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Sung-Taik Lee

Publications and source records attributed to Sung-Taik Lee.

At least 19 recordsLinked to original sources

Paenibacillus ginsengisoli sp. nov., a novel bacterium isolated from soil of a ginseng field in Pocheon Province, South Korea.

A Gram-positive, aerobic or facultative anaerobic, motile, spore-forming bacterial strain, designated Gsoil 1638T, was isolated from a soil sample of a ginseng field in Pocheon province (South Korea), and was characterized taxonomically by using a polyphasic approach. It grew well on nutrient agar medium, utilized a fairly narrow spectrum of carbon sources and tolerated 10% NaCl. The isolate was positive for catalase and oxidase tests but negative for the degradation of macromolecules such as casein, collagen, starch, chitin, CM-cellulose, xylan and DNA. The G + C content of the genomic DNA was 50.7 mol%. The predominant isoprenoid quinone was menaquinone 7 (MK-7). The major fatty acids were anteiso-C15:0 (44%) and C16:0 (25%). Comparative 16S rRNA gene sequence analysis showed that strain Gsoil 1638T fell within the radiation of the cluster comprising Paenibacillus species and joined Paenibacillus anaericanus DSM 15890T with a bootstrap value of 100%. These two strains shared 99.5% 16S rRNA gene sequence similarity with each other. The phylogenetic distance from any other validly described species within the genus Paenibacillus was less than 96.2%. DNA-DNA relatedness value between strain Gsoil 1638T and its closest phylogenetic neighbor, Paenibacillus anaericanus, was 62%. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain Gsoil 1638T (= KCTC 13931T = LMG 23406T = CCUG 52472T) was classified in the genus Paenibacillus as the type strain of a novel species, for which the name Paenibacillus ginsengisoli sp. nov. is proposed.

Aerobiosis↗

Chryseobacterium caeni sp. nov., isolated from bioreactor sludge.

A Gram-negative, non-spore-forming, yellow-pigmented bacterium, strain N4(T), was isolated from a nickel-complexed cyanide-degrading bioreactor and subjected to a polyphasic taxonomic study. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain N4(T) is affiliated to the genus Chryseobacterium of the family Flavobacteriaceae. The levels of 16S rRNA gene sequence similarity between strain N4(T) and the type strains of all known Chryseobacterium species were 93.2-95.8 %, suggesting that strain N4(T) represents a novel species within the genus Chryseobacterium. The strain contained iso-C(15 : 0) and summed feature 4 as the major fatty acids and menaquinone MK-6 as the predominant respiratory quinone. The G+C content of the genomic DNA was 38.2 mol%. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain N4(T) represents a novel species of the genus Chryseobacterium, for which the name Chryseobacterium caeni sp. nov. is proposed. The type strain is N4(T) (=KCTC 12506(T)=CCBAU 10201(T)=DSM 17710(T)).

Bacterial Typing Techniques↗

Pleomorphomonas koreensis sp. nov., a nitrogen-fixing species in the order Rhizobiales.

A Gram-negative, non-motile, non-spore-forming, rod-shaped bacterium (strain Y9(T)) was isolated from a contaminated culture of the phototrophic bacterium Rhodopseudomonas palustris, and was investigated using a polyphasic taxonomic approach. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain Y9(T) belonged to the order Rhizobiales in the Alphaproteobacteria. Comparison of phylogenetic data indicated that it was most closely related to Pleomorphomonas oryzae (98.5 % similarity of 16S rRNA gene sequence), and the phylogenetic distance from any other species of the order Rhizobiales with a validly published name was greater than 7.5 % (i.e. less than 92.5 % similarity). The predominant ubiquinone was Q-10 and the major fatty acids were C(18 : 1), C(16 : 0), C(19 : 0) cyclo omega8c and C(18 : 0). The G+C content of genomic DNA of strain Y9(T) was 65.1 mol%. The results of DNA-DNA hybridization in combination with chemotaxonomic and physiological data demonstrated that strain Y9(T) represents a novel species within the genus Pleomorphomonas, for which the name Pleomorphomonas koreensis sp. nov. is proposed. The type strain is Y9(T) (=KCTC 12246(T)=NBRC 100803(T)).

