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Biomedical subjects

T Gerdes

Publications and source records attributed to T Gerdes.

27 records · Page 2Linked to original sources
PubMed1991

Molluscum contagiosum in three horses.

Suspected molluscum contagiosum was diagnosed in 3 horses in the Chingola district of Zambia. The horses were found to be suffering from a slow progressive skin disease with lesions on the chest, shoulders, inner and lateral aspects of the fore- and hindlimbs, the face, fetlocks, pasterns and on the lateral surfaces of the body. The lesions varied from 4 to 20mm in diameter, were hairless but covered by soft keratin projections which, when removed, left a raw elevated base tightly adherent to the epidermis. These lesions bled profusely when the animals were groomed. Older lesions were well circumscribed, raised above the surface, devoid of hair and after removal of grey-white keratin flakes, had a depigmented waxy appearance. Microscopically cytoplasmic inclusions containing many pox virions were found. Attempts at culturing the virus were unsuccessful.

Animals↗
PubMed1991

Molluscum contagiosum in a horse.

An adult stallion presented with a severe papular dermatitis of especially the neck, chest and genitalia. A marked scrotal oedema was present. Histopathological examination of skin biopsies, revealed the presence of numerous intracytoplasmic molluscum bodies in areas of focal epidermal hyperplasia. Electron microscopical examination showed the presence of typical pox virions in affected epidermal cells. Attempts at viral isolation were unsuccessful. This is believed to be the first reported case of molluscum contagiosum in a horse in the Republic of South Africa.

Animals↗
PubMed1987

Clinical performance of a system for semiautomated chromosome analysis.

Until recently equipment for automated chromosome analysis has not been used for routine purposes in clinical cytogenetic laboratories. During a 3 1/2-year period the chromosome laboratory of Rigshospitalet has tested the Magiscan chromosome system under routine conditions and performed the first evaluation of its clinical performance. The system consists of an image processor with a light pen for manual interaction connected to a hard-copy printer and a microscope with a TV camera and a motorized scanning stage for eight slides. Automated metaphase finding takes place without operator assistance. An operator is involved in the analysis after the metaphases are located. Using two of these complete systems, we have performed a total of 4,691 chromosome analyses comprising a count of 10 metaphases, of which three were "eyeball" karyotyped and one was "machine" karyotyped. Presently, two-thirds of our prenatal analyses (amniotic-cell cultures) are carried out with these two machines. A third Magiscan system without scanning stage is used as a "karyotyping-only" system to produce hard-copy karyograms in those cases in which metaphases are manually located and counted in the microscope. Since the end of 1984, 4,773 additional machine karyograms have been produced with this system. With a complete system, a prenatal analysis can be carried out in an average of 35 min. The average time for a machine karyotype is 7 min. Since 1984 the productivity of the laboratory has increased 17%-20% without enlarging the staff.

Female↗
PubMed1986

Automatic classification of chromosomes as part of a routine system for clinical analysis.

A procedure for automatic classification of G-banded human chromosomes has been implemented on a semiautomated system for routine clinical analysis. Chromosomes represented by their density profiles are described by so-called weighted density distributions (WDDs) by application of a number of weighting functions and classified by a parametric discriminant analysis. During 16 mo of routine use of the system, 2,794 metaphases (127,925 chromosomes) from amniotic fluid have been karyotyped by the system with an error rate of 8-9%. This corresponds to 4-5 errors per metaphase. These errors can immediately be corrected by the operator on a displayed karyogram with a light pen.

Amniotic Fluid↗
PubMed1982

[Influence of the mushroom poison gyromitrin on the lipids of rat liver (author's transl)].

Gyromitrin, the main poison of the false morel Gyromitra esculenta, causes enlargement of the liver in rats. The content of triglycerides and total cholesterol were increased in the liver homogenate and microsomal fraction but not in the mitochondrial fraction. On the other hand the content of phospholipids was found to be decreased. The enhanced incorporation of [14c]-acetate into the lipids of rat livers after gyromitrin indicates an increased rate of lipids synthesis. The diminution of the phospholipids is caused mainly by a loss of phosphatidyl ethanolamine (PE) although after a short pulse with [14C]-ethanolamine the specific radioactivity of the isolated phosphatidyl ethanolamine corresponded to that of the controls. Since the content of phosphatidylcholine (PC) remained unchanged, the PC/PE-ratio was shifted from 2.3 to 4.5. These effects were reversible within 48 h. Inhibition of the synthesis of highly unsaturated fatty acids by the microsomal monoxygenase system is discussed as the primary reason for the above observations this inhibition possibly being due to active metabolites of gyromitrin which destroy the monoxygenases.

