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Biomedical subjects

T G Orlova

Publications and source records attributed to T G Orlova.

At least 19 recordsLinked to original sources
PubMed1992

[Effect of mytilane, a bioglycan from the mussel Crenomytilus grayanus, on the course and outcome of experimental flu infection].

Materials characterizing the antiviral activity of Mytilane, manifested by the protection of 50-60% of mice infected with the lethal dose of influenza virus and by a decrease in the severity of pathological changes in the lungs of mice, are presented. The inhibiting activity of Mytilane with respect to the reproduction of influenza virus in vivo and in vitro under experimental conditions is demonstrated.

Adjuvants, Immunologic↗
PubMed1991

[Use of alpha interferon preparations in acute lymphoblastic leukemia in children during remission].

The Soviet alpha-interferon drugs (human leukocytic interferon, reaferon) were used in the program of the treatment of children with acute lymphoblastic leukemia (during remission). The treatment was found to be more effective with an increase of its duration, relapse-free course of the disease (69.4-82.3%) and survival (70.1-93.1%) of patients with an unfavourable disease prognosis (over 5 years).

Adolescent↗
PubMed1990

Production of alpha-interferon by human leukocytes.

We examined the capacity of human leukocytes to produce alpha-interferon subtypes following viral induction. It was shown that the beta-propiolactone inactivated viral inductor stimulated the production of a complete set of native alpha-interferon subtypes. Acid treatment of native interferon at pH 2.0 inactivated the acid-labile portion of alpha-interferon. Its exposure to 37 degrees C for 5 days and repeated acid treatment at pH 2.0 resulted in additional inactivation of some interferon pool fractions. The acid-labile subtypes of human alpha-interferon were formed in a nonadherent mononuclear cell fraction.

Humans↗
PubMed1979

Comparative study on the anticellular and antiviral effects of interferon and the haemopoietic stem cell inhibition factor.

Pretreatment with crude interferon preparations obtained from suspension cultures of bone marrow, spleen and thymus cells or from mouse L-cell cultures or with mouse serum interferon preparations did not change the colony-forming activity of bone marrow cells on syngeneic transplantation to lethally irradiated mice. Preparations of L-cell culture interferon, dialysed and purified by carboxymethyl-Sephadex (G-25) column chromatography, showed an inhibitory effect on exogenous colony formation by bone marrow cells. The results suggested the presence in crude interferon preparations of a substance either inhibiting the anticellular effect of interferon or stimulating colony formation. The factor produced by thymus cells following their treatment with antilymphocyte serum inhibited colony formation by bone marrow cells and, unlike interferon, possessed no antiviral activity when tested in cell cultures.

Animals↗
PubMed1977

[Antiviral activity of preparations of RNA isolated from cells treated with interferon (messenger RNA for an antiviral protein?)].

Chick embryo cultures treated with interferon yielded a biologically active RNA which, upon inoculation into chick embryo cells, created an antiviral condition in them. The level of vesicular stomatitis virus reproduction in such cells was 2-30% of that observed in the cells treated with control RNA. The maximum activity of the experimental RNA was seen 3 hours after the treatment with interferon.

Animals↗
PubMed1977

Translation by bacterial cells of messenger RNA for interferon of animal origin.

Escherichia coli, strain AB 1157, cells are capable of translating human, mouse, and chicken messenter RNA for interferon with production of interferon of the corresponding specifity. This translation occurs in the presence of serum. The activity of the resulting interferon decreased in parallel to dilution of the original mRNA preparation, upon multiple ulitization of the mRNA solution, as well as upon reduction of the interferon- producing activity of cells-donors of mRNA due to prolonged storage of the cells. Unlike animal cells, the bacteria do not require pre-treatment with actinomycin D. The interferon translated by bacteria is inactivated by trypsin and resistant to ribonuclease.

Animals↗
PubMed1977

Infectivity of DNA recovered from cells persistently infected with SV5 paramyxovirus.

Infectivity of DNA isolated from L cells chronically infected with SV5 paramyxovirus was demonstrated by inoculation of continuous RH and HEp-2 cells. Infectivity of the DNA was completely abolished by treatment with deoxyribonuclease or by alkaline hydrolysis but did not change after treatment with ribonuclease and specific anti SV5 serum. The virus obtained as a result of transfection caused haemadsorption in susceptible cells and was neutralized by specific antiserum like the prototype SV5 strain.

Animals↗
PubMed1976

The dynamics of production and the sensitivity to cycloheximide and actinomycin D of interferon-inducing and interferon messenger RNA.

In the process of virus-induced interferon production, two kinds of RNA appear in the cells. One of them induces production by the recipient cells of interferon with the species-specificity of the latter cells (interferon-inducing RNA), and the other is translated by the recipient cells pre-treated with actinomycin D into interferon with the species-specificity of the donor cells of RNA (interferon mRNA). The interferon-inducing RNA appears 20-30 minutes after virus induction and shows maximal activity after 1 hour. Its formation is not influenced by cycloheximide or actinomycin D. This RNA is assumed to be a transcriptive intermediate form of viral RNA. Interferon mRNA appears in the cells 1 hour after virus induction and shows maximal activity after 6-8 hours. Its synthesis is inhibited by cycloheximide and actinomycin D.

Animals↗
PubMed1975

[Bone marrow interferon].

The nucleated cells of the bone marrow of mouse, rat, guinea pig, chick, cattle and humans proved to be capable of producing interferon in vitro following induction with the Newcastle disease virus. The production of interferon by these cells was characterized by high stability. The bone marrow interferon was not inferior in its activity to the corresponding interferon prepared with the blood leukocytes or splenic cells.

Animals↗
PubMed1975

Neuraminidase activity of influenza virus-infected cells: localization and properties.

Infection of chick embryo cell (CEC) cultures with influenza virus results in the appearance of neuraminidase activity lost by the cells as a result of cultivation. Neuraminidase activity is associated mainly with the lysomal cell fraction. Different distribution of neuraminidase activity in lysosomes with various densities, different reaction to sodium ethylene diamine tetracetate (EDTA) and differenct optimal pH suggest that at early stages of viral infection the cell enzyme is activated and by the 7th hour of infection viral neuraminidase is synthesized.

Acid Phosphatase↗
PubMedDate not supplied

[Production of virus-induced and mitogen-induced interferons by canine cells].

Cells of monocytic-phagocytic series and immunocompetent cells derived from dogs: bone marrow, leukocytes, spleen, thymus, lymph nodes (with the exception of the liver) were found to be capable of producing interferon (IFn) in vitro in response to inoculation of viral and non-viral inducers. This capacity is manifested in different ways: thymus and lymph node cells were less active interferon producers. Studies of physicochemical properties of the resulting interferons confirmed the belonging of the virus-induced IFn to alpha-interferons, PHA-induced IFn to immune alpha-IFn. The above-mentioned refers both to bone marrow and leukocyte interferons.

Animals↗