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T Gilliam

Publications and source records attributed to T Gilliam.

5 recordsLinked to original sources
PubMed1997

Two forms of Wilson disease protein produced by alternative splicing are localized in distinct cellular compartments.

Copper is an essential trace element in prokaryotes and eukaryotes and is strictly regulated by biological mechanisms. Menkes and Wilson diseases are human disorders that arise from disruption of the normal process of copper export from the cytosol to the extracellular environment. Recently a gene for Wilson disease (WD)(also named the ATP7B gene) was cloned. This gene encodes a copper transporter of the P-type ATPase. We prepared monoclonal and polyclonal anti-(WD protein) antibodies and characterized the full-length WD protein as well as a shorter form that is produced by alternative splicing in the human brain. We found that the WD protein is localized mainly in the Golgi apparatus, whereas the shorter form is present in the cytosol. These results suggest that the alternative WD proteins act as key regulators of copper metabolism, perhaps by performing distinct roles in the intracellular transport and export of copper.

Adenosine Triphosphatases↗
PubMed1993

Adherence of Helicobacter pylori to cultured human gastric epithelial cells.

Experiments were performed to demonstrate that adherence of Helicobacter pylori to gastric epithelial cells causes alterations in the cell cytoskeleton. H. pylori intimately attached to cultured human gastric epithelial cells on small cellular projections, while there was no intimate association of H. pylori with cultured human esophageal epithelial cells. Fluorescein-conjugated phalloidin staining of gastric epithelial cells showed that H. pylori adherence stimulated actin polymerization; this stimulation was not observed with esophageal cells. Also, this organism's selectivity for gastric mucosa was supported by rare binding of bacteria to esophageal epithelial cells and gastric fibroblasts.

Actins↗
PubMed1988

When the patient requires observation not hospitalization.

Developing alternative health care delivery systems that successfully meet the needs of multiple consumers in a cost-efficient manner is essential. Minor revisions of long-standing programs are frequently inadequate in the face of escalating demands for a more efficient health care system. The SSU Observation Area has met the primary objectives by providing a quality, cost-efficient alternative to the use of existing inpatient and emergency department services. Alternatives, such as the SSU, require three major components to succeed: A risk-taking environment. Innovative nursing leaders, especially at the department head level. Staff nurses who demonstrate flexibility, enthusiasm, and pride in expanding their professional roles. The SSU was not developed without difficulty or some conflict. As the program was developing, full-time night coverage was not warranted so an on-call system was used. Increased use of the SSU resulted in the on-call person frequently being called in rather than just occasionally being called. Staff discontent grew as this continued. Additional night staff were added and use continued to increase, which ensured financial viability. Nursing leaders are developing alternatives in every setting. As we learn from each other, the opportunities for change grows.

Child↗