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T Aotsuka

Publications and source records attributed to T Aotsuka.

22 records · Page 2Linked to original sources
PubMed1989

[Study on genetic polymorphism of isozyme in natural populations of Drosophila virilis].

Using techniques of polyacrylamide slab electrophoresis and agarose electrophoresis, we have detected genetic variation at 6 loci which coding for enzymes in 4 local samples from natural population of Drosophila virilis. We found 50% of the loci detected are polymorphic, depending on the criterion of polymorphism used. An individual is heterozygotes on the average at 27.13% of its loci. The amount of genetic variation fluctuates widely from locus to locus. At Est-alpha, Est-beta, Amy, most of the individuals are heterozygotes. At the other extreme , Mdh, aGpdh, Acph, few individuals are heterozygotes. For Mdh, we have measured the thermostability at 53 degrees C. No more genetic variation was found. We have measured the amount of genetic differentiation between different local populations. The result showed that there is no relationship between geographical distance and genetic distance. The results are discussed in the light of the continuing controversy over selection and natural theories of genetic variation. We think that both selection and stochastic processes must operate simultaneously in most systems.

Animals↗
PubMed1979

Frequency distribution of histocompatibility-2 antigenic specificities in the Japanese wild mouse genetically remote from the European subspecies.

Fifty-eight Japanese wild mice, Mus musculus molossinus, collected from twenty-eight localities were surveyed for twelve H-2 antigens using the haemagglutination method. Significantly higher frequencies of H-2.3 and H-2.5 specifities and relatively lower frequencies of the other public specificities were observed. This was confirmed by examining four specificities, H-2.3, 5, 13 and 23, in 370 mice. Quantitative absorption of the alloantisera by erythrocytes and spleen lymphocytes of molossinus mouse revealed definite absorption of H-2.3, 5 and 8 antigens, though their antigenic strength was apparently weaker than the controls, B10 congenic mice. Comparative assay of the strength of H-2.5 antigen in either homozygous and heterozygous conditions distinctly eliminated the possibility of gene-dose effect for the reduced strength of the public antigens in molossinus mouse. To explain this, divergence time between molossinus and domesticus was computed based on the allelic frequency data already reported on ten loci in both subspecies. It is roughly 1.5 x 10(6) years, which could allow for the occurrence of considerable genetic changes in many public antigens, except those conserved through selection.

Animals↗
PubMed1977

A simplified method for reading hemagglutinations on a flat-bottom microtitration plate in the mouse H-2 assay.

Kaliss' method for determining hemagglutination titer of mouse alloantiserum was modified to a simpler form. It became possible to read the hemagglutination directly on a flat-bottom microtitration plate using an inverted type microscope. Sensitivity of the modified method was almost the same as that of the original one. This method seems to be useful especially for the larger scale assay of H-2 specificities.

Animals↗