Alphaproteobacteria↗

Stenotrophomonas koreensis sp. nov., isolated from compost in South Korea.

A Gram-negative, rod-shaped, non-spore-forming bacterium (TR6-01T) was isolated from compost near Daejeon city in South Korea. On the basis of 16S rRNA gene sequence similarity, strain TR6-01T was shown to belong to the class Gammaproteobacteria, related to Stenotrophomonas acidaminiphila (97.1 %) and Stenotrophomonas maltophilia (96.9 %); the phylogenetic distance from any other established species within the genus Stenotrophomonas was less than 97.0 %. Phenotypic and chemotaxonomic data (major ubiquinone Q-8; fatty acid profile) supported the affiliation of strain TR6-01T to the genus Stenotrophomonas. The results of DNA-DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain TR6-01T from the five Stenotrophomonas species with validly published names. TR6-01T therefore represents a novel species, for which the name Stenotrophomonas koreensis sp. nov. is proposed, with the type strain TR6-01T (= KCTC 12211T = JCM 13256T).

Fatty Acids↗

Sphingomonas soli sp. nov., a beta-glucosidase-producing bacterium in the family Sphingomonadaceae in the alpha-4 subgroup of the Proteobacteria.

Strain T5-04T, a Gram-negative, non-spore-forming, rod-shaped bacterium, was isolated from soil of a ginseng field in South Korea and characterized in order to determine its taxonomic position. 16S rRNA gene sequence analysis revealed that strain T5-04T belongs to the alpha-4 subgroup of the Proteobacteria, and the highest degrees of sequence similarity determined were to Sphingomonas asaccharolytica IFO 10564T (97.5 %), Sphingomonas koreensis JSS26T (97.1 %), Sphingomonas mali IFO 15500T (96.7 %) and Sphingomonas pruni IFO 15498(T) (96.6 %). Chemotaxonomic data revealed that strain T5-04T possesses ubiquinone Q-10 predominantly, C(18 : 1) as the predominant fatty acid and sphingoglycolipids, all of which corroborate its assignment to the genus Sphingomonas. The results of DNA-DNA hybridization and physiological and biochemical tests clearly demonstrated that strain T5-04T represents a distinct species. Based on polyphasic evidence, T5-04T (=KCTC 12210T = NBRC 100801T = IAM 15213T) should be classified as the type strain of a novel Sphingomonas species, for which the name Sphingomonas soli sp. nov. is proposed.

Molecular Sequence Data↗

Shinella granuli gen. nov., sp. nov., and proposal of the reclassification of Zoogloea ramigera ATCC 19623 as Shinella zoogloeoides sp. nov.

The taxonomic position of a novel bacterial strain, Ch06T, isolated from an upflow anaerobic sludge blanket reactor was determined. Strain Ch06T was Gram-negative, aerobic, motile and oxidase- and catalase-positive. A comparative 16S rRNA gene sequence analysis showed a clear affiliation of strain Ch06T to the Alphaproteobacteria and it was most closely related to Zoogloea ramigera ATCC 19623 and Mycoplana dimorpha IAM 13154T (97.9 and 96.3% sequence similarity, respectively). The major respiratory quinone was Q-10 and the predominant fatty acids were C16:0, 3-OH C16:0, C18:0, C19:0 cyclo omega8c and summed feature 7 (C18:1omega7c/omega9t/omega12t, C18:1omega7c/omega9c/omega12t). On the basis of phenotypic, chemotaxonomic and phylogenetic characteristics, the novel isolate was assigned to a new genus, Shinella gen. nov., as Shinella granuli gen. nov., sp. nov. (type strain Ch06T=KCTC 12237T=JCM 13254T). It is proposed that Zoogloea ramigera ATCC 19623 is reclassified into the novel genus Shinella as Shinella zoogloeoides sp. nov. (type strain ATCC 19623T=IAM 12669T=I-16-MT).

Alphaproteobacteria↗

Microbacterium paludicola sp. nov., a novel xylanolytic bacterium isolated from swamp forest.