Acetaldehyde↗
PubMed1981

Automatic chromosome analysis. II. Karyotyping of banded human chromosomes using band transition sequences.

Human chromosomes, represented by band transition sequences, chromosome area, centromeric index by area and centromeric index by density, were karyotyped by computer. A reference set of chromosomes provided frequencies of occurrence of each density class and difference class of the band transition sequence as well as of each of the three global features. The karyotyping program was designed to handle all metaphases, even those from which severely bent and overlapped chromosomes were excluded. In one experiment, 21 metaphases were karyotyped on the basis of a reference set and the results were compared with earlier results of visual analysis of band transition profiles developed from band transition sequences: 0.8% errors were made in the visual experiment and 1.4% errors were made in the computer based experiment. In a second experiment, 179 metaphases were divided into reference and test sets and karyotyped by computer with an error rate of 3.4%. By further analysis it was found that metaphases with many misclassified chromosomes could often be automatically distinguished from metaphases with few errors. Thus by automatic rejection of 7% of the metaphases the error rate could be reduced to 2.6%. The computer program for chromosome karyotyping will now be implemented in a semi-automatic system for practical clinical chromosome analysis.

Chromosome Banding↗
PubMedDate not supplied

Increasing identification and referral of panic disorder over the past decade.

The study's objective was to contrast the prevalence, phenomenology, and medical care utilization for panic disorder from 1980 to 1990. All psychiatric consultations from a university consultation service from the years 1980, 1985, and 1990 were located (N = 2,400). Patients meeting DSM-III-R panic disorder criteria were selected for chart review. Variations in demographics, comorbidity, prior medical evaluations, and referral patterns were analyzed. The prevalence rate for consultations meeting panic disorder criteria has increased (2.5% in 1980, 4.2% in 1985, and 5.1% in 1990; chi 2 = 7.5, P = 0.024). Referring physicians more frequently noted panic attacks in 1990 (5% in 1980, 21% in 1985, and 59% in 1990; chi 2 = 21.2, P = 0.0001). A summary measure of medical care utilization revealed no significant interval change. Panic disorder is being recognized and referred more frequently by medical physicians since the publication of DSM-III and DSM-III-R. However, delay of diagnosis and high medical care utilization remain significant problems.

Adult↗
PubMedDate not supplied

Induction of apoptosis in human microvascular endothelial cells by divalent cobalt ions. Evidence for integrin-mediated signaling via the cytoskeleton.

Wound healing following implantation is characterized by an acute inflammatory reaction and a subsequent reorganizing phase in which angiogenesis is involved. Endothelial cells (EC) participate in both inflammation and angiogenesis. Thus, the effects on functions of EC exerted by implanted materials could affect the progression of wound healing. The corrosion of metallic implants can cause high concentrations of heavy metal ions in the peri-implant tissues. The purpose of the present study was to test the effects of possible corrosion products on the function and viability of human EC in vitro. Long-term exposure of EC to CoCl2 and NiCl2 (3 days, 0.7 mM) leads to a decrease of cell number and changes in cellular morphology. However, the morphological changes between CoCl2- and NiCl2-treated cells differ significantly. The changed morphology of CoCl2-treated EC and the fragmented DNA pattern indicates apoptosis. Nickel-treated cells demonstrated necrosis. The activity of integrins was tested by an assay of cellular adhesion on collagen-coated surfaces. It was shown that the number of adherent cells significantly decreased upon exposure to CoCl2. Our studies suggest that induction of cell death in EC upon exposure to CoCl2 could be attributed to impaired integrin signaling, which leads to a damaged cytoskeleton and culminates in apoptosis.

Journal Article↗