putative allergens were identified within B. bassiana expressed sequence tag (EST) datasets. IgE-reactivity studies were performed using sera from patients displaying mold allergies against recombinant B. bassiana proteins expressed in E. coli. RESULTS: Full length cDNA and genomic nucleotide sequences of four potential B. bassiana allergens were isolated. BLASTX search results led to their putative designation as follows; Bb-Eno1, with similarity to fungal enolases; Bb-f2, similar to the Aspergillus fumigatus major allergen, Asp f2 and to a fibrinogen binding mannoprotein; Bb-Ald, similar to aldehyde dehydrogenases; and Bb-Hex, similar to N-acetyl-hexosaminadases. All four genes were cloned into E. coli expression systems and recombinant proteins were produced. Immunoblots of E. coli extracts probed with pooled as well as individual human sera from patients displaying mould allergies demonstrated IgE reactivity versus recombinant Bb-Eno1 and Bb-Ald. CONCLUSION: Four putative Beauveria bassiana allergens were identified. Recombinant proteins corresponding to two of the four, Bb-Eno1 and Bb-Ald were bound by sera IgEs derived from patients with fungal allergies. These data confirm the potential allergenicity of B. bassiana by identification of specific human IgE reactive epitopes.

Journal Article↗
PubMed2005

Allergens of the entomopathogenic fungus Beauveria bassiana.

BACKGROUND: Beauveria bassiana is an important entomopathogenic fungus currently under development as a bio-control agent for a variety of insect pests. Although reported to be non-toxic to vertebrates, the potential allergenicity of Beauveria species has not been widely studied. METHODS: IgE-reactivity studies were performed using sera from patients displaying mould hypersensitivity by immunoblot and immunoblot inhibition. Skin reactivity to B. bassiana extracts was measured using intradermal skin testing. RESULTS: Immunoblots of fungal extracts with pooled as well as individual sera showed a distribution of IgE reactive proteins present in B. bassiana crude extracts. Proteinase K digestion of extracts resulted in loss of IgE reactive epitopes, whereas EndoH and PNGaseF (glycosidase) treatments resulted in minor changes in IgE reactive banding patterns as determined by Western blots. Immunoblot inhibitions experiments showed complete loss of IgE-binding using self protein, and partial inhibition using extracts from common allergenic fungi including; Alternaria alternata, Aspergillus fumigatus, Cladosporium herbarum, Candida albicans, Epicoccum purpurascens, and Penicillium notatum. Several proteins including a strongly reactive band with an approximate molecular mass of 35 kDa was uninhibited by any of the tested extracts, and may represent B. bassiana specific allergens. Intradermal skin testing confirmed the in vitro results, demonstrating allergenic reactions in a number of individuals, including those who have had occupational exposure to B. bassiana. CONCLUSIONS: Beauveria bassiana possesses numerous IgE reactive proteins, some of which are cross-reactive among allergens from other fungi. A strongly reactive potential B. bassiana specific allergen (35 kDa) was identified. Intradermal skin testing confirmed the allergenic potential of B. bassiana.

Journal Article↗
PubMed2005

Role of renal sonography in the intensive care unit.

PURPOSE: This study was conducted to evaluate the role of portable renal sonography in the intensive care unit (ICU). METHODS: We conducted a retrospective study of 402 ICU patients who underwent renal sonography. We recorded demographic data, underlying disease, type of ICU, renal function test results, etiology of renal failure, need for dialysis, and outcome for patients with acute renal failure (ARF). The indications for and results of sonography were analyzed. RESULTS: The most common indication for a renal sonographic examination was ARF (320/402, 79.6%). Hydronephrosis was found in 5 patients with ARF. Chronic renal failure was confirmed by sonography in 40% of the patients with an indeterminate cause of renal failure. In 33% of cases of complicated urinary tract infections, sonography revealed abnormalities. Renal sonography was also useful for follow-up assessment of patients treated with percutaneous nephrostomy and patients with a history of renal tumor, hydronephrosis, adrenal tumor, hematuria of unknown cause, or fever of unknown origin. CONCLUSIONS: Since renal disease is common in the ICU, renal sonography is a convenient and useful diagnostic tool in this setting.

Acute Kidney Injury↗
PubMed2005

Sub-proteome analysis of novel IgE-binding proteins from Bermuda grass pollen.

Bermuda grass (Cynodon dactylon) pollen (BGP) is one of the most common causes of airway allergic disease, and has been shown to contain over 12 allergenic proteins on 1-D immunoglobulin E (IgE) immunoblots. However, only a few allergens have been identified and characterized. Cyn d 1 is a major allergen and the most abundant protein in BGP, representing 15% of the whole-pollen extract. To investigate variability in the IgE-reactive patterns of BGP-sensitized patients and to identify other prevalent allergens, a BGP extract was passed through an affinity column to remove Cyn d 1, and the non-bound material was collected and analyzed by 2-DE. IgE-reactive proteins were subsequently characterized by immunoblotting using serum samples from ten BGP-allergic patients. The prevalent IgE-reactive proteins were identified by MALDI-TOF MS, N-terminal sequence similarity, and LC-MS/MS. Here, we present a sub-proteome approach for allergen investigation and its use for determining BGP 2-DE profiles and identifying six novel allergens.

