PubMed Health⌕ Search

Biomedical subjects

T A Bonfiglio

Publications and source records attributed to T A Bonfiglio.

At least 55 records · Page 3Linked to original sources
PubMed1976

Isoantigen loss in cervical neoplasia. Demonstration by immunofluorescence and immunoperoxidase techniques.

Tissue sections from normal uterine cervix and from uterine cervices with dysplasia, carcinoma in situ, and invasive cancer were studied in 70 instances using immunofluorescence and immunoperoxidase techniques for demonstrating the presence of isoantigens A and B. Isoantigens were demonstrated in normal squamous epithelium. Antigens were lost in foci of invasive cancer, were considerably reduced or lost in areas of classic carcinoma in situ in all cases, and were reduced in most cases of dysplasia. These observations confirm those obtained with the specific red cell adherence test. The results suggest that immunofluorescence and immunoperoxidase techniques are sensitive, reproducible, easily performed methods for demonstrating the loss of isoantigens in cervical neoplasia.

Carcinoma in Situ↗
PubMed1976

Histopathologic spectrum of benign proliferative and intraepithelial neoplastic reactions of the uterine cervix.

A working model for the histogenesis of carcinoma of the uterine cervix is summarized in Table I. The inclusion of squamous metaplasia in this chart does not imply that this reaction falls into the spectrum of cervical neoplasia or is necessarily an antecedent to neoplasia. It does simply imply that the carcinogenic event or events apparently occur in or involve an epithelium that is indistinguishable from squamous metaplasia. The chart intentionally implies that the lesions mentioned are not separate diseases but arbitrary points in the spectrum of cervical neoplasia. It must be emphasized that one stage does not necessarily progress to the next and that at any stage up to indisputable cancer the changes may regress, persist or progress. The careful evaluation of histologic material from the uterine cervix will permit the pathologist to exclude those epithelial abnormalities which are not a part of the spectrum of cervical neoplasia and allow him or her to place the patient with cervical neoplasia at the proper stage in the development of the process. With this information the clinician can then intelligently plan appropriate therapy.

Carcinoma in Situ↗
PubMed1975

Response of rat testis to localized induced hyperthermia.

A method is presented in which the rat testis is extensively mobilized through a low abdominal incision, but in which its blood supply is carefully preserved. Localized hyperthermia is induced in this mobilized testis by water bath immersion. The tissue temperature is measured during and after immersion by means of a thermocouple inserted into the tissue. The relative sensitivity of spermatogenic tissue to increased temperature is confirmed and the relative resistance of Sertoli and Leydig cells is noted. Minimal or absent inflammatory reaction to thermal destruction of testicular cells is found as long as the tubule is intact. A marked peritesticular inflammatory response is noted when the total testicular tissue is destroyed at the highest temperature tested.

Animals↗
PubMedDate not supplied

Sampling, sampling errors and specimen preparation.

To obtain an adequate cervical smear for making a correct cytologic diagnosis, smear taking, laboratory handling and interpretation must be optimal. Many people are involved, and only by a combined effort of all links can this target be seriously approached: the smear takers will have to be open minded about technical improvements and read the morphologic descriptions cautiously; in the laboratory, cytotechnicians and physicians will have to challenge themselves and each other. It is mandatory to discard specimens that do not meet general standards of adequacy. At present a host of new techniques are being implemented. It is not feasible for all laboratories to be engaged in testing these new methods, but we are all requested to follow the development the best we can and switch to new ways when justified. Our working conditions are very different; therefore, it is our professional responsibility and plight to respond at the right time. So far the conclusion is that the conventional Pap smear is the international standard of care for the diagnosis of cervical cancer precursers in cancer screening programs. Certainly, this may change within a very short time. Liquid-based techniques, and in particular HPV technologies, are just around the corner.

Cell Biology↗
PubMedDate not supplied

Sensitivity of the AutoPap 300 QC System for cervical cytologic abnormalities. Biopsy data confirmation.

