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T Bolin

Publications and source records attributed to T Bolin.

25 records · Page 2Linked to original sources
PubMed1980

Subcellular fractionation of human intestinal mucosa by large-scale zonal centrifugation. I. Characterization of subcellular organelles in the distal part of the ileum.

The subcellular organelles in human ileal mucosa have been characterized by large-scale rate zonal and isopycnic density-gradient centrifugation. Mucosal scrapings were obtained from surgical specimens and the cells disrupted by extrusion under controlled pressure. The homogenized cells were then centrifuged to prepare a cell extract and the extract fractionated in a single-step procedure by zonal centrifugation on a continuous sucrose gradient. The subcellular organelles (brush borders, basal-lateral membranes, lysosomes, mitochondria, and endoplasmic reticulum) were located in the density gradient by assay of marker enzymes. Since a large number of analyses can be done on each fraction of interest, this fractionation technique may be used to study a variety of compounds and concepts involved in the subcellular physiology and pathology of human ileal mucosa.

Adult↗
PubMed1980

Subcellular fractionation of human intestinal mucosa by large-scale zonal centrifugation. Characteristics of lysosomes and brush border membranes in the distal ileum of a patient with Crohn's disease.

Lysosomes and brush border membranes from diseased ileal mucosa of a patient with Crohn's disease were separated by rate zonal density gradient centrifugation. The organelles were located and characterized in the density gradient of assay of marker enzymes, and their enzyme content compared to that of similar organelles from normal tissue. The activity and latency of lysosomal enzymes (N-acetyl-beta-glucosaminidase and acid phosphatase) was significantly increased, whereas the activity of brush border alkaline phosphatase was rather decreased. The possible relevance of these findings to the pathophysiology of Crohn's disease is discussed.

Acetylglucosaminidase↗
PubMed1980

Subcellular fractionation of human intestinal mucosa by large-scale zonal centrifugation. Separation of the organelles in diseased ileum of a patient with Crohn's disease.

The subcellular organelles in diseased ileal mucosa of a patient with Crohn's disease have been separated by rate zonal density gradient centrifugation. Mucosal scrapings were obtained from a surgical specimen of diseased intestine and the cells disrupted by extrusion under controlled pressure. The homogenized cells were then centrifuged to prepare a cell extract and the extract fractionated in a single-step procedure by zonal centrifugation on a continuous sucrose gradient. The subcellular organelles (brush borders, basal-lateral membranes, lysosomes, mitochondria and endoplasmic reticulum) were located in the density gradient by assay of marker enzymes and their overall distribution compared with the distribution of organelles from a normal tissue. The centrifugal properties of individual organelles in the diseased tissue were largely unaffected but the lysosomes were more fragile than in the normal tissue. Since the fractionation technique used is on a large scale, each fraction of interest can be further analyzed for a variety of compounds. Therefore, the subcellular pathology and pathophysiology in this and other cases of Crohn's disease may eventually be studied and discussed in other than morphological and descriptive terms.

Aged↗
PubMedDate not supplied

Digital subtraction angiography versus standard contrast arteriography in evaluation of peripheral vascular disease.

Fifteen patients with lower limb ischemic disease were preoperatively assessed with both intravenous or intra-arterial digital subtraction angiography (DSA), and standard contrast angiography (SCA). The images were reviewed and the operative strategies, based on the clinical data and each of SCA and DSA, were compared. SCA provided more information than DSA in two patients, one of which had an incomplete DSA investigation. DSA provided more information than SCA in three patients. The distal crural vessels were better visualized with DSA in one patient, and popliteal lesions were demonstrated as being more severe using DSA than with SCA in two patients. The level of the distal anastomosis of the proposed bypass was lowered to below the knee as a result of DSA in these two patients. We conclude that comparable information is obtained using SCA or DSA, and that DSA, in particular the intraarterial technique, is a satisfactory alternative to SCA in preoperative evaluation of patients with lower limb ischemic disease.

Aged↗