A xylanolytic bacterium, US15T, was isolated from swamp forest soil in Ulsan, Korea. The cells of the novel strain were Gram-positive, non-motile, short-rod-shaped and showed chemotaxonomic properties that were consistent with its classification in the genus Microbacterium. Chemotaxonomic results showed MK-12 and MK-11 as major menaquinones, predominating iso- and anteiso-branched cellular fatty acids, glucose, galactose and mannose as cell-wall sugars, peptidoglycan-type B2beta with glycolyl residues and a DNA G + C content of 66.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequencing showed that strain US15T was closely related to Microbacterium arborescens IFO 3750T, Microbacterium imperiale IFO 12610T and Microbacterium ulmi LMG 20991T (96.9, 96.8 and 96.2 % similarities, respectively), and formed a separate lineage within the genus Microbacterium. Combined genotypic and phenotypic data showed that strain US15T (= DSM 16915T= KCTC 19080T) merits recognition as the type strain of a novel species within the genus Microbacterium, for which the name Microbacterium paludicola sp. nov. is proposed.

Actinomycetales↗

Burkholderia terrae sp. nov., isolated from a forest soil.

A Gram-negative, slightly curved rod-shaped bacterium, designated strain KMY02T, was isolated from a forest soil in Daejeon, South Korea. On the basis of 16S rRNA gene sequence similarity, strain KMY02T was shown to belong to the family Burkholderiaceae of the Betaproteobacteria, and to be related most closely to Burkholderia hospita LMG 20598T (98.7%), Burkholderia caribensis LMG 18531T (98.0%) and Burkholderia phymatum LMG 21445T (97.4%). Its phylogenetic distance from all recognized species within the genus Burkholderia was less than 97%. Chemotaxonomic data [Q-8 as the major ubiquinone; C16:0, C17:0 cyclo, summed feature 7 (C18:1omega7c/omega9t/omega12t) and C15:0 as the major fatty acids] supported the affiliation of strain KMY02T to the genus Burkholderia. The results of DNA-DNA hybridization experiments and physiological and biochemical tests allowed genotypic and phenotypic differentiation of the strain from recognized Burkholderia species. Therefore, KMY02T (=KCTC 12388T=NBRC 100964T) represents the type strain of a novel species, for which the name Burkholderia terrae sp. nov. is proposed.

Bacterial Typing Techniques↗

Azonexus caeni sp. nov., a denitrifying bacterium isolated from sludge of a wastewater treatment plant.

A polyphasic taxonomic study was carried out to determine the taxonomic position of a newly isolated denitrifying bacterium, designated Slu-05T, which had been isolated from sludge from the main aerobic treatment tanks of a municipal sewage treatment plant. Phylogenetic analysis based on comparative 16S rRNA gene sequencing indicated that strain Slu-05T was closely related to Azonexus fungiphilus LMG 19178T (96.4 % sequence similarity), the sole species in the genus Azonexus. Strain Slu-05T comprised Gram-negative, motile, non-spore-forming and slightly curved rods. The predominant respiratory lipoquinone was Q-8. The major fatty acids were C16:1omega7c, C16:0, C18:1 isomers and C10:0 3-OH. The G+C content of the genomic DNA was 65.6 mol%. The results of DNA-DNA hybridization (15.6 %) together with phenotypic determination showed that strain Slu-5T could be distinguished from A. fungiphilus. Moreover, some phenotypic properties concerning enzyme activity, the substrates utilized as carbon sources and growth conditions distinguish strain Slu-5T from A. fungiphilus. On the basis of the results obtained in this study, Slu-05T (=DSM 17719T=KCTC 12530T=CCBAU 10199T) is the type strain of a novel species of Azonexus, for which the name Azonexus caeni sp. nov. is proposed.

Bacterial Typing Techniques↗

Starkeya koreensis sp. nov., isolated from rice straw.

