PubMed Health⌕ Search

Biomedical subjects

Sug-Joon Ahn

Publications and source records attributed to Sug-Joon Ahn.

12 recordsLinked to original sources
PubMed2006

Involvement of oxidative stress in mutagenicity and apoptosis caused by dental resin monomers in cell cultures.

OBJECTIVE: This investigation studied the possibility that apoptosis as well as mutagenicity induced by resin monomers are mediated by oxidative stress. METHODS: A range of dilutions of three resin monomers (GMA, TEGDMA, and HEMA) was added to culture medium (DMEM/10% FBS), of V79-4 fibroblasts and RPC-C2A pulp cells for 24 h. Their cytotoxic effects were measured by a colorimetric functional assay (MTT). Chromosomal aberration induced by the resin monomers was investigated by counting micronuclei in V79-4 cells. The effects of the resin monomers on DNA fragmentation were viewed by agarose gel electrophoresis of DNA, isolated from RPC-C2A pulp cells that were treated by resin compounds. Resin monomer-induced apoptosis was further confirmed by flow cytometry (staining with both annexin V-FITC and PI). RESULTS: All monomers exhibited a dose-dependent cytotoxic effect, and the ranking of the cytotoxicity based on TC50 was GMA > TEGDMA > HEMA. The resin monomer-induced cytotoxicity was significantly decreased by co-treatment with N-acetylcystein (NAC), an antioxidant. The authors also confirmed a dose-dependent genotoxicity of the resin monomers that had induced micronucleated cells in V79-4 fibroblasts. Similar to the effects on cytotoxicity, NAC reduced the numbers of micronuclei in comparison with those generated by the resin monomers. The preventive effects of NAC were also observed in monomer-induced apoptosis in RPC-C2A cells. A DNA ladder pattern, characteristic of apoptosis, was shown at cytotoxic concentrations, but NAC blocked the resin monomer-mediated DNA fragmentation. The preventive effects of NAC on apoptosis were confirmed by Annexin V staining. Cells exposed to 300 microM GMA, 7 mM TEGDMA, or 14 mM HEMA for 24 h showed a significant increase in apoptotic cells, while NAC co-treatment caused a reduction in apoptotic cells compared to controls. SIGNIFICANCE: These findings suggest that glutathione depletion and oxidative stress are responsible for GMA, TEGDMA, and HEMA-induced mutagenicity and apoptosis.

Animals↗
PubMed2006

Discrimination of internal derangement of temporomandibular joint by lateral cephalometric analysis.

INTRODUCTION: The purposes of this study were to analyze the progress of internal derangement (ID) of the temporomandibular joint (TMJ) and to find critical yardsticks that can be used during lateral cephalometric analysis to identify subjects with potential ID. METHODS: The sample consisted of 134 women whose primary complaints were malocclusions. They were divided into 5 groups based on the results of magnetic resonance imaging of bilateral TMJs: bilateral normal disc position, unilateral disc displacement with reduction (DDR) and contralateral normal disc position, bilateral DDR, unilateral DDR and contralateral disc displacement without reduction (DDNR), and bilateral DDNR. Thirty-six cephalometric variables from their lateral cephalograms were analyzed with 1-way ANOVA and discriminant analysis to determine the key factors in identifying subjects with TMJ ID. RESULTS: Backward positioning of the mandible, clockwise rotation of the mandible, proclination of the mandibular incisors, and increase in overjet intensified gradually with the progression of TMJ ID, and the subjects with bilateral DDNR showed the greatest changes in dentofacial morphology. Stepwise variable selection in discriminant analysis identified the following 2 variables: mandibular incisor to Frankfort horizontal plane angle and overjet. Discriminant analysis resulted in the correct classification of 79.1% of the subjects and showed that those with smaller mandibular incisor to Frankfort horizontal plane angles and larger overjets had high possibilities of TMJ ID. CONCLUSIONS: This study suggests that some cephalometric variables can be used as an auxiliary diagnostic tool to help identify patients with potential TMJ ID.

Adolescent↗
PubMed2006

Evaluation of internal derangement of the temporomandibular joint by panoramic radiographs compared with magnetic resonance imaging.

INTRODUCTION: Panoramic radiographs are routinely used in orthodontic diagnosis and treatment planning. It would be useful if these radiographs could also provide information about internal derangement (ID) of the temporomandibular joint (TMJ). METHODS: The sample consisted of 168 condyles in 84 women. They underwent panoramic radiography on 1 of 3 machines and bilateral magnetic resonance imaging of the TMJ on 1 machine. Six panoramic variables were analyzed by 2-factor ANOVA to evaluate differences in condylar morphology with respect to status of the TMJ ID and type of panoramic machine. RESULTS: Decreased condylar heights and distally inclined condyles were found in the patients with TMJ ID, irrespective of the type of panoramic machine used. The change became more severe as ID progressed to disk displacement without reduction. CONCLUSIONS: This study suggests that some panoramic variables can help the clinician identify patients with potential ID.

Adolescent↗
PubMed2006

Quantitative determination of adhesion patterns of cariogenic streptococci to various orthodontic adhesives.

