Biomedical subjects

Stefan Klumpp

Publications and source records attributed to Stefan Klumpp.

5 recordsLinked to original sources

PubMed2005

Active diffusion of motor particles.

The movement of motor particles consisting of one or several molecular motors bound to a cargo particle is studied theoretically. The particles move on patterns of immobilized filaments. Several patterns are described for which the motor particles undergo nondirected but enhanced diffusion. Depending on the walking distance of the particles and the mesh size of the patterns, the active diffusion coefficient exhibits three different regimes. For micrometer-sized motor particles in water, e.g., this diffusion coefficient can be enhanced by 2 orders of magnitude.

Computer Simulation↗

PubMed2005

Cooperative cargo transport by several molecular motors.

The transport of cargo particles that are pulled by several molecular motors in a cooperative manner is studied theoretically in this article. The transport properties depend primarily on the maximal number N of motor molecules that may pull simultaneously on the cargo particle. Because each motor must unbind from the filament after a finite number of steps but can also rebind to it again, the actual number of pulling motors is not constant but varies with time between zero and N. An increase in the maximal number N leads to a strong increase of the average walking distance (or run length) of the cargo particle. If the cargo is pulled by up to N kinesin motors, for example, the walking distance is estimated to be 5(N-1)/N micrometers, which implies that seven or eight kinesin molecules are sufficient to attain an average walking distance in the centimeter range. If the cargo particle is pulled against an external load force, this force is shared between the motors, which provides a nontrivial motor-motor coupling and a generic mechanism for nonlinear force-velocity relationships. With increasing load force, the probability distribution of the instantaneous velocity is shifted toward smaller values, becomes broader, and develops several peaks. Our theory is consistent with available experimental data and makes quantitative predictions that are accessible to systematic in vitro experiments.

Biological Transport↗

PubMed2005

Self-organized density patterns of molecular motors in arrays of cytoskeletal filaments.

The stationary states of systems with many molecular motors are studied theoretically for uniaxial and centered (asterlike) arrangements of cytoskeletal filaments using Monte Carlo simulations and a two-state model. Mutual exclusion of motors from binding sites of the filaments is taken into account. For small overall motor concentration, the density profiles are exponential and algebraic in uniaxial and centered filament systems, respectively. For uniaxial systems, exclusion leads to the coexistence of regions of high and low densities of bound motors corresponding to motor traffic jams, which grow upon increasing the overall motor concentration. These jams are insensitive to the motor behavior at the end of the filament. In centered systems, traffic jams remain small and an increase in the motor concentration leads to a flattening of the profile if the motors move inwards, and to the buildup of a concentration maximum in the center of the aster if motors move outwards. In addition to motor density patterns, we also determine the corresponding patterns of the motor current.

Adenosine Triphosphate↗

PubMed2004

Asymmetric simple exclusion processes with diffusive bottlenecks.

One-dimensional asymmetric simple exclusion processes (ASEPs) that are coupled to external reservoirs via diffusive transport are studied. These ASEPs consist of active compartments characterized by directed movements of the particles and diffusive compartments in which the particles undergo unbiased diffusion. Phase diagrams are obtained by a self-consistent mean field approach and by Monte Carlo simulations. The diffusive compartments act as diffusive bottlenecks if the velocity of the driven compartments or ASEPs is sufficiently large. A diffusive bottleneck at the boundary of the system leads to the absence of a maximal current phase, while a diffusive bottleneck in the interior of the system leads to a new phase characterized by different densities in the two active compartments adjacent to the diffusive one and to a maximal current defined by the bottleneck.

Journal Article↗

PubMed2004

Random walks of molecular motors arising from diffusional encounters with immobilized filaments.

Movements of molecular motors on cytoskeletal filaments are described by directed walks on a line. Detachment from this line is allowed to occur with a small probability. Motion in the surrounding fluid is described by symmetric random walks. Effects of detachment and reattachment are calculated by an analytical solution of the master equation in two and three dimensions. Results are obtained for the fraction of bound motors, their average velocity, displacement, and dispersion. The analytical results are in good agreement with results from Monte Carlo simulations and confirm the behavior predicted by scaling arguments. The diffusion coefficient parallel to the filament becomes anomalously large since detachment and subsequent reattachment, in the presence of directed motion of the bound motors, leads to a broadening of the density distribution. The occurrence of protofilaments on a microtubule is modeled by internal states of the binding sites. After a transient time, all protofilaments become equally populated.

Adsorption↗