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T Fujioka

Publications and source records attributed to T Fujioka.

At least 361 records · Page 20Linked to original sources
PubMed1986

Investigation of blood group antigens and carcinoembryonic antigen in urinary bladder carcinoma.

Blood group antigens and carcinoembryonic antigen (CEA) were sought in the tissues of 37 bladder tumor cases using enzyme antibody method. A correlation (p less than 0.01) was found between the extinction of ABO (H) and progressive malignant states of the tumor or invasion stage. Significant correlation (p less than 0.05) was observed between the presence of CEA and the invasion stage. The state of the T antigen, however, did not reflect the malignancy grade or invasion stage. The states of ABO (H) and CEA significantly reflected (p less than 0.05) the prognosis, as did the malignancy grade and invasion stage, while the state of the T antigen did not correlate with the prognosis. Although there was a correlation (p less than 0.01) between the extinction of ABO (H) blood group antigens and the presence of CEA, the state of T antigen did not significantly correlate with ABO (H) antigen or CEA. From what has been mentioned, the search for intratissue ABO (H) antigens and CEA is important and will provide a useful auxiliary means in diagnosing biopsy specimens.

ABO Blood-Group System↗
PubMed1986

[A study of the antineoplastic activity of K18].

K18 is a new drug produced by a combination of Melphalan and human immunoglobulin. K18 produced an inhibitory effect on the proliferation of human gastric and colon cancer transplanted in to nude mice. Also, combination effects of K18 with MMC and 5-FU were recognized in the same system. In a study of distribution after K18 administration, the accumulation and retention of this drug in the tumor region were observed. In the case of Melphalan administration, these phenomena were not observed. The antitumor activity of tumor homogenate was evaluated using a colony-forming assay with KB cells. As to the Melphalan-treated group, a four-hour homogenate reduced the number of colonies but a 48-h one did not. In the K18-treated group, the four-hour homogenate decreased the number slightly and the decrease became obvious with 48-h homogenate. In cell cycle analysis using K18 or Melphalan administration, gathering of S-phase cells was observed, but these changes appeared later with K18 than with Melphalan. This result showed that the effect of K18 was produced by the alkylating activity of Melphalan which was combined with immunoglobulin. For clinical application, K18 was administered to cancer patients at a dose of 60-90 mg every day. Two cases of good response were achieved. No side effect was observed. This remarkable efficacy and low degree of side effects in clinical application is probably due to the higher affinity and accumulation of K18 in the tumor region. K18 is a useful new drug for clinical application alone, or in combination with other chemotherapeutic drugs.

Animals↗
PubMed1986

[A case of primary ureteral carcinoma in the duplicated renal pelvis and ureter diagnosed by transurethral uretero-renoscopy].

We present a case of primary ureteral carcinoma in the duplicated renal pelvis and ureter diagnosed by transurethral uretero-renoscopy. The case was of a 78-year-old man with the complaint of sudden asymptomatic macrohematuria. An excretory urogram strongly suggested the presence of duplication of the right collecting system, and cystoscopy revealed a gross hematuria from the right ureteral orifice. A retrograde ureteropyelogram revealed incomplete duplication of the right renal pelvis and ureter fused at about the ureter crossing over the iliac vessels, and a polyp-like filling defect in the lower segment of duplicated ureter at about 4 cm from the fusion of the ureters. Transurethral uretero-renoscopy was employed to investigate the filling defect, and a papillary tumor extended into the lower segment of duplicated ureter was revealed. Tumor was resected by a rigid operating instrument under transurethral uretero-renoscopy. The pathological diagnosis was grade I-transitional cell carcinoma of the ureter, so that right total nephroureterectomy with partial cystectomy was carried out subsequently. Surgical specimen after right total nephroureterectomy with partial cystectomy showed no other tumor in the pelvis or ureter macroscopically, and histopathological studies of surgical specimens were no evidence of malignancy. We believe that transurethral uretero-renoscopy significantly increases the diagnostic accuracy in determining the nature of upper urinary tract lesions, and this procedure is indispensable in the diagnosis of ureteral tumors. The present case was the 7th case of primary ureteral carcinoma in the duplicated renal pelvis and ureter in the Japanese literature.

Aged↗
PubMed1985

Purification of opioid-binding materials from rat brain.

Opiate receptors were solubilized from rat brain with digitonin and treated with an affinity resin, AH-Sepharose coupled with [D-Ala2, Leu5]enkephalin. The eluted materials from the resin with the opioid peptide had opioid-binding activity. Based on the protein content of the purified materials, 450-fold purification over the solubilized receptors was achieved. Analyses by gel electrophoresis revealed that the purified materials were rich in one polypeptide with a molecular weight of 62,000.

Animals↗
PubMed1985

[Concentration level of latamoxef sodium (LMOX) in prostatic tissue].

