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Biomedical subjects

T A Posevaia

Publications and source records attributed to T A Posevaia.

At least 19 recordsLinked to original sources
PubMed1990

[The fluorescent antibody method in the diagnosis of hepatitis B virus infection in glomerulonephritis patients].

In 107 patients with glomerulonephritis (GN) (28 were with acute and 79 with chronic GN) and 54 patients afflicted with other diseases of the kidneys, peripheral blood leukocytes were examined for HBsAg by indirect immunofluorescence. In 60 patients with GN and 32 patients with other diseases, blood sera were examined for HBsAg and anti-HBs by enzyme immunoassay along with examination of blood leukocytes for HBsAg. In blood leukocytes of GN patients, HBsAg was detected in 23.4% of cases, whereas in patients afflicted with other diseases of the kidneys, in 5.6% of cases. In the blood serum, HBsAg was also demonstrated more frequently in GN patients (in 15% of cases) as compared to patients with other diseases of the kidneys (in 3.1% of cases). The rate of anti-HBs demonstration did not significantly differ in patients with GN and in those suffering from other diseases of the kidneys. None of the examined patients both with GN and other diseases of the kidneys showed at a time HBsAg and anti-HBs in the serum or HBsAg in leukocytes and anti-HBs in the blood serum. HBsAg in leukocytes and in the blood serum was identified at a time only in one patient afflicted with chronic GN.

Adolescent↗
PubMedDate not supplied

[Detection of components of type D oncornavirus in the mitochondria of HEp-2 cells].

The HEp-2 cell mitochondria were found to harbor electronoptically dense subvirus particles with a byouant density of 1.27-1.28 g/cm3 which formed in the mitochondria de novo (experiments on incubation of isolated mitochondria with 32P). The intramitochondrial localization of proteins of oncornavirus type D was proven by different methods of the antigenic analysis (CFT, immune precipitation, autoradiography). The additional evidence of the participation of mitochondria in biosynthesis of this virus was obtained by the use of inhibitory tests (a different effect of chloramphenicol and cycloheximide). The possible role of mitochondria in biosynthesis of oncornaviruses is discussed.

Antigens, Viral↗
PubMedDate not supplied

[Oncornavirus D--an inducer of a new antigen].

Oncornavirus D inoculated into chick fibroblast cultures induced in them synthesis of a new antigen which is not incorporated into the virion structure and is absent in cells not infected with this virus. The oncornavirus D newly induced antigen is identical to that found in long-term continuous human HeLa, HEp-2 and J-96 cells. These facts give grounds to believe that the new antigen present in these cells emerged as a consequence of their infection with oncornavirus D. However it remains unknown whether this antigen plays the role of the transforming agent and whether transformation of normal human cells into malignant cells is associated with it.

Animals↗
PubMedDate not supplied

[Oncornavirus D associated antigen in cultured human and animal cells].

A large number of long-term continuous (LTC) and primarily trypsinized (PT) human and animal cell cultures were examined for the presence of a new oncornavirus D-associated antigen. All the PT cultures were found to be free from this antigen as also human diploid cells and LTC cells of man, rabbit, mouse, dog, swine and bat. LTC cells of monkey, bull, and Syrian hamster, however, were found to contain oncornavirus D-associated antigen identical to that previously found in HeLa, HEp-2 and J-96 cells. The detection of oncornavirus D-associated antigen in cells may indicate their infection with this virus.

Animals↗
PubMedDate not supplied

[The laboratory diagnosis of cytomegalovirus infection in infants in the first months of life].

The detection rate of IgM, specific to cytomegalovirus (CMV), in the umbilical blood was 2.0 +/- 1.8% in infants with antenatal complications in their medical history, 8.9 +/- 1.8% in hospitalized infants aged 7 days to 6 months; simultaneously, such IgM proved to be absent in healthy infants of the same age. The maximum primary detection rate of CMV-specific IgM in hospitalized infants was registered at the age of 1.5-4 months and the minimum detection rate, at the age of 6 months, as well as at the age of 1 month and younger. In sick infants aged 0-6 months CMV-specific IgM were detected, as a rule, for 2-4 weeks. 86% of infants with CMV-specific IgM detected in their blood were found to have cytomegaloviremia; in 80% of patients the virus was excreted with saliva and in 50% the virus was detected in blood plasma.

Antibodies, Viral↗
PubMedDate not supplied

[Neutralization of Venezuelan equine encephalomyelitis virus by anticellular serums].

