Biomedical subjects

Susan Murphy

Publications and source records attributed to Susan Murphy.

8 recordsLinked to original sources

PubMed2007

Enantioselectivity of thalidomide serum and tissue concentrations in a rat glioma model and effects of combination treatment with cisplatin and BCNU.

Thalidomide is currently under evaluation as an anti-angiogenic agent in cancer treatment, alone and in combination with cytotoxic agents. Thalidomide is a racemate with known pharmacologic and pharmacokinetic enantioselectivity. In a previous study with thalidomide combination chemotherapy, we found evidence of anti-tumour synergy. In this study, we examined whether the synergy involved altered pharmacokinetics of thalidomide enantiomers. Adult female F344 rats were implanted with 9L gliosarcoma tumours intracranially, subcutaneously (flank), or both. Effectiveness of oral thalidomide alone, and with intraperitoneal BCNU or cisplatin combination chemotherapy, was assessed after several weeks treatment. Presumed pseudo steady-state serum, tumour and other tissues, collected after treatment, were assayed for R- and S-thalidomide by chiral HPLC. Both serum and tissue concentrations of R-thalidomide were 40-50% greater than those of S-thalidomide. Co-administration of BCNU or cisplatin with thalidomide did not alter the concentration enantioselectivity. Poor correlation of concentration with subcutaneous anti-tumour effect was found for individual treatments, and with all treatments for intracranial tumours. The consistency of the enantiomer concentration ratios across treatments strongly suggests that the favourable antitumour outcomes from interactions between thalidomide and the cytotoxic agents BCNU and cisplatin did not have altered enantioselectivity of thalidomide pharmacokinetics as their basis.

Animals↗

PubMed2006

The effects of group exercise on fatigue and quality of life during cancer treatment.

PURPOSE/OBJECTIVES: To determine the feasibility of participating in a structured group exercise program (SGEP) for adult patients receiving cancer treatment and to test the impact of an SGEP on reducing cancer-related fatigue (CRF) and improving quality of life (QOL). DESIGN: One-group, prospective, pre- and post-test design. SETTING: Two community outpatient infusion centers. SAMPLE: Convenience sample of 12 adults with varying cancer diagnoses receiving cancer treatment. METHODS: Nine of 12 subjects participated in SGEP twice weekly for six weeks. Exercises focused on strengthening proximal muscle groups and improving functional ability. All subjects completed the Fatigue Symptom Inventory and the Short Form-36 version 2 (SF-36v2) Health Survey at baseline and six weeks. MAIN RESEARCH VARIABLES: Feasibility of the intervention, CRF, and QOL. FINDINGS: No difference in reported fatigue was found. The SF-36v2 subscale score for bodily pain showed a significant decrease in this symptom. Subscale scores for physical role, vitality, and social function increased but did not yield statistical significance. Social interactions resulted in strong group cohesiveness. A postprogram questionnaire identified themes of support, learning from shared information, and the usefulness of having an exercise program that also serves as an informal support group. CONCLUSIONS: SGEP is feasible, safe, and well tolerated by adult patients with cancer and may have positive effects on CRF and QOL. IMPLICATIONS FOR NURSING: The benefits of exercise for patients with cancer receiving treatment are well documented. Using a multidisciplinary approach, oncology nurses, working with an exercise physiologist, can safely create an SGEP, combining the power of group interactions with the appeal of a wellness-promoting behavior, and provide an additional tool to assist in the management of physiologic and psychosocial effects of cancer treatment.

Adult↗

PubMed2005

Patterns of gene expression that characterize long-term survival in advanced stage serous ovarian cancers.

PURPOSE: A better understanding of the underlying biology of invasive serous ovarian cancer is critical for the development of early detection strategies and new therapeutics. The objective of this study was to define gene expression patterns associated with favorable survival. EXPERIMENTAL DESIGN: RNA from 65 serous ovarian cancers was analyzed using Affymetrix U133A microarrays. This included 54 stage III/IV cases (30 short-term survivors who lived <3 years and 24 long-term survivors who lived >7 years) and 11 stage I/II cases. Genes were screened on the basis of their level of and variability in expression, leaving 7,821 for use in developing a predictive model for survival. A composite predictive model was developed that combines Bayesian classification tree and multivariate discriminant models. Leave-one-out cross-validation was used to select and evaluate models. RESULTS: Patterns of genes were identified that distinguish short-term and long-term ovarian cancer survivors. The expression model developed for advanced stage disease classified all 11 early-stage ovarian cancers as long-term survivors. The MAL gene, which has been shown to confer resistance to cancer therapy, was most highly overexpressed in short-term survivors (3-fold compared with long-term survivors, and 29-fold compared with early-stage cases). These results suggest that gene expression patterns underlie differences in outcome, and an examination of the genes that provide this discrimination reveals that many are implicated in processes that define the malignant phenotype. CONCLUSIONS: Differences in survival of advanced ovarian cancers are reflected by distinct patterns of gene expression. This biological distinction is further emphasized by the finding that early-stage cancers share expression patterns with the advanced stage long-term survivors, suggesting a shared favorable biology.