OBJECTIVE: To assess the AutoPap 300 QC System's sensitivity to cases of high grade squamous intraepithelial cervical lesion and invasive cervical cancer, a comparison of outcome was made in cases that had surgical biopsy confirmation. STUDY DESIGN: The sensitivity of the device to biopsy-confirmed diagnostic categories at the level of high grade squamous intraepithelial lesion (HSIL) and greater was calculated. At two of six clinical trial sites, biopsy data were available and were compared to device scores indicating whether a case would or would not be selected for 10% or 20% quality control rescreening. RESULTS: In 86 biopsy-positive cases that had a cytologic diagnosis of HSIL or greater, the device indicated inclusion in the 10% or 20% review fraction for 66 slides (77%) and 74 (86%), respectively. This figure indicated an approximately eightfold improvement in the ability of the device to include such cases for quality control rescreening when compared to a 10% random case selection process. CONCLUSION: These data showed that the sensitivity of the AutoPap in identifying cases of HSIL and greater was similar to that previously calculated for these diagnostic categories in clinical trials, when calculated on the basis of an alternate or "higher" standard of tissue confirmation.

Biopsy↗
PubMedDate not supplied

Fibroxanthosarcoma of the uterine cervix: cytopathologic and histopathologic manifestations.

A case of fibroxanthosarcoma of the uterine cervix is reported with its cytopathologic manifestations. The cellular features of two cell populations characterized by atypical cells of fibroblastic and histiocytic types, suggests the correct diagnosis. The possibility of uterine sarcoma must be considered in the differential diagnosis of bizarre and unusual cytologic findings.

Adult↗
PubMedDate not supplied

Needle cytology of the breast. A quantitative and qualitative study of the cells of benign and malignant ductal neoplasia.

Twenty-seven ductal breast lesions, including cases of intraductal hyperplasia, intraductal carcinoma and infiltrating carcinoma, were studied cytologically by means of the Nordenstrom screw technique. Qualitative and quantitative cellular features were evaluated and correlated with histologic findings in sections taken from the region of needle tracts. This analysis indicated that a marked variation in nuclear size, the presence of macronucleoli, chromatin clumping, marked nuclear overlap and cellular disarray were indicators of malignancy. The last two features were the most specific and sensitive indicators of malignancy whereas the others were specific but only mildly sensitive. Some prediction of the degree of hyperplasia was possible with these morphologic criteria. Infiltrating carcinoma could not be distinguished cytologically from intraductal carcinoma.

Biopsy, Needle↗
PubMedDate not supplied

Plastic-embedded semi-thin sections of fine needle aspiration biopsies with dibasic staining. Diagnostic and didactic applications.

The diagnostic and didactic utility of plastic-embedded semi-thin sections of fine needle aspiration biopsies is presented using a case-study approach. The Spurr epoxy semi-thin sections were stained with a newly developed sequential basic fuchsin-methylene blue stain, which gives hematoxylin-and-eosin-like staining and simultaneously substitutes for a wide variety of special stains. The informational content of the sections can approach that of electron microscopy. The use of a direct off-the-slide "pop-off" technique in preparing the plastic-embedded sections allows for a direct comparison between similar groups of cells embedded in plastic and present on the routine aspiration slides; retrospective analysis can discern subtle, previously unrecognized morphologic features in the alcohol-fixed, Papanicolaou-stained slides. The limitations of this comparative approach, however, become manifest when the effects of alcohol fixation on cells are directly compared in plastic and at the ultrastructural level to aldehyde fixation.

Adenoma↗
PubMedDate not supplied

Epithelial repair and regeneration in the uterine cervix. I. An analysis of the cells.

Cell studies from 1,376 patients with abnormal cells derived from so-called tissue repair or regeneration comprised the basis for this study. Planimetric measurements were performed on camera lucida tractings of 1,573 atypical reparative cells (ARC). A total of 3,377 ARC were anlyzed with respect to cytoplasmic and nuclear features. On the basis of these analyses, ARC were subclassified into three types. In most instances, the morphologic type corresponded with the cervical epithelium of origin. The differential morphologic features of ARC were compared with the morphologic features of endocervidal adenocarcinoma and large cell nonkeratininzing squamous cell carcinoma. The biologic potential and clinical significance of ARC is to be the subject of a subsequent report.

Adenocarcinoma↗
PubMedDate not supplied

Quality assurance in cytopathology. Recommendations and ongoing quality assurance activities of the American Society of Clinical Pathologists.

