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Biomedical subjects

T A Petrova

Publications and source records attributed to T A Petrova.

At least 19 recordsLinked to original sources
PubMed2002

[The study of partial tension of blood oxygen and forced expiration in patients with chronic obstructive bronchitis receiving inhalation of hypertonic salt solution].

To ascertain side effects of aerosol therapy which implies use of concentrated hypertensive salt solution (HSS), patients with chronic obstructive bronchitis (COB) and marked obstructive ventilation disturbances on HSS inhalation treatment were examined for PaO2 and bronchial patency. It was established that HSS inhalations do not affect PaO2 in COB patients. This expands opportunities for design of aerosol technology and its practical applications.

Administration, Inhalation↗
PubMed2000

[Antibacterial drug resistance of gram-negative agents causing urinary infections in female outpatients in Russia: results of multicenter study].

AIM: To investigate the spectrum of gram-negative agents causing acute and recurrent cystitis in outpatients and sensitivity of uropathogenic E. coli to antibacterial drugs; to compare drug resistance of uropathogenic E. coli isolated in Russia and other countries. MATERIAL AND METHODS: The spectrum of gram-negative bacteria was identified in 299 cases of acute and recurrent cystitis in Moscow, Smolensk and Novosibirsk. 271 E. coli uropathogenic strains were examined according to CA-SFM and NCCLS criteria for sensitivity to ampicilline, gentamycin, trimetoprim, co-trimoxasol, nitrofurantoine, nalidixic acid, pipemidine acid, norfloxacine, ciprofloxacine, nitroxoline. RESULTS: E. coli, K. pneumoniae, K. oxytoca, P. mirabilis, P. vulgaris caused acute and recurrent cystitis in 90.6, 6.4, 1, 1.7, 0.3% of the examinees, respectively. For Moscow relative agents were: E. coli (80.8%), K. pneumoniae (13.1%), K. oxytoca (2.3%), P. mirabilis (3.1%), P. vulgaris (0.7%). In Smolensk E. coli, K. pneumoniae, P. mirabilis were isolated in 96.3, 2.5 and 1.2%, respectively. E. coli occurred in 100% of Novosibirsk cases. Mean Russian values of the resistance to ampicilline, gentamycin, trimetoprim, co-trimoxasol, nitrofurantoin, nalidixic acid, pipemidine acid, norfloxacine, ciprofloxacine, nitroxoline were the following: 33.3, 5.9, 20.3, 18.4, 2.9, 5.5, 4.4, 2.6, 2.6 and 94.1%, respectively. Resistance to 2 and more drugs was registered in 18.4% of E. coli strains. CONCLUSION: Cystitis in women was in most cases caused by E. coli. The highest resistance among uropathogenic strains E. coli was observed to nitroxoline, ampicilline, trimetoprim and co-trimoxasole; maximal antibacterial activity against uropathogenic E. coli was shown by fluoroquinolones (norfloxacin and ciprofloxacin).

Anti-Bacterial Agents↗
PubMed1992

[Changes in creatine kinase activity and protein spectrum of the developing muscle culture treated with creatine phosphate].

A study was made of the influence of creatine phosphate (CP) on creatine kinase activity and the total protein status of the primary chicken myoblast culture. It has been established that the two-hour exposure of the developing culture in the presence of 2 and 4 mM CP is accompanied by the lowering of the content of water extractable protein and a considerable increase of creatine kinase activity. Meanwhile the total activity of the enzyme rises in the non-differentiated and falls in the differentiated one. Electrophoretic analysis has shown CP to produce not only quantitative but also deep qualitative changes in the myoblast protein spectrum. It is assumed that the changes revealed are nonspecific.

Animals↗
PubMed1988

[Biological activity of thymus preparations from sheep of various ages].

Thymus active fractions from 3-year-old sheep were shown to cause immune response stimulation to sheep red blood cells in experimental immune deficiency in mice. The fraction from fetal lamb and sheep thymus was more active than that of mature animals in increasing immune rosette-formation in the lymph of central and peripheral organs of the immune deficient mice. All the preparations recovered the number of splenocytes in immune deficient mice. Therefore, thymus active fractions obtained from sheep at different stages of ontogenesis had a stimulating effect on cell proliferation and immunogenesis.

Aging↗
PubMed1977

A mathematical model of microbial growth including an intermediate. I. Growth in batch cultures.

A mathematical model of microbial growth is presented and examined which, in contrast to the well-known MONOD model, includes transitions from one cell "bottle-neck" to another. This is achieved by introducing an intermediate product in the model. Three variants of the model for different regulatory functions of the intermediate are considered. The results permit to describe a set of experimentally observable microbial growth curves. According to the model, the shape of the growth curves, the kinetics of substrate consumption and changes of intermediate concentration depend on culture prehistory and the nature of the intermediate regulatory function.

Bacteria↗
PubMed1975

[Changes in the functional activity of macrophages, their morphology and metabolism under the influence of several biologically active substances].

The functional activites of peritoneal macrophages, their morphology and metabolism under the effects of some metabolic inhibitors and various biological ngents were studied. A correlation between functions of macrophages (speading on glass, pinoctosis of virus particles), changes in their metabolism (RNA and protein synthesis; specific activity ofcreatinkinase and catepsin), properties of the macrophages plasma membrane, and the number of lysosomes, was found.

Ascorbic Acid↗
PubMedDate not supplied

[beta-Glucosidase of leaves and roots of the common beet Beta vulgaris].

beta-Glucosidases detected in the leaves and roots of common beet, Beta vulgaris, have been demonstrated to catalyze hydrolysis of native betacyanins. A method is described for the isolation and purification of beta-glucosidase from the roots, which involves ammonium sulfate precipitation, DEAE-cellulose chromatography, and Sephadex gel filtration. Maximum activity of the enzyme is detected at 50 degrees C and pH 8.0; it retains stability within the pH range from 5.1 to 9.2. In the leaves, beta-glucosidase is chloroplast membrane-associated; solubilization of the membranes results in the enzyme inactivation.

Chenopodiaceae↗
PubMedDate not supplied

[Comparative enzymologic analysis of the creatine kinases from the skeletal muscles of the cod, frog and rabbit].

Electrophoretic studies have been made of the protein composition and isozymic pattern of the creatine kinase from muscles of the cod. It was shown that this enzyme constitutes up to 40% of total water-soluble proteins of muscle tissue. Isolation and purification procedures were suggested for the creatine kinase from cod muscles which allow to obtain the enzyme with the specific activity 250-350 IU/mg. Comparative enzymic analysis of creatine kinases from muscle tissue of the cod, lake frog, and rabbit was also made. Studies were carried out on temperature dependence of the reaction, kinetic constants at temperatures 5 and 30 degrees C were determined together with other physicochemical properties of the enzymes. The revealed species specific differences are discussed in relation to the structure and function of the enzymes in lower vertebrates in vivo.

Animals↗