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Biomedical subjects

T Fukusato

Publications and source records attributed to T Fukusato.

At least 37 records · Page 2Linked to original sources
PubMed1995

Bone marrow cellularity: quantification by chemical-shift misregistration in magnetic resonance imaging and comparison with histomorphometrical techniques.

The accuracy of a technique that uses chemical-shift misregistration in magnetic resonance (MR) imaging to quantify vertebral bone marrow cellularity was tested on cadaveric specimens. In order to estimate the cellularity of 11 unfixed vertebral bodies, mid-sagittal MR images were obtained using a 1.5T magnet with a proton-density-weighted spin-echo sequence (repetition time/echo time, 2500/22 ms) and a narrow bandwidth. These values were subsequently compared to the histomorphometrical values for cellularity, amount of trabecular bone and deposited iron. The image-derived values for cellularity (VI, %) correlated well with values determined by histomorphometry (VH, %) (VI = 21.2 + 0.72 VH, r = 0.92) regardless of the presence of trabecular bone and small amount of stored iron in the specimens. This simple technique may be applicable in the estimation of marrow cellularity.

Adult↗
PubMed1995

Extrahepatic portal-systemic encephalopathy without portal hypertension.

We report three cases of extrahepatic portal-systemic encephalopathy (EHPSE), two of which did not show portal hypertension (PH) and in the other of which precirrhotic state with chronic active hepatitis was detected at liver biopsy. Our review of the literature regarding EHPSE without PH leads us to conclude that a part of the shunts is congenitally or spontaneously formed, that PH is not always essential to shunt formation. Since the clinical symptoms most often do not manifest until middle age, cases with only mild disturbance of consciousness are not easily distinguished from dementia. Furthermore, all reported patients underwent shunt closure followed by marked improvement of clinical symptoms and uneventful postoperative course. EHPSE without PH which can be corrected by shunt closure should always be differentiated from EHPSE with PH.

Aged↗
PubMed1995

Creatine phosphokinase-linked immunoglobulin associated with hypokalemic myopathy.

We present three women with hypokalemic myopathy in whom serum creatine phosphokinase (CPK) was bound to serum immunoglobulin (macro-CPK). In all three cases, CPK isozyme electrophoresis demonstrated an extra CPK band between CPK-MM and CPK-MB. The bound immunoglobulins were identified as IgA/A-kappa and lambda, IgA-lambda and IgA-kappa and lambda, respectively. In all cases, histological examination of the biopsied muscles revealed necrotic and/or regenerating fibers. In each patient, potassium replacement therapy returned the macro-CPK as well as the marked elevations of serum muscle enzymes to normal. Taken together with other recent reports, our findings suggested that CPK-immunoglobulin binding may be related to the emergence of pathophysiology of hypokalemic myopathy.

Aged↗
PubMed1994

Epididymis metastasis from colon carcinoma: a case report and a review of the Japanese literature.

A 56-year-old Japanese man in whom a descending colon carcinoma had been resected underwent a high orchitectomy for metastatic epididymis from the colon. Metastatic carcinoma from the digestive organs to the spermatic cord (SC) and/or the intrascrotal contents (ISC) is rare. Fifty-four Japanese patients with metastasis from the digestive organs to the SC and/or the ISC were analyzed. The most frequent primary site was the stomach and the most frequent metastatic site was the spermatic cord. Our patient was the first to exhibit metastasis from the colon to the epididymis. A primary site was identified in 24 patients subsequent to the metastatic tumor. Twenty patients had other organ metastasis. The prognosis for SC and/or ISC metastasis patients is poor. Our patients has survived for 18 months following his orchitectomy with no other organ metastasis (31 months after the primary operation). In our patient, monoclonal estrogen receptor staining was negative; however, further study must be undertaken.

Carcinoma↗
PubMed1990

Immunohistochemical and ultrastructural localization of epidermal growth factor receptor in human liver and hepatocellular carcinoma tissues.

Expression of epidermal growth factor receptor (EGF-R) in human hepatocellular carcinoma (HCC), hepatoblastoma and non-cancerous liver tissues was investigated immunohistochemically in order to evaluate the possible role of EGF-R expression in neoplastic transformation of hepatocytes. Immunoreactive EGF-R molecules were identified on frozen sections by means of the avidin-biotin immunoperoxidase complex technique using a monoclonal antibody recognizing an epitope of the external domain of human EGF-R. Linear positive staining was present on the surface of carcinoma cells in one hepatoblastoma and in 9 of 11 HCCs. In addition, an enhanced level of surface EGF-R expression was observed on the tumor cells in 9 of 12 cases in comparison with that on hepatocytes in surrounding non-cancerous liver tissue, which in most cases showed chronic inflammation, hepatocyte injury or regeneration. No positive staining in the form of coalescent cytoplasmic granules was present in HCC or hepatoblastoma cells, nor in the cytoplasm of hepatocytes in normal or non-cancerous diseased liver tissue. Little or no reactivity was present on the surface membrane of hepatocytes in the normal liver tissues of 8 control cases. Furthermore, immunoelectron microscopy revealed the localization of this immunoreactive EGF-R molecule on the plasma membrane. Considering that the functional form of EGF-R could be localized on the plasma membrane, the enhanced expression of immunoreactive EGF-R on the tumor cell surface demonstrated here may suggest a possible role of EGF-R in the development or progression of human HCC as well as in hepatocyte regeneration.

