Simple methods to enrich drug-sensitive lymphocytes in allergic drug hepatitis.
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Biomedical subjects
Publications and source records attributed to T Enomoto.
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Utilizing techniques of Sephadex G-200 column chromatography, sucrose density gradient ultracentrifugation and dextran-coated charcoal adsorption, the characterization of progesterone-binding proteins was attempted in cytosols obtained from the medial basal hypothalamus including the median eminence, the remaining hypothalamus and the cerebral cortex of ovariectomized estrogen-primed rats. Ater incubation of the medial basal hypothalamic cytosol with 3H-progesterone in the absence or in the presence of various unlabeled steroids, a specific progesterone-binding protein was found in the 7 S region with its binding capacity of approximately 2 X 10(14) moles per mg protein and dissociation constant of approximately 6 X 10(-9) M. No specific binding was, however, found in the remaining hypothalamus and the cerebral cortex.
A case is reported in which an aneurysm arising at the junction of the right internal carotid artery and a persistent primitive trigeminal artery ruptured to form a carotid-cavernous fistula. The internal carotid artery was ligated without any signs of brain-stem ischemia due to inverted blood flow through the primitive trigeminal artery.
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The surface topology of the lymphocytes in human tonsils was performed by means of the scanning electron microscope (SEM). It was revealed that human tonsils consist of both 40.7% villous and 26.6% smooth surface lymphocytes. In general, the percentage results agree with those of E and EAC binding lymphocytes of human tonsils, as previously reported. Our serial experiments using immunological techniques proved the existence of two lymphocytes (T and B cell) having immunological roles.