Biomedical subjects

T Fazio

Publications and source records attributed to T Fazio.

57 records · Page 4Linked to original sources

PubMedDate not supplied

Liquid chromatographic determination of trace residues of polynuclear aromatic hydrocarbons in smoked foods.

A liquid chromatographic (LC) method was developed and applied to the determination of polynuclear aromatic hydrocarbons (PAHs) in a variety of smoked, market basket commodities. The PAHs are extracted with 1,1,2-trichloro-1,2,2-trifluoroethane (Freon 113) from alcoholic KOH digests of the commodities. The extracts are purified by column chromatography through a deactivated silica gel/alumina column and by liquid-liquid partitioning between dimethyl sulfoxide and cyclohexane before separation of the PAHs by LC. Both fluorescence and UV detectors are used to monitor the LC column effluent to detect nanogram quantities of PAHs. Trace levels of carcinogenic and non-carcinogenic PAHs were found in all samples analyzed, although generally at less than 1 ppb levels.

Animals↗

PubMedDate not supplied

Rapid column method for determination of N-nitrosodimethylamine in malt.

A rapid column elution method has been developed for the determination of N-nitrosodimethylamine (NDMA) in malt. The average recovery of NDMA added to malt at the 10 ppb level was 87%. When a single malt sample was analyzed 10 times, the NDMA found averaged 8.1 ppb with a coefficient of variation of 3.93%. In a study of 15 malt samples, data obtained by the column elution method were in good agreement with those from a vacuum distillation method.

Chromatography↗

PubMedDate not supplied

Liquid chromatographic determination of aspartame in dry beverage bases and sweetener tablets with confirmation by thin layer chromatography.

A liquid chromatographic method is described for the determination of aspartame in dry beverage bases and sweetener tablets. The sample was mixed with the mobile phase, the pH was adjusted to within +/- 0.1 pH unit of the mobile phase, and the sample was diluted to volume with the mobile phase. The solution was filtered and a 10 microL aliquot was injected onto a C18 reverse phase column. Aspartame was quantitated with an ultraviolet detector. Recoveries of aspartame ranged from 94 to 111%. The dry beverage bases contained 5-13% aspartame and the sweetener tablets contained 19% aspartame. The presence of aspartame was confirmed by using thin layer chromatography.

Aspartame↗