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T Adrian

Publications and source records attributed to T Adrian.

59 records · Page 4Linked to original sources
PubMedDate not supplied

The hexon genes of adenoviruses of subgenus C: comparison of the variable regions.

We report on a 1402-base pair long DNA sequence, encoding for the variable region and parts of the flanking conserved regions of the human adenovirus type 1 (AV1) hexon, and a DNA sequence comprising 1396 base pairs, encoding for the corresponding regions of the human adenovirus type 6 (AV6) hexon. AV1 and AV6, together with AV2 and AV5, belong to subgenus C. Comparison of the predicted amino acid sequences of AV1 and AV6 with those of AV2 and AV5 revealed that the variable hexon region could be subdivided into highly variable, variable and conserved regions. Regions differing between AV2, AV5, AV1 and AV6 were the same as those which were registered as different when comparing AV40 and AV41 (subgenus F), suggesting that these areas of the hexon protein represent type-specific antigenic determinants. Alignment of the amino acid sequences to the known 3-dimensional structure of the AV2 hexon demonstrated that the major amino acid changes were located in the L1 and L2 loops that form the surface of the hexon. Comparison of sequencing data between the serotypes might elucidate questions concerning evolution within subgenus C.

Adenoviruses, Human↗
PubMedDate not supplied

Type- and group-specific polymerase chain reaction for adenovirus detection.

We report on a 1,551-base-pair-long DNA sequence, encoding the variable region and parts of the flanking conserved regions of the human adenovirus type 8 (AV8) hexon, and a sequence comprising 1,404 base pairs, encoding the corresponding regions of the human adenovirus type 31 (AV31). Comparison of the hexon sequences showed that the major sequence changes were located in loops I1 and I2 of the hexon polypeptides which form the surface of the virion. We established a type-specific polymerase chain reaction (PCR), using a combination of a group-specific (general) primer, located in a conserved region of the hexon gene, and type-specific primers located in the region that encodes for loop I2 of the AV8 (subgroup D), AV31 (subgroup A) and AV40 and 41 (both subgroup F) hexon polypeptides. We performed PCR directly from several different clinical specimens or from isolates (AV31). Type-specificity was confirmed by restriction analysis. We also carried out several PCR directly from faecal specimens, using a group-specific primer pair and compared the sensitivity of PCR with that of electron microscopy and enzyme immuno assay.

Adenoviruses, Human↗
PubMedDate not supplied

Recombinant fibre proteins of human adenoviruses Ad9, Ad15 and Ad19: localization of the haemagglutination properties and the type-specific determinant.

The adenovirus fibre carries the type-specific gamma determinant the existence of which was suggested by haemagglutination inhibition tests. Furthermore, the fibre is thought to be responsible for the agglutination of monkey, rat and human erythrocytes. In order to verify that the haemagglutination properties and the type-specific gamma determinant are located on the fibre knob domain, several recombinant fibre proteins of subgenus D adenoviruses were constructed, expressed in HeLa cells and tested in haemagglutination and haemagglutination inhibition tests. Our data showed that the epitopes responsible for the interaction with erythrocytes and the gamma determinant are located on the fibre knob domain.

Adenoviruses, Human↗
PubMedDate not supplied

PCR-based detection and typing of human adenoviruses in clinical samples.

Since human adenoviruses (Ad) are associated with a variety of diseases, there is need for a fast and sensitive diagnostic procedure. The polymerase chain reaction (PCR) has been previously applied for the detection and typing of adenoviruses directly in clinical samples. So far, only Ad8, Ad31, Ad40 and Ad41 could be typed by PCR. To extend the technique of type-specific PCR to other adenovirus serotypes, type-specific primers for Ad1, Ad2, Ad4, Ad5, Ad19 and Ad37 were evaluated. In the present study, 50 stool and 68 eye swab specimens were first tested for the presence of adenoviruses using genus-specific primers. Adenoviruses could be detected in 42 stool and 47 eye swab samples. While the adenovirus-positive stool samples were subsequently typed with primers for Ad2, Ad5, Ad31, Ad40 and Ad41, the positive eye swab specimens were typed with primers for Ad4, Ad8, Ad19 and Ad37.

Adenovirus Infections, Human↗