Simple dosage guide for suncreans will help users.
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Guidelines as Topic↗
Biomedical subjects
Steve Taylor
Publications and source records attributed to Steve Taylor.
Simple, effective ear syringing.
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Cerumen↗
Reversed-phase HPLC of polymer additives with multiple on-line spectroscopic analysis (UV, IR, (1)H NMR and MS).
The reversed-phase chromatography of a number of polymer additives has been undertaken with on-line characterisation via a combination of diode array UV, (1)H NMR, IR spectroscopy and mass spectrometry. This combination of spectrometers enabled the on-flow collection of full UV, (1)H NMR, IR and mass spectra for a range of common polymer additives in amounts ranging from ca. 230 to 900 micro g on-column. The practical difficulties associated with multiple hyphenation and potential future developments are discussed.
Butylated Hydroxytoluene↗
Flow injection analysis with multiple on-line spectroscopic analysis (UV, IR, 1H-NMR and MS).
Studies on the capabilities of flow injection analysis (FIA) combined with on-line characterisation of model compounds via a combination of diode array UV, 1H-NMR, FT-IR spectroscopy and mass spectrometry are described. Using this combination of spectrometers enabled the on-flow collection of UV, 1H-NMR, IR and MS for a range of model compounds. Samples were introduced into the system as solutions in deuterium oxide in concentrations ranging from 1.4 to 8.4 mg ml(-1). A sample volume of 100 microl was used for FIA at a flow rate of 1 ml min(-1). From these studies a practical working quantity of ca. 140 microg/sample of analyte was determined which provided characteristic spectra.
4-Aminobenzoic Acid↗
HPLC analysis of ecdysteroids in plant extracts using superheated deuterium oxide with multiple on-line spectroscopic analysis (UV, IR, 1H NMR, and MS).
HPLC, using superheated D20 as the mobile phase, combined with on-line characterization via a combination of diode array UV, 1H NMR, FT-IR spectroscopy, and mass spectrometry has been used for the analysis of a standard of 20-hydroxyecdysone- and ecdysteroid-containing plant extracts. This combination of spectrometers enabled the on-flow collection of UV, 1H NMR, IR, and mass spectra not only for pure 20-hydroxyecdysone (100-400 microg on column) but also the major ecdysteroids present in crude extracts of Silene otites, Silene nutans, and Silene frivaldiskyana. The ecdysteroids unequivocally identified in these extracts included 20-hydroxyecdysone, polypodine B, and integristerone A.
Animals↗
Src and Wnt converge to seal cell's fate.
Src family kinases (SFKs) play many roles in the development and growth of flies and mice. In the July, 2002 issue of Developmental Cell, Bei et al. show that a C. elegans SFK collaborates with the Wnt pathway to specify cell fate in early development.
Animals↗
Combinatorial labelling of DNA probes enables multicolour fluorescence in situ hybridisation in plants.
This paper demonstrates a simple but effective use of combinatorial probes to label plant chromosomes by multicolour fluorescence in situ hybridisation (FISH). Three different DNA probes were labelled with only two different fluorophores, hybridised to somatic metaphase chromosomes of Secale cereale and Triticum aestivum, simultaneously visualised, and unequivocally distinguished in a single FISH experiment. Combinatorial labelling can augment karyotypical investigations, physical mapping of chromosomes and other analyses in plants based upon FISH.
Chromosomes↗
Controversies in carotid stenting.
Endovascular therapies are now commonly used in many vascular sites. However, the role for carotid angioplasty and stenting (CAS) remains an unproven therapy with some potential benefits. Initial results of CAS were worse than the surgical standard of carotid endarterectomy (CEA) and did not meet American Heart Association guidelines. However, recent improvements have resulted in improved stroke morbidity rate that may approach that of CEA. Specifically, the embolic problem associated with CAS has been reduced with embolic protection devices, but the ultimate effect of these protection devices remains uncertain. Initial comparison studies of CAS to CEA showed an unacceptably high stroke morbidity rate in the CAS group. As a result, multiple randomized clinical trials have been initiated to compare the results of CAS with embolic protection to that of CEA. While these studies are underway, the authors advocate a careful application of CAS to be used as a complementary tool for the carotid surgeon to use in special circumstances when CEA cannot be undertaken with acceptable morbidity. Furthermore, since the reported stroke morbidity rate of CAS exceeds the AHA recommendation for treatment of asymptomatic patients, most nonsurgical patients with asymptomatic disease should be treated with medical therapy.
Angioplasty↗