Biomedical subjects

Siqi Huang

Publications and source records attributed to Siqi Huang.

2 recordsLinked to original sources

PubMed2025

Molecular mechanism of HaiZao-YuHu decoction in breast cancer treatment via network pharmacology and molecular docking: Computational pharmacology.

BACKGROUND: The molecular biological mechanisms of HaiZao-YuHu decoction were investigated using network pharmacology and molecular docking. METHODS: TCMSP database was used to collect the active ingredients and action targets of HaiZao-YuHu decoction, through the OMIM, PharmGkb, GeneCards, TDD, and DurgBank database query targets for breast cancer. Then, using the intersecting targets, the protein-protein interaction network of HaiZao-YuHu decoction was constructed using the STRING website. Network topology analysis was performed using Cytoscape 3.9.0 to identify the core targets. Gene ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses were performed with the R package. The Autodock software was used for molecular docking. RESULTS: Thirty-four active ingredients, 219 intersection targets and 4 key targets were obtained. gene ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis get 2152 biological processes and 186 pathways. Molecular docking showed that the 4 core targets could combine well with the 5 main active components. CONCLUSION: HaiZao-YuHu decoction can play a role in the treatment of breast cancer through multi-targets, multi-components, and multi-pathways.

Molecular Docking Simulation↗

PubMed2024

Gradient-Elution Nanoflow Liquid Chromatography Without a Binary Pump: Smoothed Step Gradients Enable Reproducible, Sensitive, and Low-Cost Separations for Single-Cell Proteomics.

Mass spectrometry-based proteome profiling of trace analytes including single cells benefits from liquid chromatography separations operated at low flow rates (e.g., <50 nl/min). However, high-pressure binary pumps needed to achieve such flow rates are not commercially available, and instead require splitting of the gradient flow to achieve low-nanoliter-per-minute flow rates. Gradient flow splitting can waste solvent and lead to flow inconsistencies. To address this, we have developed a method for creating gradients by combining segments of mobile phase having increasing solvent strength together in an open capillary, and then relying on Taylor dispersion to form the desired smooth gradient profile. Our method dramatically reduces costs, as only a single isocratic high-pressure pump is required. Following development of gradient profiles for both 10- and 20-min active gradients, we measured 200 pg injections of HeLa digest using a timsTOF mass spectrometer. Finally, we investigated differences in protein expression between single cells originating from two different colonies of ATG-KO HeLa cells. Thousands of proteins were quantified, and a potential mechanism explaining differential immune responses of these two colonies upon exposure to viral DNA treatment was determined.

Humans↗