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Biomedical subjects

T Ahlgren

Publications and source records attributed to T Ahlgren.

30 records · Page 2Linked to original sources
PubMed1985

Plasma fibronectin is initially decreased during septicemia.

Plasma fibronectin (PFN) levels were measured by radioimmunoassay in 24 normals and serially in 24 septic patients without underlying major trauma. All patients responded promptly to antibiotic therapy and none developed signs of shock or disseminated intravascular coagulation (DIC). After an initial decrease in PFN registered in most of the septic patients, the levels were normalized within 2 weeks of antibiotic treatment. The mean nadir levels of PFN were decreased (p less than 0.001) both in patients with gram-negative and gram-positive etiologies compared to the control group. Furthermore, the mean PFN value of the gram-positive group was lower (p less than 0.05) than that of the gram-negative group. It is concluded that a transient depletion of PFN is a constant finding in septic patients with a favourable outcome and that a single low PFN level alone does not justify fibronectin replacement therapy nor does it indicate a poor prognosis.

Adult↗
PubMed1984

Hyaluronic acid enhances phagocytosis of human monocytes in vitro.

Pretreatment of human blood monocytes with hyaluronic acid for 0.5 hr enhanced significantly their ingestion in vitro of yeast particles opsonized with complement. The enhancement of the phagocytic capacity was dose dependent and maximal in the range of 25-50 micrograms/ml.

Cell Adhesion↗
PubMed1983

Loss of surface fibronectin from human lung fibroblasts exposed to cytotoxin from Clostridium difficile.

Clostridium difficile cytotoxin caused an irreversible dose- and time-dependent loss of fibronectin from the surfaces of human lung fibroblasts, paralleling the appearance of the cytopathic effect. Fibronectin was not required for the intoxication process. The results lend further support to a transmembrane connective link between fibronectin and the microfilaments.

Bacterial Proteins↗
PubMed1982

Fibronectin increases the motility, phagocytosis and NBT (nitroblue tetrazolium)-reduction of granulocytes.

Neutrophilic granulocytes preincubated in vitro with human plasma fibronectin (0-1.25 mg/ml) for 30 minutes showed increased spontaneous and chemotactic migration, increased uptake of fluorescein labelled yeast particles as well as higher NBT-reduction both with and without E. coli stimulation. These effects on the granulocyte functions were found to be mainly dose dependent. In control experiments, no significant effects were noted when fibronectin was replaced by human serum albumin in the same concentration range.

Bacterial Infections↗
PubMed1980

Influence of cancer chemotherapy treatment on the reticuloendothelial system in the rat.

The phagocytic function of the reticuloendothelial system (RES) in the rat has been studied after the administration of cyclophosphamide, 5-fluorouracil or a combination of these drugs and vincristine. No impairment in the elimination of 125I-labelled microaggregated human serum albumin was found. A significant reduction in relative and total spleen weight was not paralleled by a decreased splenic uptake of the RES test substance per gram tissue. A significant increase in the relative weight of the lungs was paralleled by a significant increase in the uptake of test substance on a per gram basis. No correr. A general reduction in the cell population of the haematopoietic system has to be assumed. If this reduction is more pronounced in subpopulations of T lymphocytes (suppressor T cells) has to be further tested.

Animals↗
PubMed1980

The effect of immunosuppressive therapy on the phagocytic and metabolic functions of the reticuloendothelial system in renal transplant recipients.

The object of this study was to examine the bearing that immunosuppressive therapy may have on the phagocytic and metabolic functions of the reticuloendothelial system (RES). The phagocytic and metabolic activities of the RES were determined in renal graft recipients before transplantation and at intervals afterwards. The activities were measured as the plasma clearance rate of 125I-labelled microaggregated human serum albumin and the increase in the plasma metabolites of this test substance. The intravascular clearance of microaggregated albumin was significantly depressed at 1-12 days (P less than 0.001), 1.4 months (P less than 0.02) and 6-9 months (P less than 0.01) after transplantation compared to the levels before operation. RES phagocytosis was normal in the 1-3 year group. Furthermore, in all except the 1-4 month group the metabolic activity was significantly reduced. The significant impairment of the RES functions immediately after transplantation was probably due to administration of antilymphocyte globulin and extremely high doses of steroids. After antilymphocyte globulin had been withdrawn the phagocytic capacity of the RES was correlated to the total dose of steroids given over the 12 months prior to the test. The azathioprine regime seems to have had no influence on the RES functions.

Adult↗
PubMed1979

Reticuloendothelial function in human renal allograft recipients.

The phagocytic and metabolic functions of the reticuloendothelial system (RES) were determined, by measuring the plasma clearance rate of 125I-labelled microaggregated human serum albumin and the increase in plasma metabolites of this test substance, in patients with chronic renal failure and in renal transplant recipients at different times after transplantation. All transplant recipients received triple immunosuppressive therapy consisting of azathioprine, corticosteroids, and antilymphocyte globulin. The intravascular clearance of microaggregated albumin was significantly depressed in patients when tested at 1 to 12 days (P less than 0.001), 1 to 4 months (P less than 0.02), and 6 to 9 months (P less than 0.001) after transplantation compared to pretransplantation. The 1- to 3-year transplant survivors had a normal RES phagocytosis. Furthermore, the metabolic RES function in all groups of transplant recipients except the group of patients tested at 1 to 4 months after transplantation was significantly impaired compared to pretransplantation. Administration of antilymphocyte globulin and extremely high daily doses of steroids were probably responsible for the significant depression in the RES functions recorded immediately post-transplantation. The further development of the phagocytic ability of the RES was shown to be correlated to the cumulative dose of steroids given over the last 12 months. The azathioprine regime seemed to have no influence on the RES functions.

Adult↗
PubMed1979

Immunoassay of macrophage stimulating alpha 2 SB (surface binding) glycoprotein in pig plasma following missile trauma.

Pigs were subjected to a standardized missile trauma in the thigh and plasma samples were collected at various times after trauma. The plasma level of an opsonic alpha 2 SB (surface binding) glycoprotein related to macrophage phagocytosis was determined by an agglutination assay using 125I-labelled gelatin particles. The results indicated a transient but significance decrease (p less than 0.01) in the plasma agglutination activity after trauma reflecting a deficiency in plasma alpha 2 SB glycoprotein. One non-surviving animal showed a persistent decrease in the plasma level of alpha 2 SB glycoprotein, while the surviving animals showed a normalization indicating that this agglutination assay may have prognostic value during the course of posttraumatic therapy.

Agglutination Tests↗
PubMed1978

Adsorption of insulin by infusion materials.

Doubt about insulin adsorption has been an obstacle to rational insulin treatment by i.v. infusion. The influence of several factors on adsorption was therefore studied using a radioimmunoassay technique to determine insulin levels. When 8 to 128 IU of insulin were added to 500- and 1,000-ml containers, a linear relationship was found between the amount added and the amount delivered to the patient, permitting the design of a practical nomogram. In other experiments, 32 IU of insulin was added to 1,000 ml containers. The type of insulin did not influence adsorption. Half of the 32 IU added to soda-glass and siliconized bottles were adsorbed, while plastic containers adsorbed less. Neither the type of infusion solution (except for 60% glucose), nor the type of infusion set infuenced adsorption. Human serum albumin prevented adsorption only incompletely.

Adsorption↗