Biomedical subjects

Sophie Bourgeon

Publications and source records attributed to Sophie Bourgeon.

4 recordsLinked to original sources

PubMed2006

Innate immunity, assessed by plasma NO measurements, is not suppressed during the incubation fast in eiders.

Immunity is hypothesized to share limited resources with other physiological functions and may mediate life history trade-offs, for example between reproduction and survival. However, vertebrate immune defense is a complex system that consists of three components. To date, no study has assessed all of these components for the same animal model and within a given situation. Previous studies have determined that the acquired immunity of common eiders (Somateria mollissima) is suppressed during incubation. The present paper aims to assess the innate immune response in fasting eiders in relation to their initial body condition. Innate immunity was assessed by measuring plasma nitric oxide (NO) levels, prior to and after injection of lipopolysaccharides (LPS), a method which is easily applicable to many wild animals. Body condition index and corticosterone levels were subsequently determined as indicators of body condition and stress level prior to LPS injection. The innate immune response in eiders did not vary significantly throughout the incubation period. The innate immune response of eiders did not vary significantly in relation to their initial body condition but decreased significantly when corticosterone levels increased. However, NO levels after LPS injection were significantly and positively related to initial body condition, while there was a significant negative relationship with plasma corticosterone levels. Our study suggests that female eiders preserve an effective innate immune response during incubation and this response might be partially determined by the initial body condition.

Animals↗

PubMed2006

Phytohemagglutinin response and immunoglobulin index decrease during incubation fasting in female common eiders.

To maximize their fitness, long-lived species face trade-offs between survival and reproduction. The cost of reproduction, which is defined as the negative effect of current parental investment on chances of adult survival and future reproduction, may affect immune function, possibly through hormonal changes. In this study, components of acquired immunity and plasma corticosterone levels of female eiders (Somateria mollissima) have been measured throughout the incubation period as a function of clutch size. These precocial birds lay up to six eggs and fast completely during incubation. Birds were sampled early and late in the incubation period, with clutches ranging from one to four eggs. T-cell-mediated immune response and humoral immunity were assessed by phytohemagglutinin (PHA) skin tests (a challenging method) and measurements of serum immunoglobulins (a monitoring method), respectively. During incubation, responses to PHA injection and immunoglobulin index significantly decreased, by about 40% and 25%, respectively. These observed decreases occurred independently of the number of eggs laid by the females. Corticosterone did not vary significantly during incubation, whatever the clutch size. We conclude that female eiders seem to reallocate their resources from immune function to reproductive effort independently of clutch size or corticosterone levels.

Animals↗

PubMed2006

Fasting-induced changes of immunological and stress indicators in breeding female eiders.

One adaptive significance of immunosuppression during reproduction can be explained by the immunopathology-avoidance hypothesis. This hypothesis states that since heat shock proteins (HSP) are highly conserved proteins found in both pathogen and host, and are expressed at a higher level during reproduction, the risk of autoimmunity is then increased, HSP being the target of the host's immune response. Reduced immunocompetence has been attributed to hormonal regulation, in particular by glucocorticoids. The current study aimed at testing the immunopathology-avoidance hypothesis and the implication of corticosterone in incubating fasting common eiders (Somateria mollissima). To this end, we have measured immunological and stress indicators including immunoglobulin (IgY), HSP70, HSP60, and corticosterone levels in breeding females. A multivariate general linear model analysis showed that female body condition, IgY, HSP70, and HSP60 levels were the main variables explaining the model. Females showed a significant decrease by 15% of their IgY index during incubation. Conversely, HSP70 and HSP60 levels significantly increased by 12 and 10%, respectively throughout incubation. Moreover, there was a positive significant relationship between both HSP whereas HSP60 levels were negatively correlated to IgY index. Plasma corticosterone levels showed a tendency to decrease during incubation. We conclude that these findings are consistent with the immunopathology-avoidance hypothesis in breeding eiders. Nevertheless, the long-term reproductive costs and the underlying mechanisms of such an immunosuppression remain to be determined and will require further experiments.

Animals↗

PubMed2006

Corticosterone selectively decreases humoral immunity in female eiders during incubation.

Immunity is hypothesized to share limited resources with other physiological functions and this may partly account for the fitness costs of reproduction. Previous studies have shown that the acquired immunity of female common eider ducks (Somateria mollissima) is suppressed during their incubation, during which they entirely fast. Corticosterone was proposed to be an underlying physiological mechanism for such immunosuppression. Therefore, the current study aimed to assess the effects of exogenous corticosterone on acquired immunity in captive eiders. To this end, females were implanted with corticosterone pellets at different stages of their incubation fast. We measured total immunoglobulin levels, T-cell-mediated immune response, body mass and corticosterone levels in these females and compared them with those of control females prior to and after manipulation (i.e. corticosterone pellet implantation). To mimic corticosterone effects on body mass, we experimentally extended fasting duration in a group of females termed ;late fasters'. Implanted females had corticosterone levels 6 times higher and lost 35% more mass than control females. Corticosterone levels in ;late fasters' were similar to those in control females but body mass was 8% lower in the former. The decrease in the immunoglobulin levels of corticosterone implanted females was twice as high as in control females, while the T-cell-mediated immune response was not significantly affected by the treatment. We found a decrease in the T-cell-mediated immune response only in ;late fasters' (by 60%), while the immunoglobulin level was not lower in this group than in corticosterone implanted or control females. Our study shows that in incubating eiders, exogenous corticosterone only decreased humoral immunity. We suggest that the immunosuppressive effect of corticosterone could be mediated through its effects on body reserves. Further experiments are required to determine the relationship between body condition and immune system in fasting birds.

Animals↗