Biomedical subjects

Steven M Colegate

Publications and source records attributed to Steven M Colegate.

3 recordsLinked to original sources

PubMed2004

Solid-phase extraction and LC-MS analysis of pyrrolizidine alkaloids in honeys.

Strong-cation-exchange, solid-phase extraction of pyrrolizidine alkaloids and their N-oxides from honey samples was followed by reduction of the N-oxides and subsequent analysis of total pyrrolizidine alkaloids using high-performance liquid chromatography-atmospheric pressure chemical ionization mass spectrometry. A limited survey of 63 preprocessing samples of honey, purposefully biased toward honeys attributed to floral sources known to produce pyrrolizidine alkaloids, demonstrated levels of pyrrolizidine alkaloids up to approximately 2000 parts per billion (ppb) in a sample attributed to Echium plantagineum. Up to 800 ppb pyrrolizidine alkaloids was detected in some honeys not attributed by the collector to any pyrrolizidine alkaloid-producing floral source. No pyrrolizidine alkaloids were detected in approximately 30% of the samples in this limited study, while some honeys showed the copresence of pyrrolizidine alkaloids from multiple floral sources such as E. plantagineum and Heliotropium europaeum. In addition, retail samples of blended honeys (with no labeling to suggest that pyrrolizidine alkaloid-producing floral sources were used in the blends) have been shown to contain up to approximately 250 ppb pyrrolizidine alkaloids.

Chromatography, High Pressure Liquid↗

PubMed2002

Polyhydroxy alkaloids: chromatographic analysis.

Polyhydroxy alkaloids are a burgeoning category of natural products that encompass several structural types and generally exhibit potent activity as inhibitors of glycosidases. As presently defined the group consists of monocyclic or bicyclic aLkaloids of the pyrrolidine, piperidine, pyrrolizidine, indolizidine and tropane classes, bearing two or more hydroxyl groups. These structural features render the compounds highly water soluble and frequently quite insoluble in non-hydroxylic solvents, so that their isolation and analysis by chromatographic means are consequently difficult. This problem is further confounded by the lack of a chromophore which would permit their detection by UV absorption. This review presents chromatographic techniques that have been successfully applied to the problem of isolating, purifying, detecting and analyzing polyhydroxy alkaloids.

Alkaloids↗

PubMed2002

Toxic effects of a single parenteral dose of tunicamycin in late stage pregnancy in rats.

Tunicamycin (TM) was given as a single parenteral dose at 3 levels to female rats at gestation day 15 (GD 15) and also to non-pregnant rats. At 16 h post-dosing all pregnant rats had moderate to extensive vaginal bleeding and 1/4 at each of the 2 higher doses died. The other severely affected rats, euthanized after 26-28 h, had free blood in the uterus and large decreases in red cell count (RCC), hemoglobin (Hb) and packed cell volume (PCV). The amnion of the fetuses was very easily detached from the maternal placenta by gentle manipulation. Histologically, hemorrhage, venous thrombosis and ischemic necrosis, particularly in the maternal placenta, were consistent with the gross appearance. There was no hemorrhage in any control pregnant rats. In the remaining TM-treated pregnant rats, euthanized at GD 17, there were lesser dose-related decreases in RCC, Hb and PCV, but there was no evidence of bleeding or changes in red blood cell parameters in non-pregnant rats. There was a dose-related decrease in cholesterol and GlcNAc-1-P transferase (GPT) activity and a treatment-related decrease in serum proteins in all rats. Maternal toxicity was demonstrated in pregnant rats at TM doses < 10% of a TM lethal dose in non-pregnant rats.

Animals↗