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T G Gross

Publications and source records attributed to T G Gross.

41 records · Page 3Linked to original sources
PubMed1986

Transfer of the Epstein-Barr virus (EBV) DNA fragment coding for EBNA-1, the putative transforming antigen of EBV, into normal human lymphocytes: gene expression without cell transformation.

Fresh human lymphocytes were transfected with cloned EBV DNA fragments containing the coding exon for EBNA-1. DNA-loaded reconstituted Sendai Virus envelopes (RSVE) were used for efficient gene transfer. EBNA was detected by immunofluorescence in 1-5% of cells transfected with either the cloned BamHl K fragment of EBV DNA (5.1 kb) or the recombinant plasmid pSV3neoEBNA1, containing only the 2.0kb EBNA-1 coding exon. EBV-specific mRNA was detected by hybridization up to 14 days after DNA transfer. Quantitation of mRNA by laser densitometry revealed that the transcription level was similar to that obtained after infection with an intact virus. However, no effect on cellular proliferation was observed by (3H)-thymidine incorporation assays, and transformation was not achieved. We conclude that though EBNA-1 may be necessary for cellular immortalization by EBV, it alone is not sufficient.

Antigens, Viral↗
PubMed1984

Infection of mouse lymphocytes by Epstein-Barr virus. II. Stimulation of cellular DNA synthesis by EBV in the absence of EBNA induction and cell transformation.

Epstein-Barr virus (EBV) of the transforming and nontransforming strains induced transient stimulation of cellular DNA synthesis during lytic infection of normal mouse lymphocytes. In contrast to human B lymphocytes, the action of both EBV strains in mouse cells was additive. The nontransforming P3HR-1 virus had no cytotoxic effect on mouse lymphocytes. The EBV-infected, stimulated mouse lymphocytes did not express EB virus-determined nuclear antigen and do not grow into immortalized cell lines. The cells expressed EBV-determined early and virus capsid antigens. These results suggest that the stimulation of cellular DNA synthesis by EBV is independent of EBNA synthesis and cell transformation.

Animals↗
PubMedDate not supplied

From Burkitt's lymphoma to chronic active Epstein-Barr virus (EBV) infection: an expanding spectrum of EBV-associated diseases.

Epstein-Barr virus (FBV) is one of 8 known human herpesviruses. EBV infection usually occurs in early childhood and is subclinical. However, primary infection in adolescence or adulthood causes infectious mononucleosis in approximately half of infected individuals. Recently, the spectrum of human diseases associated with EBV injection has increased, primarily due to methodological advances in EBV detection. Initially, EBV was isolated from a cultured Burkitt lymphoma cell line, and has been felt to be etiologically linked to the development of Burkitt lymphoma, as well as other human malignancies. This review mainly focuses on pathogenetic mechanisms, many of which remain enigmatic, for the various human diseases, which are considered to be associated with EBV injection.

Adolescent↗