Biomedical subjects

Simon E Moulton

Publications and source records attributed to Simon E Moulton.

5 recordsLinked to original sources

PubMed2006

Optimising the incorporation and release of a neurotrophic factor using conducting polypyrrole.

In this study, a neurotrophin delivery system based on an inherently conducting polymer (ICP) has been developed. Direct incorporation of neurotrophin-3 (NT-3) was investigated and controlled release was tested under various electrochemical conditions. The loading capacity and amount of NT-3 released from the polymer was determined using (125)I-labelled NT-3. Electrochemical stimulation of polypyrrole by pulsed voltage, pulsed current or cyclic voltammetry promoted the release of NT-3 at a greater rate than natural diffusion of NT-3. NT-3 was released from polypyrrole as an initial burst in the first 24 h followed by prolonged release over a subsequent 6 days of sampling. The amount of NT-3 incorporated into the polymer could be controlled by varying the polymerisation time, with longer growth periods incorporating more NT-3. The NT-3 release results indicated that the polymers grown for longer released a lower percentage of the incorporated NT-3 compared to the polymers grown for shorter times. Polymer-based neurotrophin delivery systems have the potential to be incorporated into future treatments for nerve injuries to prevent nerve degradation and promote nerve protection.

Delayed-Action Preparations↗

PubMed2005

Optically active polymer carbon nanotube composite.

A completely soluble optically active polyaniline-multiwalled carbon nanotube composite was investigated by spectroscopic and microscopic techniques. It was found that the polymer's optical activity was retained in the presence of carbon nanotubes. Solutions were found to be easily processable into thin films, which exhibited dendritic structures only in the presence of nanotubes.

Journal Article↗

PubMed2005

Investigation of Ig.G adsorption and the effect on electrochemical responses at titanium dioxide electrode.

The adsorption of Immunoglobulin G on a titanium dioxide (TiO(2)) electrode surface was investigated using (125)I radiolabeling and electrochemical impedance spectroscopy (EIS). (125)I radiolabeling was used to determine the extent of protein adsorption, while EIS was used to ascertain the effect of the adsorbed protein layer on the electrode double layer capacitance and electron transfer between the TiO(2) electrode and the electrolyte. The adsorbed amounts of Ig.G agreed well with previous results and showed approximately monolayer coverage. The amount of adsorbed protein increased when a positive potential was applied to the electrode, while the application of a negative potential resulted in a decrease. Exposure to solutions of Ig.G resulted in a decrease of the double layer capacitance (C) and an increase in the charge-transfer resistance (R(2)) at the electrode solution interface. As more Ig.G adsorbed onto the electrode surface, the extent of C and R(2) variation increased. These capacitance and charge-transfer resistance variations were attributed to the formation of a proteinaceous layer on the electrode surface during exposure.

Adsorption↗

PubMed2004

Use of inherently conducting polymers and pulsed amperometry in flow injection analysis to detect oligonucleotides.

Pulsed amperometry has been used to detect and discriminate between different base-pair oligonucleotides using flow injection analysis with polypyrrole modified electrodes. Oligonucleotides with 20-repeat units were immobilized as the sole counter anion during polymerisation of pyrrole onto a glassy carbon (GC) electrode. The modified electrode was then used in a flow injection analysis (FIA) set-up to selectively detect complementary oligonucleotides. Results showed that complementary oligo(dG)(20) and non-complementary oligonucleotides can be distinguished by using the oligo(dC)(20) modified electrode.

Animals↗

PubMed2004

Stabilization of single-wall carbon nanotubes in fully sulfonated polyaniline.

The interaction of single wall carbon nanotubes (SWNT) with an aqueous solution of the fully sulfonated polyaniline poly(2-methoxyaniline-5-sulfonic acid) (PMAS) and (+)-1-phenylethylamine (PhEA) has been investigated using spectroscopic methods. UV-vis spectral measurements show that the PMAS backbone undergoes conformational changes upon interaction with both SWNT and PhEA. Partial intercalation of PMAS into SWNTbundles was confirmed by Raman spectroscopy and electron microscopy.

Adsorption↗