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Biomedical subjects

T Akagi

Publications and source records attributed to T Akagi.

At least 379 records · Page 21Linked to original sources
PubMed1977

Establishment and characteristics of a human pancreatic cancer cell line (HCG-25).

A floating cell line (HCG-25) was established from the metastatic ascitic fluid of a human pancreatic adenocarcinoma of ductal cell origin. The cell line was characterized by the growth in suspension with a doubling time of 15.6 hr, a high cloning efficiency in soft agar and a modal chromosome number of 72. Electron microscopic examination revealed terminal bars in a small number of cells. Production of mucin and immunoglobulins and phagocytosis were not demonstrated. Heterotransplantation of the cells produced tumors, being undifferentiated carcinoma histologically. These characteristics mentioned above confirm that HGC-25 cell line is a human pancreatic cancer cell line.

Adenocarcinoma↗
PubMed1976

Human cell line (HGC-27) derived from the metastatic lymph node of gastric cancer.

A cell line (HGC-27) was established by culture of the metastatic lymph node from a gastric cancer patient diagnosed histologically as undifferentiated carcinoma. HGC-27 cells were polygonal or short spindle-shaped and adhered to glass surfaces as a monolayer. The cells were probably derived from gastric cancer cells, as their origin from mesenchymal tissues can be excluded morphologically and enzyme-histochemically. Enzyme activities were generally negative or low, except for adenosine triphosphatase, lactic dehydrogenase and leucine aminopeptidase. These scanty findings might reflect the undifferentiated character of the original tumor cells. The cloning efficiency was 5.3% in liquid medium and 1.0% in soft agar. The doubling time was about 17 hr. Chromosomal analysis revealed a mode of 109 and 110 chromosomes.

Cell Line↗
PubMed1976

Fetal antigens associated with adenovirus 12 mouse tumors.

The fetal antigen(s) of C3H/BfB/Ki mouse tumor cells induced by adenovirus type 12 was examined by transplantation resistance, local adoptive transfer and in vitro cell-mediated microcytotoxicity tests. Mice immunized with 8 to 9-day gestation fetal cells showed weak resistance to the challenge of small numbers of tumor cells, but immunization with 13 to 14-day gestation fetal cells did not confer transplantation immunity. Local adoptive transfer of multiparous pregnant mouse spleen cells mixed with tumor cells showed a small inhibition of tumor growth in all cases, but primiparous pregnant mouse spleen cells showed a protective effect in only one of three samples. In the cell-mediated microcytotoxicity test, spleen cells taken from the fetal tissue-immunized or multiparous pregnant mice showed a cytotoxic effect to MT-2 cells (adenovirus type 12 tumor cell line) and mouse fetal cells, but not to newborn mouse kidney cells. These results strongly suggest the presence of phase-specific tumor-associated fetal antigen(s) which can evoke transplantation immunity.

Adenoviruses, Human↗
PubMed1976

Establishment and characteristics of a human lung adenocarcinoma cell line.

A permanent cell line (HLC-1) was established from the pleural effusion of a human lung adenocarcinoma. The cell line was characterized by the monolayered and multilayered organoid growth of epithelioid cells with the doubleing time of about 33 hr and the modal chromosome number of 68. Cloning efficiency was 17.9% in liquid medium and 8.3% in soft agar. The cell produced a large amount of epithelial mucin. Electron microscopic examination revealed many secretory granules and terminal bars. They formed spherical aggregates in a gyratory culture which showed adenocarcinoma-like tubular structures histologically. Enzyme-histolochemically, they showed the characteristics of lung adenocarcinoma cells except for a few enzymes such as glucose-6-phosphatase and ATPase. Heterotransplantation of the cells produced the tumor. These characteristics confirm that HLC-1 cell line is a human lung adenocarcinoma cell line.

Adenocarcinoma↗
PubMedDate not supplied

Noninvasive evaluation of systolic pressures of pulmonary artery and right ventricle using contrast-enhanced doppler echocardiography: comparative study using sonicated albumin or glucose solution.

Evaluation of pulmonary arterial pressure is essential for the diagnosis and management of patients with congenital heart disease; it is usually done by cardiac catheterization. An alternative, noninvasive method may be clinically more useful. The purpose of this study was to assess the usefulness of the noninvasive determination of systolic pressure of the pulmonary artery and right ventricle by contrast-enhanced Doppler echocardiography. We selected 30 pediatric patients (28 with trivial or nonsignificant tricuspid regurgitant Doppler signals and 2 with significant tricuspid regurgitant Doppler signals) aged 2 months to 21 years. The flow velocity of tricuspid regurgitation was measured with continuous-wave Doppler of the right ventricular inflow view or left parasternal or apical four-chamber view before and after injection of two types of contrast medium (hand-agitated 5% glucose or sonicated albumin). The systolic pressure of the pulmonary artery was assessed as the estimated right ventricular systolic pressure (albumin method) minus the peak pressure gradient across the pulmonary valve (nonenhanced Doppler method). After injection of hand-agitated 5% glucose and sonicated albumin, trivial tricuspid regurgitation signals were enhanced in 25 of 28 patients (89%). In two patients, spectral envelopes were well defined enough to obtain the peak systolic velocity of the tricuspid regurgitation jet without contrast medium injection. Peak velocity was not altered by injection of contrast medium in these patients. There was significant correlation between the estimation by contrast-enhanced Doppler using hand-agitated 5% glucose and the cardiac catheterization measurement of the transtricuspid pressure gradient (r = 0.88). The transtricuspid pressure gradients obtained by continuous-wave Doppler during sonicated albumin enhancement corresponded closely to those measured by cardiac catheterization (r = 0.95). Pulmonary arterial and right ventricular systolic pressures measured by Doppler using sonicated albumin and those obtained by cardiac catheterization were highly correlated (right ventricle, r = 0.96; pulmonary artery, r = 0.95). In conclusion, this technique may be a valuable noninvasive method for determining accurate right ventricular and pulmonary arterial systolic pressures.

Adolescent↗