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Shokichi Ohuchi

Publications and source records attributed to Shokichi Ohuchi.

2 recordsLinked to original sources
PubMed2006

Estimation of DNA polymerase for improvement of rolling circle amplification.

Rolling Circle Amplification (RCA) is a technique to amplify the oligoncleotides containing tandemly repetitive sequence motif. Various repetitive sequences of which the motif unit is the circular template can be synthesized by extending the primer. Additionally, RCA need not change the temperature regulatory processes like PCR, but RCA has the gene amplification efficiency similar to PCR. Although, it contains the problem of the sequence confirmation and the activity of polymerase as the DNA polymerase I. We comparatively estimated of the various polymerases for the improvement of the RCA. Five DNA polymerases were estimated for RCA.

DNA↗
PubMed2006

Microwave assisted rolling circle amplification.

The Rolling Circle Amplification is an effective method of DNA amplification having tandem repeated sequences. Firstly, the template sequence is designed to form a circular structure by the annealing with the primer. The thermostable polymerase is catalyzed to primer and circular template DNA. As a result, tandem repetitive olignucleotide of circular template sequence can be obtained. In our recent study, Bst DNA polymerase catalyzed RCA reaction had been elucidated that the oligonucleotides with tandem repeated sequence were obtained as the ladder band and amplified the enough amounts of DNA within short time. On the other hand, we also focused on the microwave technology for advance to the various chemical and biological reactions. In this study, the microwave was irradiated to RCA reaction on controlling the temperature.

DNA↗