A facultatively chemolithoautotrophic, Gram-negative, aerobic, highly curved rod-shaped, non-motile, non-spore-forming bacterium, strain Jip08(T), was isolated from rice straw in Daejeon, South Korea. Strain Jip08(T) produced colourless colonies (1.0-1.5 mm) on R2A medium after 3 days. On the basis of 16S rRNA gene sequence similarity, strain Jip08(T) was shown to belong to the alpha-2 subclass of the Proteobacteria, and was most closely related to Starkeya novella IAM 12100(T) (98.6 %), Angulomicrobium tetraedale DSM 5895(T) (98.1 %), Angulomicrobium amanitiforme NCIMB 1785(T) (98.0 %), Ancylobacter aquaticus ATCC 25396(T) (96.6 %) and Methylorhabdus multivorans DM13(T) (95.8 %), all of which belong to the family Hyphomicrobiaceae. The genomic DNA G+C content of strain Jip08(T) was 69 mol%. Chemotaxonomic data [major ubiquinone, Q-10; major fatty acids, C(18 : 1) isomer (summed feature of C(18 : 1)omega7c/omega9t/omega12t), C(19 : 0) cyclo omega8c and C(16 : 0)], 16S rRNA gene sequence analyses and some morphological and physiological features supported the affiliation of strain Jip08(T) to the genus Starkeya. However, DNA-DNA hybridization data and some phenotypic properties showed that strain Jip08(T) could be distinguished from the single known Starkeya species and represented a novel species, for which the name Starkeya koreensis sp. nov. is proposed. The type strain is Jip08(T) (=KCTC 12212(T)=NBRC 100963(T)=IAM 15215(T)).

Alphaproteobacteria↗

Lactobacillus siliginis sp. nov., isolated from wheat sourdough in South Korea.

The taxonomic position of two lactic-acid-producing bacterial strains, isolated from wheat sourdough in South Korea, was studied using a polyphasic approach. Phylogenetic analysis on the basis of 16S rRNA gene sequences and biochemical and physiological characteristics indicated these two strains to be members of the genus Lactobacillus. They had high 16S rRNA gene sequence similarity (98.5 %) with Lactobacillus rossiae DSM 15814(T) and very low (<94.0 %) similarity with any other recognized species of the genus Lactobacillus. These two strains (designated M1-212(T) and M2-236) were heterofermentative, facultatively anaerobic, Gram-positive, non-spore-forming, non-motile, short rod-shaped bacteria. The optimum growth temperature for these strains was 30 degrees C (no growth at 15 or 45 degrees C) and they were able to tolerate 5 % (w/v) NaCl. The G+C content of the genomic DNA of the two strains was 45.5 mol%, within the range of values reported for the genus Lactobacillus (32-53 mol%). The peptidoglycan was of the A3alpha (l-lys-d-glu-l-Ala) type. Physiological, biochemical and genotypic data, as well as results of DNA-DNA hybridization of the genomic DNA with one of the closest phylogenetic relatives, L. rossiae DSM 15814(T), indicated that the strains represent a novel species of the genus Lactobacillus, for which the name Lactobacillus siliginis sp. nov. is proposed. The type strain is M1-212(T) (=KCTC 3985(T)=NBRC 101315(T)).

Base Composition↗

Dyadobacter ginsengisoli sp. nov., isolated from soil of a ginseng field.

A Gram-negative, aerobic, non-motile, non-spore-forming and rod-shaped bacterium, strain Gsoil 043(T), was isolated from soil from a ginseng field in Pocheon province, South Korea. The novel isolate was characterized in order to determine its taxonomic position. On the basis of 16S rRNA gene sequence similarity, strain Gsoil 043(T) was shown to belong to the family 'Flexibacteraceae' and was related to Dyadobacter fermentans (96.7 %), Dyadobacter crusticola (96.3 %) and Dyadobacter hamtensis (95.8 %). The 16S rRNA gene sequence similarity of the novel strain to other recognized species within the family 'Flexibacteraceae' was less than 87.0 %. The G+C content of genomic DNA was 48 mol%. Phenotypic and chemotaxonomic data (major menaquinone, MK-7; major fatty acids, C(16 : 1)omega7c, iso-C(15 : 0) and C(16 : 0)) supported the affiliation of strain Gsoil 043(T) to the genus Dyadobacter. The results of physiological and biochemical tests enabled strain Gsoil 043(T) to be differentiated genotypically and phenotypically from the three Dyadobacter species with validly published names. The novel isolate therefore represents a novel species for which the name Dyadobacter ginsengisoli sp. nov. is proposed, with the type strain Gsoil 043(T) (=KCTC 12589(T)=LMG 23409(T)).

Bacteroidetes↗

Brevibacillus ginsengisoli sp. nov., a denitrifying bacterium isolated from soil of a ginseng field.

