Genomic organization and regulation of the human thromboxane synthase gene.
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Biomedical subjects
T Fleischer
Publications and source records attributed to T Fleischer.
Differential effect of human interferon on normal and neoplastic T- and B-cell lymphocytes.
The effect of human interferon (HIF) on the growth and function of normal and neoplastic proliferating human T and B cells was studied. Cell cycle analysis by flow-cytometry showed that up to 2000 units of HIF had little effect on the proliferating fraction (that is, the present S + G2 + M phases of the cell cycle) of normal T cells grown continuously wit growth-promoting factor from PHA-stimulated lymphocytes, while 500 units of HIF suppressed the percent S + G2 + M of the acute lymphocytic leukemia (ALL) T-cell line, MOLT, by 36%. Up to 2000 units of HIF had a moderate enhancing effect on the percent S + G2 + M of PWM-stimulated lymphocytes. In striking contrast, a single unit of HIF caused nearly 40% suppression of the percent S + G2 + M of Daudi, a Burkitt's lymphoma cell. One-thousand units of HIF did not decrease the number of antibody-forming normal cells, and up to 500 units of HIF did not inhibit the total Ig secreted by these cells. These results suggest that amounts of HIF affecting the proliferation of some neoplastic lymphoid cells has little effect on the proliferation of normal T and B cells. In addition, HIF does not appear to affect polyclonal induction of B-cell differentiation.
Antibody Formation↗
Characterization of the immunosuppressive state during Schistosoma mansoni infection.
Analysis of a murine model of schistosomiasis revealed that both the thymus (T)- and bursa (B)-derived compartments of the immune system are modified during acute infection. The functional capacity of T and B lymphocytes to respond to mitogenic stimuli and the humoral response to thymus-dependent (SRBC) and thymus-independent (DNP-Ficoll) antigens are severely depressed. In addition, it was found that suppressor cells capable of inhibiting the response of normal lymphocytes to SRBC arise during acute infection. Although the splenic frequency of T (theta) and B (Ig+) cells remained constant during chronic infection, quantitative changes were detected in each population. In the T cell pool there was a decrease in the percentage of Ly-1+ cells and a concomitant increase in Ly-1+, 2+, 3+, cells, whereas the B cell pool showed a progressive loss of complement receptor-bearing lymphocytes, which apparently was the result of inactivation of surface complement receptor by a serum factor specifically found in infected mice. Characterization of the serum factor strongly suggests it is an immune complex. Thus, it appears that both suppressor cells and immune complexes contribute to changes noted in the immune system during acute schistosomiasis. Additional studies carried out in mice after unisexual infection revealed that egg production is not a necessary prerequisite for several of the immunologic phenomena associated with acute schistosomiasis.
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