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T Goldman

Publications and source records attributed to T Goldman.

At least 19 recordsLinked to original sources
PubMed2005

Platelet glycoprotein I(b)alpha and integrin alpha2 beta1 polymorphisms: gene frequencies and linkage disequilibrium in a population diversity panel.

Genetic variants in the GP1BA and ITGA2 genes have been proposed as potential modifiers for arterial vascular disease and bleeding disorders. Since ancestry may play an important role in the prevalence of these variants, we sought to determine their allele frequency and linkage disequilibrium in a collection of 1064 DNA samples from 51 ethnic groups. We studied haplotypes of ITGA2 defined by single nucleotide substitutions at positions -52, 807, and 1648, and GP1BA variants defined by sequence changes in positions -5 (Kozak), 1018 (T145M, HPA-2) and 1285 (VNTR A, B, C and D). Frequency of haplotypes of ITGA2 showed considerable variation across the different groups, with a higher prevalence of the haplotype -52C or T/807C/1648A observed in African compared with caucasian and Asian populations. The haplotypes 52C/807T/1648A and -52T/807T/1648A were not observed in caucasians or South Americans. While relative frequencies of the GP1BA Kozak alleles were comparable across groups, the methionine allele (HPA-2b) showed a higher frequency in Africa (0.26) than in the other groups. We also observed a high prevalence of the VNTR B allele in the African and Israeli populations. Haplotype analysis revealed incomplete linkage disequilibrium between the HPA-2 and VNTR alleles. Incorporation of GP1BA variants into the set of SNPs already genotyped by the HapMap project disrupted the pre-existing haplotype block. These data provide a valuable resource for optimal selection of variants best tailored for association studies of vascular disease or bleeding disorders when examining individuals of different ancestral origins.

Alleles↗
PubMed2004

Breakpoint of a balanced translocation (X:14) (q27.1;q32.3) in a girl with severe hemophilia B maps proximal to the factor IX gene.

Hemophilia B is an X-linked bleeding disorder caused by the deficiency of coagulation factor (F)IX, with an estimated prevalence of 1 in 30 000 male births. It is almost exclusively seen in males with rare exceptions. We report a girl who was diagnosed with severe (<1%) FIX deficiency at 4 months of age. Cytogenetic studies in the patient showed a balanced translocation between one of the X-chromosomes and chromosome 14, with breakpoints at bands Xq27.1 and 14q32.3. Both parents were found to have normal chromosomes. Late replication studies by incorporation of 5-bromodeoxyuridine showed non-random inactivation of the normal X-chromosome, a phenomenon frequently seen in balanced X/autosome translocations. To map the breakpoint, fluorescent in-situ hybridization was performed. A PAC DNA probe, RP6-88D7 (which contains the FIX gene) hybridized only on the normal chromosome X as well as onto the derivative 14. Using a PAC DNA probe, RP11-963P9 that is located proximal to the FIX gene, we obtained signals on the normal and derivative X and also on the derivative 14. We conclude that the breakpoint is located within the DNA sequence of this clone mapping proximal to the FIX gene. Since the FIX gene seems to be intact in the derivative 14, the breakpoint may affect an upstream regulatory sequence that subjects the gene to position effect variegation (PEV).

Child↗
PubMed2001

Relativistic symmetry suppresses quark spin-orbit splitting.

Experimental data indicate small spin-orbit splittings in hadrons. For heavy-light mesons we identify a relativistic symmetry that suppresses these splittings. We suggest an experimental test in electron-positron annihilation. Furthermore, we argue that the dynamics necessary for this symmetry are possible in QCD.

Journal Article↗
PubMed1997

Mapping of microsatellite markers developed from a flow-sorted swine chromosome 6 library.

