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Biomedical subjects

T Fujieda

Publications and source records attributed to T Fujieda.

At least 19 recordsLinked to original sources
PubMed1995

Ruminally protected lysine and methionine for lactating dairy cows fed a diet designed to meet requirements for microbial and postruminal protein.

Dairy cows, 20 at each of two sites, were used to determine responses to ruminally protected Lys and Met in a full lactation study. Cows were fed corn silage twice daily for ad libitum intake and a concentrate four times daily in proportion to milk production. At Truro, cows were fed 2.7 kg/d of alfalfa and timothy hay DM at 0600 and at 1500 h. At Fredericton, cows were fed 2.7 kg of timothy silage DM at 0600 h and 2.7 kg of alfalfa hay DM at 1500 h. Diets were designed to meet, but not to exceed, recommendations for ruminally degradable CP and intestinally digestible protein. Ten cows at each site were fed ruminally protected L-Lys.HCl (19 g/d) and DL-Met (6.5 g/d). Cows fed AA at each site produced more milk, lactose, protein, and fat; milk protein and fat percentages were also higher. No time x treatment interactions occurred for any production parameter. In spite of similar production responses between sites, cows fed AA consumed more DM at Truro, but those at Fredericton did not. Thus, gross efficiency of utilization of dietary N for milk N was increased with AA at Fredericton but not at Truro. However, considering the increased intake of CP by cows fed AA at Truro, an event that would have been expected to depress efficiency of utilization of dietary N, the lack of difference at Truro between treatments can be interpreted as an improvement, relative to expectations, because of AA feeding. High producing dairy cows fed a diet that was adequate in CP responded to ruminally protected Lys and Met primarily with increased production of milk protein and fat throughout the full lactation.

Animals↗
PubMed1986

Polymyositis associated with dissecting aneurysm of arteries and intracerebral hemorrhage.

Dissecting aneurysm and stroke are a rare complication of polymyositis. The present report describes an autopsy case of a 53-year-old woman, who suffered from polymyositis accompanied by dissecting aneurysms of right external iliac and right renal arteries, and furthermore, intracerebral hemorrhage. The latter was caused by necrotizing angiitis. The patient had no abnormal anatomical changes such as coarctation of aorta, aortic stenosis, bicuspid aortic valve or Marfan's syndrome. Atherosclerosis in the patient was mild, and cystic medial necrosis of aorta and arteries was not found. Since the patient was attacked by dissections of arteries following long-term steroid therapy, a possibility can be raised that arterial dissection was attributable to steroid treatment besides necrotizing angiitis complicating in polymyositis.

Aortic Dissection↗
PubMed1985

Sulfate esters of hydroxymethyl-methyl-benz[a]anthracenes as active metabolites of 7,12-dimethylbenz[a]anthracene.

7-Hydroxymethyl-12-methylbenz[a]anthracene (7-HMBA) and 12-hydroxymethyl-7-methylbenz[a]anthracene (12-HMBA), carcinogenic major metabolites of 7,12-dimethylbenz[a]anthracene (DMBA) in untreated rat liver, showed high mutagenic activities toward Salmonella typhimurium TA 98 after preincubation with a sulfotransferase-PAPS system consisting of ATP, sodium sulfate, and a post-mitochondrial fraction (S-9) or a soluble supernatant fraction (S-105) from untreated rat liver. The 7- and 12-HMBAs themselves induced His+ mutation in TA 98 only slightly after preincubation with S-9 in the presence of an NADPH-generating system. Mutagenicity of DMBA toward TA 98 after preincubation with S-9 in the presence of the NADPH-generating system was remarkably enhanced by the addition of ATP and sodium sulfate. The active metabolites, 7-HMBA sulfate and 12-HMBA sulfate, were isolated from these preincubation systems and identified by comparison with the corresponding synthetic specimens. The sulfuric acid ester conjugates were potent mutagens toward TA 98 in the absence of rat liver subcellular fractions. The conjugates bound covalently at significant rates to calf-thymus DNA as well as to S-105 proteins at 37 degrees and pH 7.4 through the 7- or 12-methylene carbon with concomitant loss of their sulfate group. In the presence of S-105, glutathione inhibited the mutagenicity of the metabolically formed or exogenously added 7- and 12-HMBA sulfates. The non-mutagenic glutathione conjugates were isolated from the incubation mixtures and identified as S-(12-methylbenz[a]anthracen-7-yl)methylglutathione from 7-HMBA or its sulfate and S-(7-methylbenz[a]anthracen-12-yl)methylglutathione from 12-HMBA or its sulfate.

9,10-Dimethyl-1,2-benzanthracene↗
PubMed1977

Electron microscopic studies on the cerebral lesions of rats in experimental chronic disulfiram poisoning.

Following chronic administration of disulfiram to rats, changes of the brain were examined electron-microscopically. Pathological findings were observed in the nerve cells of the cerebral cortex and hypothalamus at later stage and synaptic changes in the hypothalamus from initial stage. On the other hand, changes of myelinated fibers, neuroglias and capillaries were very slight. It was considered that neurons were affected more predominantly than other neuronal elements by the cytotoxic action of the drug, and that the synaptic changes of the hypothalamus might reveal chronic disturbance of noradrenergic transmission by inhibition of dopamine-beta-hydroxylase. These ultrastructural findings might relate to the pathogenic mechanism of the disulfiram psychosis.

Animals↗
PubMed1975

An application of neuroendocrinological studies in autistic children and Heller's syndrome.

The response of plasma 11-hydroxycorticosteroids (11-OHCS) to intravenous pyrogen as well as the circadian rhythm of plasma 11-OHCS levels were investigated in seven autistic children and in two children with Heller's syndrome. In autistic children, the stress response, which is acquired in an earlier stage of development, was adequately sustained. However, the circadian rhythm, which seems to appear at a later stage with the maturity of the CNS, frequently revealed abnormal patterns. Similar findings were obtained in the Heller's syndrome cases, indicating organic changes in the brain. On the basis of these results, it is postulated that in early infantile autism there exist some functional changes in the CNS that show a close correlation to the regulatory mechanism of ACTH secretion.

11-Hydroxycorticosteroids↗