The results of the study of host antigens in Venezuelan equine encephalomyelitis virus by means of neutralization test in tissue culture using sera containing antibody for the species-specific antigens, Forssman antigen and group A and B antigens are presented. They show the inhibiting effect of the sera to be not uniform. The greatest neutralizing effect on the virus was exerted by immune sera to chick embryo fibroblasts and their fractions (by 0.5-3.9 lg PFU/ML). The lack of the virus neutralizing effect in sera for the Forssman antigen and for group A antigen appears to depend upon their deeper localization in the structure of virion.

Animals↗
PubMedDate not supplied

[Properties of new antigens and viral antigens of transplantable cultures of human malignant cells].

Physico-chemical and immunological differences between a new virus-associated antigen of human cell cultures and the antigen of oncornavirus D persisting in them were established and shown to facilitate their detection and differentiation. The activity of the new antigen is lost or decreases considerably after treatment with ether or chloroform but is retained after heating at 60 degrees C. The viral antigen is relatively stable to ether and chloroform but is inactivated at a temperature of 60 degrees C. The peculiar physicochemical properties of the new and virus antigens may be used for search of approaches for preparation of monospecific virus antisera containing no antibody for the new antigen of cells in human tissue cultures.

Antigens, Viral↗
PubMedDate not supplied

[Preparation of monospecific sera against oncornaviruses and oncornavirus-associated antigens of human cultured cells].

A method for preparation of monospecific sera against oncornaviruses and oncornavirus-associated antigens of continouus human cells has been developed on the basis of the results of comparative studies of the properties of viral and virus-associated antigens of the cell. Utilization of an adsorbent from cells free of virus antigen for adsorption of immune sera against oncornaviruses with polyspecific properties resulted in preparation of specific antiviral serum free of antibody to virus-associated antigen of the cell. Sera against virus-associated antigens of the cell containing no antibody to virus were prepared by adsorption with concentrated purified virus isolated from the cell culture.

Antigens, Viral↗
PubMedDate not supplied

[Human hybridoma neutralizing antibodies to the herpes simplex virus].

Human hybridoma cells producing monoclonal antibodies to human herpes simplex virus were generated. The antibody produced by the hybrid cells interacted with herpes virus types I and II in enzyme immunoassay and complement fixation test, had neutralizing activity both in vivo and in vitro. These human monoclonal antibodies may be used for immunotherapy of herpes virus infection.

Antibodies, Monoclonal↗
PubMedDate not supplied

[Herpesvirus infection in patients with chronic glomerulonephritis].

Virological examinations of blood, urine and saliva in 75 patients with chronic glomerulonephritis (CG) revealed, in 95% of them, herpes-virus infections caused by herpes simplex virus, type 1 (34.4%), herpes simplex virus, type 2 (2.6%) and cytomegalovirus (12%) or mixed infections (46%). The infection rate in the control group of children without renal pathology was reliably lower. A majority of CG patients (94%) had a diagnostically significant level of antiherpetic antibodies, class IgG, which also evidence to chronic herpes-virus infection.

Animals↗
PubMedDate not supplied

[Immunity to tumors induced by herpes simplex virus in hamsters].

Preparations of early herpes simplex virus antigen (VIA) and specific antibodies to it were used to induce active and passive immunity to herpes virus-induced tumors in experimental animals. The results demonstrated the increased resistance of animals to transplantation of tumor cells due to vaccination with VIA-containing preparations. Thus, in VIA-vaccinated animals there was a delay in the development of tumors and the survival time was significantly higher than in the controls. The sera of these animals contained antibodies to VIA in high titres. Experiments with passive immunization also gave encouraging results.

Animals↗
PubMedDate not supplied

[Anti-idiotypic antibodies against antibodies to the herpes simplex virus and its early antigens].

Polyclonal anti-idiotypic antibodies (anti-Id-AT or AT2) were produced to AT idiotopes of virion antigens--structural proteins of herpes simplex virus (HSV) and of early virus-induced antigens (VIA). The method of SPRIA established that anti-Id-AT seemed to be associated with AT active centers. Besides, immunization with anti-Id-AT produced anti-anti-Id-AT (AT3) similar in their specificity with antiviral AT or anti-VIA. It was shown that AT2 could be used as a diagnostic preparation instead of specific antigen for the detection of antibody to HSV or VIA in human sera by indirect RIA.

Animals↗