A Gram-positive, rod-shaped, spore-forming bacterium, Gsoil 3088T, was isolated from soil from a ginseng field in Pocheon Province in South Korea and characterized in order to determine its taxonomic position. On the basis of 16S rRNA gene sequence similarity, strain Gsoil 3088T was shown to belong to the family Paenibacillaceae, being related to Brevibacillus centrosporus (96.6%), Brevibacillus borstelensis (96.3%), Brevibacillus parabrevis (96.1%), Brevibacillus formosus (96.1%), Brevibacillus brevis (96.1%) and Brevibacillus laterosporus (96.0%). The phylogenetic distances from other validly described species within the genus Brevibacillus were greater than 4.0% (i.e. there was less than 96.0% similarity). The G+C content of the genomic DNA was 52.1 mol%. Phenotypic and chemotaxonomic data (major menaquinone, MK-7; fatty acid profile, iso-C15:0, iso-C14:0 and anteiso-C15:0) supported the affiliation of strain Gsoil 3088T to the genus Brevibacillus. The results of physiological and biochemical tests allowed strain Gsoil 3088T to be distinguished genotypically and phenotypically from Brevibacillus species with validly published names. Strain Gsoil 3088T, therefore, represents a novel species of the genus Brevibacillus, for which the name Brevibacillus ginsengisoli sp. nov. is proposed. The type strain is Gsoil 3088T (=KCTC 13938T=LMG 23403T).

Bacterial Typing Techniques↗

Bacillus panaciterrae sp. nov., isolated from soil of a ginseng field.

A Gram-positive, non-motile, endospore-forming bacterium, designated Gsoil 1517(T), was isolated from soil of a ginseng field in Pocheon Province (South Korea) and was characterized in order to determine its taxonomic position, using a polyphasic approach. It was found to rod-shaped and aerobic or facultatively anaerobic. It grew optimally at 30 degrees C and at pH 6.5-7.0. Comparative 16S rRNA gene sequence analysis showed that strain Gsoil 1517(T) forms a distinct phylogenetic lineage within the genus Bacillus, being related to Bacillus funiculus JCM 11201(T) (96.8 %). The strain showed less than 94.3 % sequence similarity with other Bacillus species. The G+C content of the genomic DNA was found to be 47.8 mol% and the predominant respiratory quinone was MK-7. The major fatty acids were iso-C(15 : 0) (42.4 %), anteiso-C(15 : 0) (17.4 %), iso-C(14 : 0) (9.7 %) and C(16 : 0) (6.0 %). On the basis of its phenotypic properties and phylogenetic distinctiveness, strain Gsoil 1517(T) represents a novel species of the genus Bacillus, for which the name Bacillus panaciterrae sp. nov. is proposed. The type strain is Gsoil 1517(T) (=KCTC 13929(T)=CCUG 52470(T)=LMG 23408(T)).

Agriculture↗

Paenibacillus panacisoli sp. nov., a xylanolytic bacterium isolated from soil in a ginseng field in South Korea.

A Gram-positive, facultatively anaerobic, motile, spore-forming bacterium, designated Gsoil 1411T, was isolated from soil of a ginseng field in Pocheon Province (South Korea) and was characterized using a polyphasic approach. Comparative analysis of 16S rRNA gene sequences revealed that strain Gsoil 1411T belongs to the family Paenibacillaceae, with closest sequence similarity to the type strains of Paenibacillus xylanilyticus (95.7%), Paenibacillus illinoisensis (95.2%) and Paenibacillus pabuli (94.8%). Strain Gsoil 1411T showed less than 94% sequence similarity to the type strains of other recognized members of the genus Paenibacillus. In addition, the presence of MK-7 as the major menaquinone, anteiso-C15:0 as a major fatty acid (44.8%) and the presence of PAEN513F and PAEN862F signature sequences suggest that it is affiliated to the genus Paenibacillus. The G+C content of the genomic DNA was 53.9 mol%. On the basis of its phenotypic characteristics and phylogenetic distinctiveness, strain Gsoil 1411T is suggested to represent a novel species within the genus Paenibacillus, for which the name Paenibacillus panacisoli sp. nov. is proposed. The type strain is Gsoil 1411T (=KCTC 13020T=LMG 23405T).

