Biomedical subjects

T G Bromage

Publications and source records attributed to T G Bromage.

21 records · Page 2Linked to original sources

PubMed1980

A brief review of cartilage and controlling factors in chondrocranial morphogenesis.

It is evident that chondrocyte determination is an example of cellular aging. This possible leads to the erroneous view that chondrocytes have a "weak" genetic control mechanism. If such was the case, chondrocranial growth and development would be a very "shaky" process indeed. It is recognized however that chondrocranial growth is under predominantly genetic and tissue interaction influence especially during the early phase of growth. These control factors plus other epigenetic and local environmental influences are responsible for the harmonious growth of the chondrocranium.

Animals↗

PubMedDate not supplied

Correlative light and backscattered electron microscopy of bone--Part I: Specimen preparation methods.

A method for preparing nondecalcified bone and tooth specimens for imaging by both light microscopy (LM) and backscattered electron microscopy in the scanning electron microscope (BSE-SEM) is presented. Bone blocks are embedded in a polymethylmethacrylate (PMMA) mixture and mounted on glass slides using components of a light-cured dental adhesive system. This method of slide preparation allows correlative studies to be carried out between different microscopy modes, using the same histologic section. It also represents a large time savings relative to other mounting methods whose media require long cure times.

Adhesives↗

PubMedDate not supplied

Correlative light and backscattered electron microscopy of bone--part II: automated image analysis.

Detailed studies of biological phenomena often involve multiple microscopy and imaging modes and media. For bone biology, various forms of light and electron microscopy are used to study the microscopic structure of bone. Integrating information from the different sources is necessary to understand how different aspects of the bone structure interact. To accomplish this, methods were developed to prepare and image thin sections for correlative light microscopy (LM) and backscattered electron imaging in the scanning electron microscope (BSE-SEM). Images of the same fields of view may then be analyzed for degrees of relationships between specimen features not observed by LM or SEM alone. These methods are applied here to study possible associations between the degree of bone mineralization and pattern of collagen fiber orientation in the mid-shaft of the human femur. The "relational images" obtained allow us to examine the relationship between these two variables, both objectively and quantitatively.

Femur↗