Swine Chromosome (Chr) 6-enriched libraries, generated with size-fractionated DNA isolated from chromosomes sorted by flow cytometry, have been used to develop new Chr 6 microsatellite markers. Chromosome isolation procedures were established to reproducibly prepare high quality chromosomes from phytohemagglutinin (PHA)-stimulated swine peripheral blood lymphocytes and to sort individual chromosomes after staining with Hoechst 33258 and chromomycin A3. Chromosome purity was verified by specific staining of swine Chr 6 with fluorescence in situ hybridization (FISH) by use of painting probes generated by degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR) amplification of as few as 300 sorted Chr 6. For library construction, DNA was extracted from flow-sorted pools representing Chr 6, amplified, size selected for fragments from 300 to 700 bp, and ligated into pBluescript SK II+ or Lambda ZAP Express. The libraries were then screened with a radiolabeled poly-(dCA) DNA probe. Of 107 (CA)n repeat-containing clones verified by sequencing, 21 were polymorphic and used to genotype the University of Illinois swine reference families. Linkage analysis was then performed with CRIMAP 2.4 (LOD > 3.0), and the results showed that 15 of the microsatellites mapped to swine Chr 6. At least three of these new markers map to locations where there were gaps in the consensus Chr 6 map. Another four markers, because of their PIC values, should provide more informative markers in other areas of the map. Most of the new markers can also be used for automated genotyping with fluorescent labeling. This set of 15 new Chr 6 markers will, therefore, be useful in helping to define QTL associated with swine Chr 6.

Animals↗
PubMed1995

The self-employment option for people with disabilities: a case study of 'AHVA' desk top publishing company.

From the above results of the evaluation survey it can be concluded that the participants of the 'AHVA' desktop publishing company, who are with multiple and physical disabilities and who have initiated and run their own enterprise, perceived their first year of operation as beneficial. They mastered new technical skills, learned new work-related behaviours, expanded their interpersonal competencies and felt personal growth. They all developed high motivation to make their operation successful. The case study of 'AHVA' desktop publishing company, initiated and run by a group of multiply and physically disable persons, is unique and demonstrates the positive trend for the future. It is an example of personal and group commitment to build a competitive enterprise. It is self determination rather than the actual outcome of services provided, that is the driving force behind their efforts. The team members demonstrated that persons with disabilities can indeed take charge of their lives, be masters of their own destinies, and lead a productive and meaningful life. Here, inclusion is not a one-sided process in which service providers and rehabilitation experts try to integrate persons with disabilities into the community and regular employment. Inclusion is to integrate persons with disabilities into the community and regular employment. Inclusion is achieved here by the self initiative of a group of persons with disabilities. They have created an extraordinary enterprise according to their own special needs. Non-disabled people interact with them on an equal level, as partners in a business, sharing mutual interests of quality production.(ABSTRACT TRUNCATED AT 250 WORDS)

Behavior↗
PubMed1994

Analyses of anti-human CD monoclonal antibodies for cross reactions with swine cell antigens.

For the First International Swine CD Workshop an effort was made to include commercially available anti-human CD monoclonal antibodies (mAb) that had been prescreened to determine whether they were cross-reactive with swine cell CD antigens. Thus before the actual workshop began, ten companies were contacted to determine whether they would participate in this effort. Four companies (Dako Corporation, Carpenteria, CA; T Cell Sciences, Cambridge, MA; Bioproducts for Science, Indianapolis, IN; PharMingen, San Diego, CA) provided a total of 38 mAb for tests of cross-reactions with swine cell antigens. Two companies (Coulter, Hialeah, FL and Becton Dickinson, San Jose, CA) declined to participate because of the low likelihood of positive cross-reactions. Several other companies (Behringewerke, Marburg, Germany; Boehringer Mannheim, Indianapolis, IN; Biosource International, Camarillo, CA; Applied Immune Sciences, Menlo Park, CA) were interested but either had problems in preparing the mAb in time for US importation or in providing the quantities necessary free to the workshop. The studies described below verify that few anti-human CD mAb exhibit consistent, predictable cross-reactions on pig cells; of the seven mAb that showed reactivity on swine cells only one mAb, Dako's anti-CD18, showed consistent reactivity with every pig tested and at the expected intensity level.

