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T C Bruice

Publications and source records attributed to T C Bruice.

At least 127 records · Page 7Linked to original sources
PubMed1971

On the concept of orbital steering in catalytic reactions.

Angular displacement from linear overlap of but a few degrees in the transition state of the enzyme-substrate complex has been postulated to be of great kinetic significance ("orbital steering"). The concept of orbital steering is shown to have evolved from the orientation parameters of an equation previously proposed to evaluate the kinetic importance of propinquity. This equation is shown to be naive. Arguments provided against the concept of orbital steering include: (a) force constants predicted from orbital steering are about 100 times those experimentally determined from displacement of nuclei in a direction normal to the axis of a covalent bond (for example, at room temperature vibrational bending amplitudes of +5 degrees or more are common); (b) because of the lessened directionality of orbitals containing nonbonded electron pairs, the force constants in transition states should be even smaller than in the case of a covalent bond; and (c) molecular orbital calculations predict shallow total energy minima for orbital alignment. The experimental rate data offered as a basis for the concept of orbital steering are shown to find rationalization in the previously observed dependence of DeltaSdouble dagger on kinetic order and the energy requirements for the freezing-out of single bonds in the transition state leading to the formation of medium-size ring compounds from extended ground states. It is concluded that if orbital steering does exist, experimental and theoretical evidence to support this concept have yet to be presented.

Journal Article↗
PubMed1970

Biotin-bound CO2 and the mechanism of enzymatic carboxylation reactions.

Considerations are provided which show that the carboxylation site (oxygen or nitrogen) of biotin is most likely unknown. Kinetic studies of intramolecular reactions of the ureido function at acyl carbon demonstrate that ureido oxygen attack is the preferred mode when the acyl group is activated. Transfer from the O-acylisourea formed by this route (relative to an N-acylurea) is also favorable. These model studies are related to the carboxylation and CO(2) transfer steps of biotin-mediated reactions, and a mechanism consistent with these results is postulated.

Biotin↗