OBJECTIVE: To investigate the adhesion of various cariogenic streptococci to orthodontic adhesives. MATERIALS AND METHODS: Five light-cure orthodontic adhesives (one fluoride-releasing composite, three non-fluoride-releasing composites, and one resin-modified glass ionomer cement) were used. The adhesive type, bacterial strain, incubation time, and saliva coating were studied. Thirty specimens of each adhesive were incubated with unstimulated whole saliva or phosphate-buffered saline for 2 hours. Binding assays were then performed by incubating tritium-labeled streptococci with the adhesives for 3 or 6 hours. RESULTS: The results showed a characteristic adhesion pattern according to the type of bacterial strains used. Streptococcus mutans LM7 showed the highest amount of adhesion, whereas S sobrinus B13 showed the lowest amount of adhesion. The cariogenic streptococci adhered to the glass ionomer significantly more than to the composites, whereas there was no significant difference in the adhesion amount among the four composites. The extended incubation time significantly increased bacterial adhesion. However, saliva coating did not significantly alter adhesion patterns of cariogenic streptococci. CONCLUSIONS: This study suggests that cariogenic streptococci can adhere diversely according to adhesive type and that the adhesion of the cariogenic streptococci is not influenced by its fluoride-releasing properties.

Acrylic Resins↗
PubMed2005

Relationship between internal derangement of the temporomandibular joint and dentofacial morphology in women with anterior open bite.

INTRODUCTION: Anterior open bite is known to be associated with internal derangement of the temporomandibular joint (TMJ). This study examined the relationships between internal derangement and dentofacial morphology in women with anterior open bite. METHODS: Fifty-one women with anterior open bite were enrolled in this study. The sample was divided into 3 groups based on magnetic resonance imaging of bilateral TMJs: normal disk position, disk displacement with reduction, and disk displacement without reduction. One-way analysis of variance was used to compare the 3 groups with respect to the cephalometric variables, and Duncan's multiple comparisons were performed at the 95% confidence level to identify the differences among the 3 groups. RESULTS: Internal derangement of the TMJ was much more prevalent in subjects with a more posteriorly rotated mandibular ramus, a smaller mandible, and a greater tendency for a skeletal Class II pattern, although all subjects had an anterior open bite. These patterns were more severe as the internal derangement progressed to disk displacement without reduction. CONCLUSIONS: Some cephalometric characteristics, such as a decrease in posterior facial height, decrease in ramus height, and backward rotation and retruded position of the mandible, are associated with TMJ internal derangement in women with anterior open bite.

Adult↗
PubMed2005

Histomorphometric and mechanical analyses of the drill-free screw as orthodontic anchorage.

INTRODUCTION: Drill-free screws were developed to provide convenient orthodontic anchorage. The purpose of this study was to evaluate the effects of the drilling procedure on the stability of the screws under early orthodontic loading. METHODS: Thirty-two screws were inserted into the jaws of 2 beagles. The screws were divided into 2 groups of 16: the drilling group and the drill-free group. Screws in the drilling group were inserted into the site that had been drilled with a pilot drilling bur, and those in the drill-free group were inserted without drilling. A force of 200 to 300 g was applied using nickel-titanium coil springs 1 week after insertion. Twelve weeks after insertion, mobility was tested with Periotest (Siemens AG, Bensheim, Germany), and the screws with the surrounding bone were prepared for histomorphometric evaluation. RESULTS: Screws in the drill-free group showed less mobility and more bone-to-metal contact; they had more bone area compared with the drilling group, although bone osseointegration was generally found in both groups. CONCLUSIONS: With careful technique, drill-free screws can provide stable orthodontic anchorage.

Animals↗
PubMed2005

Relationship between temporomandibular joint internal derangement and facial asymmetry in women.

INTRODUCTION: Internal derangement (ID) of the temporomandibular joint (TMJ) can cause facial asymmetry. The purposes of this study were to analyze the relationship between facial asymmetry and TMJ ID by using posteroanterior cephalometric variables, and to compare the findings with the results of magnetic resonance imaging (MRI). METHODS: The sample consisted of women seeking orthodontic treatment at Seoul National University Dental Hospital who had routine posteroanterior cephalograms and bilateral MRIs of the TMJ. To eliminate the influence of condylar hyperplasia on facial asymmetry, only those with SNB angles less then 78 degrees were selected (n = 63). They were classified into 5 groups according to the results of the MRI: bilateral normal disk position, unilateral normal TMJ and contralateral disk displacement with reduction (DDR), bilateral DDR, unilateral DDR and contralateral disk displacement without reduction (DDNR), and bilateral DDNR. Fourteen variables from posteroanterior cephalograms were analyzed with 1-way ANOVA to evaluate differences among the 5 groups. RESULTS: Subjects with TMJ ID of greater severity on the unilateral side had shorter ramal height compared with those with bilateral normal or bilateral DDR or bilateral DDNR. In addition, the mandibular midpoint deviated toward the side where the TMJ ID was more advanced. CONCLUSIONS: Subjects with a more degenerated TMJ on the unilateral side might have facial asymmetry that does not come from condylar or hemi-mandibular hyperplasia.

