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T F ANDERSON

Publications and source records attributed to T F ANDERSON.

At least 19 recordsLinked to original sources
PubMed1963

STRUCTURE OF TYPE 5 ADENOVIRUS. II. FINE STRUCTURE OF VIRUS SUBUNITIS. MORPHOLOGIC RELATIONSHIP OF STRUCTURAL SUBUNITS TO VIRUS-SPECIFIC SOLUBLE ANTIGENS FROM INFECTED CELLS.

Purified type 5 adenovirus was disrupted at pH 10.5 and the capsid shown to be comprised of two characteristic morphological subunits: (a) Hollow, polygonal structures corresponding to the virus capsomeres seen in preparation of purified virus and (b) thread-like strands also identifiable in preparations of purified virus. These structures were compared morphologically with purified preparations of the group- and type-specific soluble antigens characteristically produced in mammalian cells infected with adenoviruses. The group-specific soluble antigen was a homogeneous preparation of hollow, polygonal rods identical with the virus capsomeres. The type-specific soluble antigen corresponded to the thread- or fiber-like components of the purified virus particle. Inspection of disrupted virus preparations confirmed earlier immunological data which indicated that the major virus antigen was the group-specific soluble antigen. These data provide convincing evidence for the hypothesis that the adenovirus-induced soluble antigens represent virus subunits produced in excess.

Adenoviridae↗
PubMed1952

The intracellular growth of bacteriophages. II. The growth of T3 studied by sonic disintegration and by T6-cyanide lysis of infected cells.

The growth of the virus T3 has been followed by breaking up the complexes it forms with host cells at various stages in their development and then assaying the debris for active virus particles. Two independent methods for breaking up cells were used: sonic vibration and lysis by the T6-cyanide method previously used for the study of the growth of T4. During the first half of the latent period both treatments, as well as cyanide alone, destroyed the capacity of the complexes for producing daughter virus particles. Furthermore, the infecting particles could not be recovered from them during the first half of the latent period. After the complexes had had 12 minutes of incubation at 30 degrees C. both methods freed daughter virus particles from them in numbers which increased steadily with time until, near the end of the rise period, the normal burst size was reached. In general the agreement between the two yields is so good that one may conclude that both methods liberate quantitatively the mature daughter T3 particles which exist in the complexes before normal lysis occurs.

Bacteriophages↗