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Biomedical subjects

T Alderson

Publications and source records attributed to T Alderson.

At least 37 records · Page 2Linked to original sources
PubMed1983

Detection of lymphocytes in malignant gliomas by monoclonal antibodies.

Lymphocytes are not present in normal brain but are known to infiltrate malignant gliomas. The reasons for this infiltration and the signals involved are not understood. The lymphocyte content of 15 malignant gliomas removed at time of surgery was studied, using monoclonal antibodies to T and B cell surface antigens and immunofluorescence. An average of 41% of infiltrating cells possessed surface Ig, with 21% bearing the common T cell marker. There was no evidence of oligoclonal restriction of light chain types.

Adolescent↗
PubMed1982

Human hybridomas from malignant gliomas.

Intratumoral lymphocytes from 12 patients undergoing craniotomy for malignant glioma were fused with a specially derived human myeloma line LICR-LON-HMy2. 71 stable hybridomas were obtained from 5 of the 12 patients. Such hybridomas had a DNA content equal to the sum of that present in lymphocytes and the parent myeloma. The hybridoma supernatants contained human monoclonal immunoglobulins. Glioma-binding activity was detected in 7 out of the 71 supernatants. These results suggest that malignant gliomas contain a population of B lymphocytes which may be involved in host defence against the tumour.

Adolescent↗
PubMed1980

H 9/25 monoclonal antibody recognizes a new allospecificity of mouse lymphocyte subpopulations: strain and tissue distribution.

C3 H/He-mg mice were immunized with C57BL/10 (B10) spleen cells and the immune spleen cells were fused with BALB/c myeloma cells (NS1). One of the monoclonal antibodies (H9/25 antibody) produced by the hybrid cells was studied. It reacts with subpopulations of B10 lymphocytes as well as some lymphoid tumor lines including some of the Abelson virus-induced leukemias. The antigen recognized by H9/25 antibody is expressed on lymphocytes from all the B10 congeneic mice tested as well as some other strains of mice. No linkage between genes coding for the antigen and H-2 loci was found as judged by its presence on cells of the B10 strains regardless of H-2 type and the distribution of the antigen on Bailey recombinant inbred mice. The antigen is expressed on subpopulations of lymph node cells, spleen cells, thymocytes and bone marrow cells. The strain distribution of the H9/25 antigen seems to be identical to that of Ly-6, Ly-8 and Ala-1 antigens. However, the tissue distribution of the antigen recognized by H9/25 antibody, while similar to these alloantigens, is unique and the antigen may be distinct from the other alloantigens.

Animals↗
PubMed1980

Monoclonal anti-A from a hybrid-myeloma: evaluating as a blood grouping reagent.

A monoclonal anti-A antibody has been evaluated and found suitable for use as a potent routine ABO grouping reagent, without the use of additives. The IgM anti-A (MH2/6D4) is secreted into the tissue culture supernatant by a permanent line of cloned cells derived by fusion of anti-A producing spleen cells and a mouse myeloma cell line. This is a cost-effective reagent which should reduce production costs by over 50%. This reagent has the advantages inherent in monoclonal antibodies among them the availability of unlimited quantities of unvarying antibody of known properties.

ABO Blood-Group System↗
PubMed1977

The induction of mutations to 2-thioxanthine resistance in inhibitor depleted conidia of Aspergillus nidulans by gamma-radiation in the presence of oxygen or nitrogen.

A strain of Aspergillus nidulans has been used to study the inactivating and mutagenic effect of 60Cogamma-rays in the presence of oxygen or nitrogen. Mutation was studied by the 2-thioxanthine system which selectively detects forward mutation at a number of gene loci (at least 16). Mutants resistant to conidial pigmentation effects of 2-thioxanthine can be divided into four main classes (2TxR, hx, uaY and cnx) and three of these classes (hx, uaY and cnx) can be further characterized at the gene level. The results demonstrate the existence of a marked differential forward mutational response of gene loci in Aspergillus conidia which is dependent upon whether gamma-irradiation in aqueous suspension was carried out in the presence of oxygen or of nitrogen: this effect is independent of the dose of radiation exposure. The specificity for mutation is altered by the presence of the self-inhibitor of germination for the anoxic treatment but not the oxic irradiation. The implication of different oer's (oxygen enhancement ratios) for mutation induction in different classes or genes for the mechanism and type of damage induced by the two radiation conditions are discussed.

Aspergillus nidulans↗
PubMed1976

UV mutagenesis in inhibitor depleted conidia of Aspergillus nidulans.

UV treated conidia of a strain of Aspergillus nidulans (meth Gl. biAl) depleted of germination inhibitory substances have been examined for inactivation and mutation induction at groups of suppressor gene loci defining three classes of methionine revertants. An exponential decline in the colony forming ability and quadratic increase in mutation frequency (for each class of revertant) as the incident dose increased were observed. The induced mutation frequency for each class and the loss of colony forming ability of the conidia are greater in the absence than in the presence of these self-inhibitors of germination. However, the relative frequencies of the individual classes of revertants did not differ whether the inhibitory substances were present or not. Liquid holding effects leading to increases in survival and mutation have been observed, but the relative frequencies of the individual revertant classes remain unchanged.

Aspergillus nidulans↗