Adolescent↗
PubMed2005

Quantitative analysis of the adhesion of cariogenic streptococci to orthodontic metal brackets.

The aim of this study was to analyze the adhesion of cariogenic streptococci to orthodontic metal brackets in terms of the type of bacterial strains, the incubation time, and saliva coating. Two strains of Streptococcus mutans (S. mutans LM7 and S. mutans OMZ65) and two strains of S. sobrinus (S. sobrinus B13 and S. sobrinus 6715) were used. Twenty metal brackets were incubated with either unstimulated whole saliva or phosphate-buffered saline for two hours. The bacterial adhesion assays were then performed by incubating the tritium-labeled streptococci with saliva-coated or noncoated orthodontic brackets for three, six, or nine hours. The results showed a characteristic binding pattern according to the type of bacterial strains used. S. mutans OMZ65 showed the highest amount of adhesion, whereas S. sobrinus B13 showed the lowest amount of adhesion. Generally, an extended incubation time increased the adhesion of cariogenic streptococci, and the amount of adhesion was the highest after nine hours of incubation. The saliva coating did not significantly influence the adhesion of bacteria. However, this saliva-mediated adhesion differed according to incubation time. The saliva coating tended to gradually decrease the adhesion by the extended incubation time, compared with the noncoated controls. This study indicates that each strain of cariogenic streptococci has a characteristic adhesion pattern and the type of bacterial strain, the incubation time, and saliva influenced the adhesion.

Adult↗
PubMed2005

Cephalometric predictors of long-term stability in the early treatment of Class III malocclusion.

The aim of this study was to examine the differences in the early craniofacial morphology of Class Ill malocclusions. Lateral cephalograms of 45 subjects with a Class III malocclusion and an anterior crossbite in the deciduous or mixed dentition were examined before treatment, after treatment, and during the long-term retention stage. The anterior crossbites of all patients were corrected after a series of orthodontic treatments. After a mean follow-up period of 5.7 years, all the subjects were reevaluated and divided into three groups according to the final occlusal status: good, fair, and poor occlusal stability. Twenty cephalometric variables on the pretreatment lateral cephalograms were analyzed by one-way analysis of variance and discriminant analysis to identify the key determinants for discriminating among the three groups. Among the 20 variables, 11 showed statistical significance. Generally, the subjects with a smaller gonial angle and a more hypodivergent skeletal pattern had good prognosis after the early treatment of Class III malocclusion. When the AB to mandibular plane angle and N-perpendicular to point A were selected in discriminant analysis, the AB to mandibular plane angle was the most significant variable. Discriminant analysis showed a relatively high degree of correct classifications of the patients with early Class III malocclusion. In particular, discriminant analysis showed the highest accuracy (93.3%) when predicting a poor prognosis.

Adolescent↗
PubMed2004

Cephalometric keys to internal derangement of temporomandibular joint in women with Class II malocclusions.

The aim of this study was to find cephalometric keys to provide information on the progression of temporomandibular internal derangement. The sample consisted of 58 women with Class II malocclusions. They were examined with routine lateral cephalograms and magnetic resonance imaging of the temporomandibular joint (TMJ) before orthodontic treatment. They were classified into 3 groups according to the results of the magnetic resonance imaging: normal disk position, disk displacement with reduction, and disk displacement without reduction. Thirty-four cephalometric variables regarding their pretreatment lateral cephalograms were analyzed by 1-way analysis of variance to evaluate the differences in the dentofacial morphology among the 3 groups. The results showed a decrease in posterior facial height, a decrease in ramus height, and backward rotation and retruded position of the mandible in the subjects with internal derangement of the TMJ. These changes were less severe in those with disk displacement with reduction and more severe as internal derangement progressed to disk displacement without reduction. The results suggest that internal derangement of the TMJ might induce dentofacial changes, and that some cephalometric variables can assist in identifying potential patients with internal derangement of the TMJ.

Adolescent↗
PubMed2003

Adhesion of oral streptococci to experimental bracket pellicles from glandular saliva.

The aim of this study was to evaluate the functions of bracket pellicles as the binding receptors for Streptococcus mutans and Streptococcus gordonii. Four different types of orthodontic brackets were used: stainless steel, monocrystalline sapphire, polycrystalline alumina, and plastic. The bracket pellicles were formed by incubating orthodontic brackets with fresh submandibular-sublingual saliva or parotid saliva for 2 hours. The pellicles were extracted, and their components were confirmed by gel electrophoresis, immunodetection, and amino acid composition analysis. The roles of the bracket pellicles in the adhesion of oral streptococci were evaluated by incubating tritium-labeled streptococci with pellicle-transfer blots. The results showed that the salivary components adhered selectively according to type of bracket and glandular saliva. The selective adsorption was also proven by the amino acid composition profiles. Among the several salivary proteins, MG2, alpha-amylase, and the acidic proline-rich proteins provided the binding sites for S gordonii. However, none of these proteins in the bracket pellicles contributed to the adhesion of S mutans. These findings suggest that numerous salivary proteins can adhere selectively to the orthodontic brackets, and some of them contribute to the binding of S gordonii.

Adult↗