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Shu-Hong Zhang

Publications and source records attributed to Shu-Hong Zhang.

5 recordsLinked to original sources
PubMed2006

Cloning, expression and functional study of translation elongation factor 2 (EF-2) in zebrafish.

We have identified translation elongation factor 2 (EF-2) in zebrafish (GenBank Accession No. AAQ91234). Analysis of the DNA sequence of zebrafish EF-2 shows that the 2826 bp cDNA spans an open reading frame between nucleotide 55 to 2631 and encodes a protein of 858 amino acids. Zebrafish EF-2 protein shares 92%, 93%, 93% and 92% identity with the corresponding amino acid sequence in human, mouse, Chinese hamster and Gallus EF-2, respectively. Whole-mount in situ hybridization showed that zebrafish EF-2 was a developmentally regulated gene and might play important roles during the early development of zebrafish embryos. Therefore, we further studied the function of EF-2 during early embryogenesis. Using morpholino antisense oligo knockdown assays, anti-MO injected embryos were found to display abnormal development. The yolk balls were larger than normal and the melanophores spreading on their bodies became fewer. Furthermore, their tails were incurvate and their lenses were much smaller than those of the normal embryos. However the EF-2 overexpression data showed that extra EF-2 protein had no obvious effect on zebrafish embryonic development.

Amino Acid Sequence↗
PubMed2005

Growth of silver nanowires from solutions: a cyclic penta-twinned-crystal growth mechanism.

Silver nanowires are synthesized by simple reduction of the silver ions with reductants such as glucose, sodium citrate, and sodium hypophosphite, etc., in the absence of the so-called surfactants or capping reagents at the temperature from 80 to 200 degrees C. Regardless of the reductants, the nanowires prepared at a given temperature are uniform in diameters, ranging from 30 to 50 nm at 100 degrees C. Nanoparticles coexist with nanowires in the products with larger diameters (usually larger than 50 nm). We find that all the silver nanowires in the as-prepared products are of cyclic penta-twinned structure, where five crystallites bond by the {111} facets. We propose that the intrinsic factor of the cyclic penta-twinned structure, i.e., the angular mismatch of the five crystallites in forming a gapless rod, controls the size of the nanowires and guides the directional growth of the nanowires with {110} as the active facets. The nanoparticles in the products are aggregates of imperfect penta-twinned crystals, which inhibits them from growing into nanowires and results in larger size. From the structural information of the nanoparticles synthesized at room temperature, we propose that the formation of the cyclic penta-twinned structure is due to the stacking fault and the intrinsic equilibrium structures of the lower energy.

Journal Article↗