Biomedical subjects
Publications and source records attributed to T Andres.
The marginal adaptation of six composites and one resin based inlay system was investigated. For all fillings maximum stress increased with time. Three days after insertion maximum stress increased up to more than 450 N for almost all fillings.
Neoplastic cells from 13 cases of hairy cell leukemia were investigated for immunoglobulin production and lysozyme activity by an electron-immunoperoxidase technique. In 10 cases cytoplasmic immunoglobulins were found, but lysozyme activity was absent in all cases. Immunoglobulins were detected in the perinuclear space and endoplasmic reticulum and at the surface of hairy cells. Of the cases in which immunoglobulins were detected in hairy cells, nine were positive with IgM antiserum and one with IgG antiserum. The immunoglobulins were monoclonal in all cases; six were positive with lambda antiserum and three with kappa antiserum. The class and type of surface immunoglobulins were identical to those of cytoplasmic immunoglobulins in the hairy cells. These results support the conclusion that hairy cells are commonly derived from immunoglobulin-producing B cells at an earlier stage of differentiation than plasma cells.
The peroxidase-antiperoxidase (PAP) method was used to demonstrate group B streptococci in formalin-fixed, paraffin-embedded tissue sections using commercial group specific streptococcal antiserum. Sections from neonatal necropsies in which postmortem lung cultures were positive for group B streptococci showed positively stained intra- and extra-cellular cocci in the alveolar exudate. Many hyaline membranes contained intact organisms whereas some membranes showed only diffuse positive staining. One case of neonatal death was evaluated in which there was a clinical diagnosis of group B streptococcal infection, but postmortem cultures were not obtained. Group B streptococci were identified in the lung by the PAP method. This method may be of assistance in the postmortem evaluation of certain infectious diseases such as those due to group B streptococci.
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