Carcinoma, Hepatocellular↗
PubMed1990

Paraneoplastic encephalomyelitis. An autopsy case with encephalitis principally confined to the thalamus and associated with pulmonary and gastric cancers.

We report an autopsy case of encephalitis principally confined to the bilateral thalamus and associated with pulmonary and gastric cancers. An 81-year-old man exhibiting behavioral abnormalities and progressive somnolence died of pneumonia 11 months after onset. Because the patient had lung cancer and no definite cause of the encephalitis was found, this case was categorized as one of paraneoplastic encephalomyelitis. This may be the first reported case of this condition in which the central nervous system (CNS) lesion was located predominantly in the thalamus.

Aged↗
PubMed1990

[Two cases of advanced gastric cancer with diffuse carcinomatosis of the bone marrow showing a widespread osteoplastic bone metastasis].

Reported are 2 cases of an advanced gastric cancer with a "diffuse carcinomatosis of the bone marrow" (DCMB) showing a widespread osteoplastic bone metastasis. In a DCBM, a widespread invasion of cancer cells to the bone marrow occurs, causing leukoerythroblastosis, anemia, and disseminated intravascular coagulation. The local reaction in the bone, namely being osteoplastic or osteolytic, has not been discussed in previous papers. Our cases were characterized by an extremely high serum alkaline phosphatase, a "superscan" view of the bone scintigram, and a diffuse osteoplastic bone metastasis with an osteoid increase. Thus we think there are DCBM subtypes with a diffuse osteoplastic bone metastasis.

Adenocarcinoma, Mucinous↗
PubMed1989

Acute necrotizing encephalopathy with widespread edematous lesions of symmetrical distribution.

A 67-year-old Japanese woman with liver cirrhosis was affected by an unusual acute progressive encephalopathy, presenting mental confusion and slurred speech as its initial symptoms. She died in profound coma, following the entire course of 17 days. Autopsy disclosed bilateral symmetrical, widespread, edematous and necrotic lesions, their centers being located in the basal ganglia, diencephalon and midbrain, and their peripheries expanding into the cerebral white matter, cerebellum, pons and medulla. Diapedesis of erythrocytes and serum plasma was conspicuous, in contrast to paucity of capillary proliferation. Although the lesions were somewhat similar to those of Wernicke's and Leigh's encephalopathies, they were considered to be representative of a more acute metabolic disorder distinct from the latter conditions.

Acute Disease↗
PubMed1989

Age and sex differences in bone metastasis of hepatocellular carcinoma in Japanese autopsy cases.

Metastatic patterns of hepatocellular carcinoma (HCC) in 6997 autopsy cases recorded in the Annual of the Pathological Autopsy Cases in Japan from 1981 to 1984 were analyzed by using a computer. Significant association of the metastatic pattern of hepatocellular carcinoma with age and sex was shown after separating patients into different age groups in pack years. It was especially worthy of notice that bone metastasis decreased in an age-dependent manner only in men within the widest age range (P less than 0.001) and was significantly prevalent in men in their forties and fifties (P = 0.012). In addition, in 350 cases of hepatocellular carcinoma autopsied at Tokyo University Hospital during 15 years from 1971 to 1985, it was suggested that bone metastasis might be prevalent in male patients with liver cirrhosis although significant relationship between bone metastasis and hepatitis B virus infection or alcohol abuse was not shown. These results might indicate possible effects of sex differences on the metastatic pattern of hepatocellular carcinoma, particularly bone metastasis, as well as on the incidence of the cancer.

Adult↗
PubMed1988

Synergistic effect of 5-azacytidine and gamma-interferon or dimethyl sulfoxide on expression of HLA class I antigens by PLC-PRF-5 cells.