Bacterial Typing Techniques↗

Sphingobacterium daejeonense sp. nov., isolated from a compost sample.

A Gram-negative, strictly aerobic, rod-shaped, non-motile, non-spore-forming bacterial strain, designated TR6-04(T), was isolated from compost and characterized taxonomically by using a polyphasic approach. The organism grew optimally at 30 degrees Celsius and at pH 6.5-7.0. The isolate was positive for catalase and oxidase tests but negative for gelatinase, indole and H(2)S production. Comparative 16S rRNA gene sequence analysis showed that strain TR6-04(T) fell within the radiation of the cluster comprising Sphingobacterium species and clustered with Sphingobacterium mizutaii ATCC 33299(T) (96.7 % sequence similarity); the similarity to sequences of other species within the family Sphingobacteriaceae was less than 92.0 %. The G+C content of the genomic DNA was 38.7 mol%. The predominant respiratory quinone was MK-7. The major fatty acids were iso-C(15 : 0), iso-C(17 : 0) 3-OH and summed feature 4 (iso-C(15 : 0) 2-OH and/or C(16 : 1)omega7c). These chemotaxonomic data supported the affiliation of strain TR6-04(T) to the genus Sphingobacterium. However, on the basis of its phenotypic properties and phylogenetic distinctiveness, strain TR6-04(T) (=KCTC 12579(T)=LMG 23402(T)=CCUG 52468(T)) should be classified as the type strain of a novel species, for which the name Sphingobacterium daejeonense sp. nov. is proposed.

Bacterial Typing Techniques↗

Pedobacter ginsengisoli sp. nov., a DNase-producing bacterium isolated from soil of a ginseng field in South Korea.

A Gram-negative, strictly aerobic, rod-shaped, non-motile, non-spore-forming bacterial strain, designated Gsoil 104T, was isolated from a soil sample from a ginseng field in Pocheon Province (South Korea) and was characterized taxonomically by using a polyphasic approach. On the basis of 16S rRNA gene sequence similarities, strain Gsoil 104T was shown to belong to the family Sphingobacteriaceae, being related to Pedobacter africanus DSM 12126T (97.0%), Pedobacter caeni LMG 22862T (96.9%), Pedobacter cryoconitis DSM 14825T (96.8%) and Pedobacter heparinus DSM 2366T (96.6%). The phylogenetic distance from any other Pedobacter species with a validly published name was greater than 3.4% (i.e.<96.6% 16S rRNA gene sequence similarity). DNA-DNA hybridization experiments showed that values for DNA-DNA relatedness between strain Gsoil 104T and its phylogenetically closest neighbours were below 37%. The G+C content of the genomic DNA was 43.6 mol%. The predominant respiratory quinone was MK-7. The major fatty acids were C16:1omega7c, iso-C15:0, C16:0, iso-C17:0 3-OH and iso-C15:0 2-OH. These chemotaxonomic data support the affiliation of strain Gsoil 104T to the genus Pedobacter. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain Gsoil 104T represents a novel species in the genus Pedobacter, for which the name Pedobacter ginsengisoli sp. nov. is proposed. The type strain is Gsoil 104T (=KCTC 12576T=LMG 23399T).

Bacteroidetes↗

Aerobic biodegradation of 4-methylpyridine and 4-ethylpyridine by newly isolated Pseudonocardia sp. strain M43.

A filamentous bacterium capable of utilizing 4-methylpyridine and 4-ethylpyridine as the sole source of carbon, nitrogen and energy was isolated from sludge. The organism, designated as strain M43, clustered most closely with members of the genus Pseudonocardia by 16S rRNA gene sequence analysis. During the degradation of 4-methylpyridine and 4-ethylpyridine, c. 60% of nitrogen in the pyridine ring was released as ammonia. Metabolite analyses showed that 2-hydroxy-4-methylpyridine and 2-hydroxy-4-ethylpyridine were transiently accumulated during the degradation of 4-methylpyridine and 4-ethylpyridine, respectively. Strain M43 was also able to degrade pyridine, 3,4-dimethylpyridine, 4-carboxypyridine and 2-hydroxy-4-methylpyridine. The results indicate that degradation of 4-methylpyridine and 4-ethylpyridine by strain M43 proceeded via initial hydroxylation.

Actinomycetales↗