The concentration of latamoxef sodium (LMOX) in serum and prostatic tissue was evaluated. Thirty seven patients with benign prostatic hypertrophy were given 1 g LMOX intravenously prior to prostatectomy. Prostatic tissue and blood were sampled at 1, 2 or 3 hrs after administration of LMOX. The concentration of LMOX in prostatic tissue was 17.4 +/- 3.4 micrograms/g tissue, 11.1 +/- 1.3 microgram/g tissue and 8.9 +/- 1.3 microgram/g tissue 1, 2 and 3 hrs after injection, respectively (mean +/- S.E.). The penetration ratio of LMOX (concentration in tissue/in serum) to prostate was 30-45%. Therefore, the 80% MIC of LMOX is lower than 8 micrograms/ml for most gram negative bacteria, the results suggest that LMOX is very effective against prostatitis caused by these organisms.

Aged↗
PubMed1985

[Implant operation of penile inflatable prosthesis for a patient with erectile impotence due to radical cystectomy: a case report].

The hydraulically inflatable penile prosthesis consists of an inflate-deflate pump, a storage reservoir and paired cylinders and it is able to provided a more physiologically functional penis. We report our surgical experience of implant of an inflatable penile prosthesis for postoperative impotence. The patient was a 51-year-old male with impotence, who had received radical cystectomy and ilial conduit formation for transitional cell carcinoma of the bladder (G3, pT3NoMo.) 9 months earlier. There was nothing particular except for the postoperative status on physical, laboratory and X-ray examinations. He was diagnosed to have organic impotence (vasculogenic) by sexual functional tests. Auto-injection of the corpus cavernosum with papaverine HCl was attempted but an erection was not induced by the injection. So, implant operation of an inflatable penile prosthesis was performed by the lower abdominal approach. His postoperative course was uneventable and practice to deflate and inflate the cylinders was started at 2 weeks after the operation and he was able to have sexual intercourse at 5 weeks after the surgery. Now (3 months after the operation), he can have sexual intercourse with an orgasm but without ejaculation and he and his wife are both satisfied with the postoperative status.

Carcinoma, Transitional Cell↗
PubMed1985

[Immunohistochemical demonstration of carcinoembryonic antigen (CEA) and ABO (H) blood group antigens on tissue sections of urinary bladder tumors].

The carcinoembryonic antigen (CEA) and ABO (H) blood group antigens of 47 bladder tumors were investigated by immunohistochemistry (CEA by peroxidase-antiperoxidase method and blood group antigens by Avidin-biotin-peroxidase complex method) and the results were compared with the histopathological diagnosis. Twenty five percent of the bladder tumors had CEA-positive tumor cells. They were found in 11.1% of pT1 cases, in 12.5% of pT2 cases, 70.0% of pT3 cases and 50.0% of pT4 cases and were also found in 13.3% of grade I cases, in 50.0% of grade II cases and in 40.0% of grade III cases. On the other hand, positive blood group antigen cells were found in 85.2% of pT1 cases, 50.0% of pT2 cases, 20.0% of pT3 cases and 50.0% of the pT4 cases and also in 80.0% of the grade I cases, 41.1% of the grade II cases, and 20.0% of the grade III cases. A statistical difference was seen between the low stage (pT1) and high stage cases (pT2-pT4) and between the low grade (G-I) and high grade cases (G-II, III) (P less than 0.01) for both CEA and the blood group antigens which were statistically related (P less than 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)

ABO Blood-Group System↗
PubMed1985

[Two cases of giant vesical diverticulum in females].

Two rare cases of giant vesical diverticulum without infravesical obstruction in female are presented. The first case, a 58-year-old female, was admitted because of diverticulum with continuous urinary infection, which was diagnosed by urological examinations for hematuria. The second case, a 58-year-old female, was admitted with the diagnosis of diverticulum, which was done by the gynecologist during laparotomy for the suspicion of a right ovarian cyst. Neither patient complained of difficulty in urination in spite of remarkable residual urine and no neurologic abnormalities were detected. The cystograms of the two cases revealed over-goose-egg sized diverticula and vesical capacity was over 800 ml, in both cases. The other urograms and endoscopy showed no evidence of vesical trabeculation or any obstructive changes of bladder neck or urethra in either case. Therefore, mucosal diverticulectomy in the first and total diverticulectomy in the second, was performed for the residual urine. Each surgical specimen showed the muscle layer of the walls. In the post-operative course, urination was improved in spite of the underactive detrusor pattern of the cystometry in the first case. But residual urine volume was not decreased after the operation in the second case. These two cases seem to be congenital vesical diverticulum. But it is hard to rule out the possibility of secondary diverticulum due to neurogenic disorders of urinary bladder.

