Biomedical subjects

Shujin Zhao

Publications and source records attributed to Shujin Zhao.

8 recordsLinked to original sources

PubMed2005

[Expression and assay in vitro of phage single chain antibody against nucleoprotein of influenza virus A type].

OBJECTIVE: To expression the phage single chain antibody against nucleoprotein of influenza virus A type in E. coli strain HB2151 by screening the positive clone from the phage antibody library against nucleoprotein, which will prepare for the construction of fast leak kit of Influenza virus A type. METHODS: The positive clone ratio in the phage antibody library was enriched by three-round continual solid panning, ELISA, SDS-PAGE and Western-blot methods were used to analyze the protein secreted into the supemants and periplasmic. Affinity chromatography was used to purified the protein expressed in periplasmic and the titer was also analyzed using ELISA method. RESULTS: 10 clones were screened from96 clones and among the 10, there were 3 stronger positive with OD450nm value of 0.469, 0.582 and 0.507, respectively. The phage single chain antibody against Influenza virus A type was primary expressed in periplasmic. The ELISA results were positive even if the single chain antibody purified by affinity chromatography was diluted 4096 folds. CONCLUSION: Using phage antibody library technique, phage single antibody against nucleoprotein of influenza virus A type was preliminary expressed in E. coli.

Antibodies, Viral↗

PubMed2005

[Evaluation on construction and expression in vitro of nucleic acid vaccine of nucleoprotein of influenza virus A].

OBJECTIVE: Constructing nucleic acid vaccine of influenza virus A to study the protective effects. METHODS: NP gene was reverse transcripted from influenza virus A and linked with pSECTAG 2/Hygro A to constructed nucleic acid vaccine of influenza virus A. The constructed nucleic acid vaccine was transinfected into VERO cell resorting to lipofectamineTM2000. NP gene of influenza virus A was expressed in VERO cell by the method of ELISA. RESULTS: The results showed that the NP gene was expressed to the highest level at 36h after transinfection with the A40 value of 0.382. From 36 h to 60 h after transinfection, the expression of NP gene was stable (the A45, value at 48h and 60h was 0.385 and 0.387, respectively) . CONCLUSION: The nucleic acid vaccine of influenza virus A was successfully constructed, which sets up groudworks for the research of effection of nucleic acid vaccine in vivo.

Animals↗

PubMed2004

[Expression and identification of cecropin D gene cloned in Pichia pastoris yeast cells].

OBJECTIVE: Cecropin D gene was cloned into methanol nutritional vector-pPICZ alpha-A, linked with alpha-factor signal peptide. METHODS: Positive recombinant was gotten through PCR and EcoR I restriction enzyme. The positive recombinant vector was transformed into Pichia pastoris yeast strain GS115. RESULTS: The positive recombinant yeast velum was fermented and its expressive product was assayed by the method of anti-germ. CONCLUSION: Cecropin D gene was cloned into yeast cell and its expressive products has high activity of killing bacteria, Which could be developed into a new type of anti-bacterial medicine.

Anti-Bacterial Agents↗

PubMed2004

[Mechanism and prevention measures of gene silencing].

With the widely application of transgene technology in the fields of high-level biologies, more and more attention was payed to gene silence. Gene silence can take place at the phase of transcription and post-transcription. With the results of methylation, repeated copies and homologous sequence of inner genes, trans-gene are silenced. There are many kinds of mechanisms of gene silencing and here, the mechanisms explaining the gene silence and some prevention measures were summarized in this paper.

DNA Methylation↗

PubMed2004

[Progress on phage antibody library technology].

The exterior protein gene and the specific protein gene coded on the surface of phage can be fusedly expressed on the surface of phage with the recently developed technology-phage display technique. Through the PCR technique, the whole light chain genes and heavy chain genes can be amplified. Cloning these genes into lambda phage expression vectors and constructing phage antibody library through antigen adsorption-elution-proliferation, many targeting clones could be selected and the responding mono-clone antibody could be produced. Detailed progress in this regard was reviewed in the paper.

Antibodies, Monoclonal↗

PubMed2004

[Purification of chymopapain and its effect on nucleus pulposus in vitro].

OBJECTIVE: To establish a simple and effective procedure for purification of chymopapain and study about its effect on nucleus pulposus in vitro. METHODS: Chymopapain was purified by ion exchange chromatograph and tested its effect on nucleus pulposus in vitro. RESULTS: A simple and effective procedure for purification of chymopapain was established and the chymopapain did degrade nucleus pulposus. CONCLUSION: The ion exchange chromatograph was a simple and effective procedure for purification of chymopapain. It is necessary to study its effect on nucleus pulposus in vivo.

Asimina↗

PubMed2003

[Gene chip technology and it's application to genetic toxicology].

Gene chip, also called as DNA array, Oligonucleotide Microchip or Oligonucletide array, is a new technology developed in recent years. It can be applied prospectively in life science research, such as gene expression, gene diagnose and gene polymorphism etc. In this paper, the principle, types, procedures and application of gene chip to genetic toxicological research were reviewed.

Humans↗

PubMed2002

[The preparation and identification of rabbit's antibody of chymopapain].

Chymopapain, one of the four cysteine proteinases of papaya latex, has milk clotting and proteolytic activity. It is mainly used to treat prolapsed intervertebral discs. This paper introduced the preparation of polyclonal antibody of partially purified chymopapain and the identification of the antibody specificity. The polyclonal antibody provides a basis for the further researches and applications of chymopapain.

Animals↗