In order to elucidate the mechanism by which HLA antigens expression is induced or enhanced on the injured or transformed hepatocytes, we have made in vitro studies using human hepatic tumor-derived cell lines as a model system. In the present study, PLC-PRF-5 cells that have the integrated form of hepatitis B virus genome in DNA were treated with 5-azacytidine (5-azaC) in combination with gamma-interferon (IFN-gamma) or dimethyl sulfoxide (DMSO). HLA antigens on the cell surface were quantitated by using a modified cell-ELISA method. As a result, it was demonstrated that DMSO- or IFN-gamma-treatment enhanced expression of HLA class I antigens on the cell surface. In addition, enhanced expression of the antigens on PLC-PRF-5 cells treated with 5-azaC in combination with IFN-gamma or DMSO represented a synergistic effect of these inducers on HLA class I antigens expression although no changes in HLA antigens expression were induced after 5-azaC-treatment alone in short-term experiments. Furthermore, an indirect immunofluorescent analysis of hepatitis B surface antigen on the cells demonstrated increased expression of the antigen after 5-azaC-treatment alone. HLA class II antigens and hepatitis B core antigen were not induced even after those treatments and also not after a long-term experiment. These results might indicate possible modulation of HLA class I and hepatitis B virus antigens expression on the cultured cells by a DNA hypomethylating agent, 5-azaC.

Antibodies, Monoclonal↗
PubMed1986

Expression of HLA class I antigens on hepatocytes in liver disease.

Recent studies have demonstrated aberrant expression and topographical heterogeneity of HLA Class I and Class II antigens in tissues of patients with certain immunologic or neoplastic diseases. Current information about the expression of HLA antigens by normal and diseased hepatocytes is controversial. We analyzed the HLA antigenic profile of 4 normal fetal livers, 5 normal adult livers, 7 cases of chronic hepatitis B virus (HBV) infection, 14 cases of cirrhosis of various etiologies, 11 hepatic neoplasms, and 5 continuous cell lines derived from hepatic tumors. The specimens were tested by the indirect fluorescent antibody method with a panel of monoclonal antibodies to distinct monomorphic determinants of HLA Class I and Class II antigens and to beta 2-microglobulin. HLA Cells I antigens were not detected on normal fetal and adult hepatocytes, but were displayed on the plasma membrane of hepatocytes in the majority of all hepatic diseases tested and of the 5 hepatic tumor cell lines. There was a significant correlation between the expression of HLA Class I antigens on hepatocytes and the intensity of intralobular inflammation. Double immunofluorescent staining of livers infected with hepatitis B virus demonstrated simultaneous expression of HLA Class I antigens and HBsAg or HBcAg only in a small percentage of hepatocytes, suggesting lack of a specific association between HLA Class I and these viral antigens. HLA Class II antigens were not detected on hepatocytes from any of the liver diseases tested but were expressed by one of the 5 liver carcinoma cell lines analyzed. These findings confirm that HLA Class I antigens are not detectable within the limits of several immunohistochemical methods on normal hepatocytes and suggest that injury by a variety of factors directly or indirectly leads to induction of these antigens on the plasma membrane of hepatocytes.

Adolescent↗
PubMed1984

Radioimmunometric assay for hepatitis B surface antigen on the surface of infected cells.

It has been difficult to differentiate hepatitis B surface antigen (HBsAg) on the cell surface from intracellular or intramembranous HBsAg that is not exposed to the host immune response. We describe here a radioimmunometric assay for HBsAg on the cell surface using HBsAg producing hepatocellular carcinoma cell lines and fibroblasts transfected with cloned hepatitis B virus DNA as models. The assay is sensitive, specific, simple and takes approximately 1 1/2 h. The procedure may be modified to compare quantitatively cell surface with intracellular HBsAg and to demonstrate other cell associated hepatitis B virus antigens such as HBcAg and HBeAg.

Animals↗
PubMed1984

A pathological study of fulminant hepatic disease.

Among 4,030 autopsies, 67 cases of fulminant hepatic failure were selected and studied from a pathological viewpoint. No sex difference in incidence was seen and the patients' age ranged very widely from 3 months to 78 years, showing comparatively high risk in the 20 s and 30 s in both sexes. Hepatitis virus was the most common cause, followed by toxic chemicals, shock, etc. Histopathological study revealed severe and extensive hepatic cell necrosis as the most conspicuous and common change of the liver, which was pathomorphologically quite similar to that experimentally produced by focal intravascular coagulation in rabbit. This evidence, as well as pathological findings of the other organs obtained at autopsy and the patients' reported clinical course, including laboratory data, were consistent with our proposal of a pathological disease entity, fulminant liver disease, which corresponds to the clinical symptom-complex known as fulminant hepatic failure. It is suggested that the characteristic feature of acute, severe and extensive hepatic cell necrosis which is seen in such cases is probably a result of an anoxic state caused in most instances by intrahepatic circulatory disturbances. Besides, we consider that fulminant liver disease can be regarded as a group of systemic diseases with the main focus of illness in the liver, rather than a specific disease of a single organ, the liver.

Adolescent↗