Blotting, Western↗
PubMed2005

Purification and structural analysis of the novel glycoprotein allergen Cyn d 24, a pathogenesis-related protein PR-1, from Bermuda grass pollen.

Bermuda grass pollen (BGP) contains a very complex mixture of allergens, but only a few have been characterized. One of the allergens, with an apparent molecular mass of 21 kDa, has been shown to bind serum IgE from 29% of patients with BGP allergy. A combination of chromatographic techniques (ion exchange and reverse phase HPLC) was used to purify the 21 kDa allergen. Immunoblotting was performed to investigate its IgE binding and lectin-binding activities, and the Lysyl-C endopeptidase digested peptides were determined by N-terminal sequencing. The cDNA sequence was analyzed by RACE PCR-based cloning. The protein mass and the putative glycan structure were further elucidated using MALDI-TOF mass spectrometry. The purified 21 kDa allergen was designated Cyn d 24 according to the protocol of International Union of Immunological Societies (IUIS). It has a molecular mass of 18,411 Da by MALDI-TOF analysis and a pI of 5.9. The cDNA encoding Cyn d 24 was predicted to produce a 153 amino acid mature protein containing tow conserved sequences seen in the pathogen-related protein family. Carbohydrate analysis showed that the most abundant N-linked glycan is a alpha(3)-fucosylated pauci-mannose (Man3GlcNAc2) structure, without a Xyl beta-(1,2)-linked to the branching beta-Man. Thus, Cyn d 24 is a glycoprotein and the results of the sequence alignment indicate that this novel allergen is a pathogenesis-related protein 1. To the best of our knowledge, this is the first study to identify any grass pollen allergen as a pathogenesis-related protein 1.

Allergens↗
PubMedDate not supplied

Purification and characterization of a novel isoallergen of a major Bermuda grass pollen allergen, Cyn d 1.

A novel immunoreactive isoallergen of a major Bermuda grass pollen allergen, Cyn d 1, was purified by the use of a combination of various chromatographic techniques, including high-performance liquid chromatography. This new isoallergen has a pI value of 9.1 and shows significant N-terminal sequence homology with other isoforms. Carbohydrate composition analysis revealed a 10.4% carbohydrate content consisting of 7 different sugar moieties, including arabinose, fucose, galactose, glucose, mannose, xylose and N-acetylglucosamine, as well as a trace amount of rhamnose. Upon periodate oxidation, the binding activities of the Cyn d 1 isoform to murine monoclonal antibodies and human serum IgE and IgG were reduced, suggesting the importance of the carbohydrate moiety in the immune response. The availability of the purified Cyn d 1 basic isoform will allow for further structural and immunological characterization, and ultimately for the design of an appropriate therapy.

Allergens↗
PubMedDate not supplied

A call for establishing a Children's Environmental Health Information Resource in Taiwan.

A tragic case of 7 years old child who suffered from injury caused by chemical burn of esophagus uncovered an urgent need of public education on the labeling of reused medicine bottle. The sad fact is that the case presented in the article or similer cases which occurred in the past in children is largely preventable. This article makes a plead to pediatricians that as the guardians of children, we have an inevitable responsibility of educating the public about the prevention of chemical injury. To achieve this goal, by using the information recently available from the Center of Disease Control (CDC) of the United States. I suggest several possible steps that we can take to establish a Children's Environmental Health Information Resource in Taiwan.

Accident Prevention↗
PubMedDate not supplied

Results of an on-line survey of patients with hereditary angioedema.

Hereditary angioedema (HAE) is a rare but potentially fatal disease. An on-line survey was conducted of 63 patients in order to analyze the current clinical status of these patients. Sixty percent (n = 38) had type 1 HAE, 16% (n = 10) had type 2 HAE, and 24% (n = 15) did not know their type. The mean and median ages of onset of symptoms were predominantly in the teenage years. The diagnosis was typically made from 3.8 to 7.8 years after the onset of symptoms. The most common symptoms were abdominal pain and swelling (70%), swelling of extremities (45%), and throat closure (25%). Allergy-like symptoms were present in only 11.7% (n = 7) of patients; hypotension occurred in only one patient (1.6%). The patients averaged 4.7 emergency room visits per year. Nearly one-quarter of the patients were treated for anaphylaxis in the emergency room. There was a high level of dissatisfaction among the patients with the current management of the disease.

Adult↗
PubMedDate not supplied

Seafood and iodine: an analysis of a medical myth.

There is a prevailing myth that iodine levels in seafood and seafood allergy are connected. Therefore, we designed a study to collect information about this misconception from patients referred to our pediatric allergy clinic because of suspected seafood allergy. We presented five questions to our patients, and the most surprising result was that the majority of them believe that iodine is linked to seafood allergy. As a result, many felt uneasy about the use of iodine radiocontrast media. A survey of iodine content in common foods showed that, although the iodine content of seafood is higher than nonseafood items, daily consumption of the latter is much greater and, therefore, any phobia about iodine in seafood is unfounded. We encourage strong public education about seafood allergy by allergy specialists.

Contrast Media↗