The recommendations and activities of the American Society of Clinical Pathologists (ASCP) pertaining to quality assurance in cytopathology are reviewed. To assure the early detection of cervical cancer, the ASCP recommends that all women who are, or have been, sexually active should have an annual Papanicolaou smear examination, the reasons for which are discussed. To assure the optimal quality in the evaluation of these smears, the ASCP recommends that the screening should be performed (1) in an accredited laboratory by certified cytotechnologists, (2) for whom realistic workloads have been established, (3) under the supervision of a pathologist adequately trained in cytology, (4) who will maintain an ongoing quality assurance program. In addition, (5) Papanicolaou smear evaluations should be available to all patients at a reasonable cost and (6) the financial incentives for the performance of low-quality screening should be eliminated. The society's quality assurance activities discussed include the Check Sample program, self-assessment examinations, meetings and publications; planned additions to this arena include a challenge exam and proficiency survey mechanisms.

Cytodiagnosis↗
PubMedDate not supplied

Fine needle aspiration cytopathology of retroperitoneal lymph nodes in the evaluation of metastatic disease.

Fine needle aspiration biopsy of retroperitoneal lymph nodes was performed in 47 patients with abnormal lymphograms during the course of evaluation for metastatic disease. The biopsies were positive for malignant tumor in 11 patients. Utilizing established cytopathologic criteria a definitive diagnosis could be established. Fine needle aspiration of abnormal lymph nodes detected by lymphography is an easily performed, accurate diagnostic technique.

Biopsy, Needle↗
PubMedDate not supplied

Pemphigus vulgaris and microinvasive squamous-cell carcinoma of the uterine cervix.

Although patients with disseminated pemphigus vulgaris may have involvement of the uterine cervix, such involvement is often detected only after vaginal discharge or bleeding. When a cervical smear is obtained, distinctive cytologic abnormalities may be observed; these may be attributed to the changes of pemphigus or to an associated reparative/inflammatory reaction. This study documents the first two cases of microinvasive squamous-cell carcinoma of the uterine cervix developing in association with uterine cervical pemphigus. The gross pathologic, cytologic and histologic features of these lesions are illustrated. The cytologic criteria that may be helpful in distinguishing between cells derived from microinvasive squamous-cell carcinoma and pemphigus of the uterine cervix are described.

Adult↗
PubMedDate not supplied

Fine needle aspiration biopsy in the evaluation of lymphoreticular tumors of the thorax.

The role of fine needle aspiration (FNA) biopsy in the evaluation of lymphoreticular disorders involving the lung and mediastinum was evaluated in a study of 16 cases from the authors' institutions. The cytologic material from these cases was reviewed, and the original cytologic interpretations were compared to the tissue diagnoses, with the overall accuracy of the cytologic interpretation evaluated. There were no false-positive diagnoses of malignancy in this series. A definite diagnosis of malignant lymphoma was most readily made in those cases in which the lymphoma was of the large-cell type. Small-cell and mixed large-cell and small-cell lymphoid proliferations were less able to be definitively interpreted, with pseudolymphoma presenting particular difficulty. The results support the conclusion that FNA biopsy can be useful in evaluating these lesions but also emphasize the fact that accurate diagnosis requires correlation of cytologic, clinical and laboratory data.

Aged↗
PubMedDate not supplied

Pulmonary fine needle aspiration cytopathology. A five-year correlation study.

Transthoracic fine needle aspiration specimens with abnormal cytology were obtained from 272 patients between 1976 and 1980 at the University of Rochester Medical Center. A comparison was made between the original specific cytologic and final histologic diagnoses on 116 patients; an additional 16 patients with the cytologic diagnosis of small-cell carcinoma were evaluated by clinical criteria. Analysis of the data indicated that malignant neoplasms were identified correctly with an accuracy of 99%. There was a single false-positive diagnosis. Predictive values for a specific morphologic variant of pulmonary neoplasm were 70% for squamous-cell carcinoma, 86% for adenocarcinoma and 95% for small-cell carcinoma. The probable bases for diagnostic error are discussed. Confidence intervals calculated from these data compared favorably with those in recently reported studies. The results reconfirmed the value of fine needle aspiration cytopathology for the diagnosis of pulmonary neoplasms.

Adenocarcinoma↗