Cystoscopy↗
PubMed1985

[Experience in using a ureterorenoscope in 14 cases].

We carried out studies using an ureterorenoscope in 14 cases, including 13 patients suffering from an ureteral stone. The stone was smaller than 1 cm and existed in lower and middle portion. The other patient had a foreign substance, which was a double J-stent catheter that had been passed into the middle portion of the ureter accidentally. Initially, the size and course of ureter, the position of stone and foreign substance were ascertained using a retrograde pyelogram. The patients were placed in a lithotomy position under spinal anesthesia or epidural anesthesia. The ureterorenoscope was inserted into the bladder through urethra and the instrument was further extended through ureteral orifice and ureteral lumen to the position of the calculus or foreign substance while observing the procedure. Once the calculus or foreign substance was observed, a 4F Pfister-Schwartz stone retriever or forceps was inserted through working channel. After coming in contact with the stone or foreign substance, the matter was removed while observing it with the scope. The foreign substance and 77% of the stones observed were removed by this procedure and the usefulness of the ureterorenoscope was successfully demonstrated in the cases. However, on the other hand, of these 14 cases, 2 (14%) experienced ureteral injury. In one case, the ureter was penetrated by the scope; and in another, injury occurred during the biopsy of the ureteral wall. Therefore, it is our opinion that more careful use of the ureterorenoscope is necessary to prevent these types of injuries.

Adult↗
PubMed1984

Fine structure of the lutein cell in the house musk shrew, Suncus murinus.

The ultrastructure of lutein cells during pregnancy and the post partum period was examined by transmission electron microscopy in the house musk shrew, Suncus murinus. Lutein cells on day 13 of pregnancy contained an extensive system of anastomosing tubules or cisternae of smooth ER and many enlarged mitochondria with numerous tubulovesicular cristae. From day 13 on, the number of small granules, possibly lysosomes, increased gradually. Between day 20 and 25, the loss of smooth ER began and elongated, or flattened mitochondria increased. Regressing lutein cells observed after parturition were characterized by abundant large dense bodies, bizarre mitochondria and a decrease in the amount of smooth ER. Unusual forms of mitochondria were always observed after day 5 of pregnancy. Two types could be distinguished; one, found frequently in the second third of pregnancy, was ring-, disc-, cup- or dumb-bell-shaped with tubulovesicular cristae, and the other, found exclusively in the last third of pregnancy and after parturition, was elongated, flattened and sometimes twisted. The paucity of lipid droplets was a characteristic feature of the lutein cells of this species. The significance of these ultrastructural changes of cellular organelles is discussed in relation to the ovarian and plasma levels of progesterone.

Animals↗
PubMed1984

X-ray microanalysis of monoaminergic terminals in the nucleus tegmentalis dorsalis of the chicken.

X-ray microanalysis after aldehyde-chromate-dichromate treatment served to confirm the presence of monoaminergic terminals in the nucleus tegmentalis dorsalis (NTD) of the chicken. The monoaminergic terminals were represented as neuronal elements with electron-dense vesicles (EDVs) of several different shapes as seen in Eponembedded semi-thin sections. Conventional electron microscopic observations of the adjacent ultra-thin sections showed the EDVs to be comprised of spherical medium-sized (about 80 nm in diameter), large dense-cored (about 120 nm) and elongated granular vesicles (100-220 nm) in the same nerve varicosities. It is probable that the NTD, being a center of catecholaminergic efferent projections, may also receive direct monoaminergic inputs from an unknown area of the brain and/or from recurrent collaterals of the same catecholamine-containing neurons in the NTD.

Animals↗
PubMed1984

A photoaffinity reagent to label the opiate receptors of guinea pig ileum and mouse vas deferens.

An enkephalin derivative, [D-Ala2,Leu5]enkephalin N-[(2-nitro-4-azidophenyl)amino]ethylamide, has been synthesized as a photoaffinity label for the opiate receptor. This compound retains the full biological activity of [D-Ala2,Leu5]enkephalin in guinea pig ileum and mouse vas deferens tests with IC50 values of 4.4 and 2.6 nM, respectively, and inhibits the binding of [3H]naloxone to rat brain membrane preparation with an IC50 value of 2.5 nM. Photolysis of a muscle strip of the guinea pig ileum or of the mouse vas deferens in the presence of the peptide derivative caused irreversible inhibition of electrically stimulated contractions with high efficiencies (80 and 66%, respectively), while the inhibitory effect in the dark was fully reversed by washing. This irreversible inhibition during photolysis was completely prevented by the presence of [D-Ala2,Leu5]enkephalin. These results demonstrate that [D-Ala2,Leu5]-enkephalin N-[(2-nitro-4-azidophenyl)amino]ethylamide is a prominent candidate as a photoaffinity label for the opiate receptor.

Affinity Labels↗