Case-Control Studies↗

PubMed2005

Challenges in evaluating psychosocial interventions for Autistic Spectrum Disorders.

In 2002, the National Institutes of Health sponsored a meeting concerning methodological challenges of research in psychosocial interventions in Autism Spectrum Disorders. This paper provides a summary of the presentations and the discussions that occurred during this meeting. Recommendations to federal and private agencies included the need for randomized clinical trials of comprehensive interventions for autism as the highest, but not the sole priority. Ongoing working groups were proposed to address psychosocial interventions with a focus on relevant statistics, standardized documentation and methods of diagnosis, development of outcome measures, establishment of standards in research; and the need for innovative treatment designs, including application of designs from other research areas to the study of interventions in ASD.

Autistic Disorder↗

PubMed2005

Transforming growth factor beta receptor I polyalanine repeat polymorphism does not increase ovarian cancer risk.

OBJECTIVES: It has been suggested that the 6A allele of the type I TGFbeta receptor (TGFbetaR1) polyalanine repeat tract polymorphism may increase susceptibility to various types of cancer including ovarian cancer. METHODS: The TGFbetaR1 polyalanine polymorphism was genotyped in 588 ovarian cancer cases and 614 controls from a population-based case-control study in North Carolina. RESULTS: Significant racial differences in the frequency of the 6A allele were observed between Caucasian (10.7%) and African-American (2.4%) controls (P < 0.001). One or two copies of the 6A allele of the TGFbetaR1 polyalanine polymorphism was carried by 18% of all controls and 19% of cases, and there was no association with ovarian cancer risk (OR = 1.07, 95% CI 0.80-1.44). The odds ratio for 6A homozygotes was 1.81 (95% CI 0.655.06), but these comprised only 0.98% of controls and 1.70% of cases. CONCLUSIONS: The 6A allele of the TGFbetaR1 polyalanine polymorphism does not appear to increase ovarian cancer risk. Larger studies would be needed to exclude the possibility that the small fraction of individuals who are 6A homozygotes have an increased risk of ovarian or other cancers.

Adult↗

PubMed2005

Tracking the invasive history of the green alga Codium fragile ssp. tomentosoides.

The spread of nonindigenous species into new habitats is having a drastic effect on natural ecosystems and represents an increasing threat to global biodiversity. In the marine environment, where data on the movement of invasive species is scarce, the spread of alien seaweeds represents a particular problem. We have employed a combination of plastid microsatellite markers and DNA sequence data from three regions of the plastid genome to trace the invasive history of the green alga Codium fragile ssp. tomentosoides. Extremely low levels of genetic variation were detected, with only four haplotypes present in the species' native range in Japan and only two of these found in introduced populations. These invasive populations displayed a high level of geographical structuring of haplotypes, with one haplotype localized in the Mediterranean and the other found in Northwest Atlantic, northern European and South Pacific populations. Consequently, we postulate that there have been at least two separate introductions of C. fragile ssp. tomentosoides from its native range in the North Pacific.

Base Sequence↗

PubMed2003

Recruiting and screening older, transitional to frail adults in congregate living facilities.

Historically older adults have been recruited from the community or from long-term care facilities. The research study, The Effects of Intense Tai Chi Training in the Older Adult, targeted adults 70 years and older who were residing in congregate living facilities and transitioning to frailty according to the Speechley/Tinetti scale. This article describes the strategies that were designed and implemented to recruit and screen the 287 older adults who entered the randomized, single-blinded clinical trial.

Activities of Daily Living↗

PubMedDate not supplied

A theory-based intervention to improve nurses' knowledge, self-efficacy, and skills to reduce PICC occlusion.

The peripherally inserted central catheter (PICC) is an effective intermediate and long-term central vascular access device. Its functions are comparable with those of other percutaneously placed central venous catheters. However, its high occlusion rate and the consequent infection risk may disrupt therapy for patients. The primary investigator in this study developed an educational intervention based on Albert Bandura's social learning theory, and hypothesized that increasing nurses' knowledge and self-efficacy related to PICCs would have a positive impact on patient outcomes related to occlusion and infection rates. The study outcome showed an overall increase in nurses' knowledge and self-efficacy related to PICC care, and a significant reduction in the catheter occlusion rate, from 29% to 8.5%, over a 6-month period.

Attitude of Health Personnel↗