Animals↗
PubMed1994

Summary of workshop findings for porcine T-lymphocyte antigens.

Fifty-four mAb preselected in the first round of the first porcine CD workshop for their possible reactivity with T-lymphocyte specific antigens and/or activation antigens were further analysed in a second round. PBMC, thymocytes and nylon-wool purified T lymphocytes derived from peripheral blood, mesenteric lymph nodes and spleen served as target cells for flow cytometric analyses. For the classification of activation antigens several experiments were performed with activated, mitogen-stimulated T lymphocytes and long-term T-lymphocyte cultures. Out of the 54 mAb, 35 mAb could be distributed to six different CD clusters and two swine workshop clusters (SWC). Five mAb could be distributed to the porcine CD2, four mAb to the CD4. Six mAb seemed to recognize the porcine CD5 and two mAb the porcine CD6 analogue. Six mAb were directed against the porcine CD8, whereas two different epitopes could be defined. One mAb was directed against the porcine CD25 analogue. Nine mAb could be clustered to the SWC1, defining an antigen on T lymphocytes and cells of the myeloic linage. Two mAb with high T-cell specificity were clustered to the SWC2.

Animals↗
PubMed1994

Analyses of monoclonal antibodies reactive with porcine CD5.

Among all monoclonal antibodies (mAbs) analyzed in the first porcine CD workshop, six mAbs showed reactivity to the porcine CD5 antigen (workshop mAbs 067, 068, 069, 070, 071 and 119). Because of lack of immunoprecipitation studies for five (067, 068, 069, 070 and 071) out of the six mAbs, only one mAb (119) could be definitely characterized as mAb against the monomeric 63 kDa porcine CD5 antigen. The five other mAbs included in this cluster are characterized by an identical labelling pattern in FCM and competition of the CD5 epitope recognized by mAb 119. These mAbs were allocated to the wCD5 subcluster.

Animals↗
PubMed1994

Analyses of monoclonal antibodies reactive with porcine CD6.

Amongst the monoclonal antibodies (mAbs) submitted to the first porcine CD workshop, two mAbs (workshop numbers 055 and 120) could be identified to recognize the porcine CD6 analogue. Both mAbs seemed to be highly T-cell specific and showed neither reactivity with cells of the myeloic lineage nor with B lymphocytes. The observed molecular mass of the antigen precipitated by mAb 120 of 110 kDa confirmed this classification. Without molecular analyses of the antigen recognized by mAb 055, but similar staining pattern in FCM compared with 120, mAb 055 was allocated to the wCD6 subcluster.

Animals↗
PubMed1994

Analyses of mAb reactive with porcine CD8.

Among all mAb submitted to the first porcine CD workshop, based on FCM analyses six mAb could be identified to recognize the porcine CD8 analogue (workshop Nos. 004, 051, 052, 053, 108 and 109). In immunoprecipitation studies three mAb (Nos. 004, 108 and 109) recognized an antigen with an apparent molecular mass of about 35 kDa under reducing conditions and about 70 kDa under non-reducing conditions. The molecular masses of the antigens recognized by the three other mAb (Nos. 051, 052 and 053) are still unknown. Epitope analyses performed by blocking experiments led to the determination of two CD8 epitopes: CD8a and CD8b. CD8a is recognized by mAb Nos. 004, 051 and 052, and CD8b by Nos. 053, 108 and 109.

Animals↗
PubMed1994

Analysis of mAb reactive with the porcine SWC1.

Among 54 mAb determined to be reactive with porcine T lymphocytes and/or activation antigens, eight mAb (workshop Nos. 005, 031, 080, 091, 092, 093, 094 and 110) derived from different laboratories grouped together in the T11 cluster and were ordered into the SWC1. One mAb (No. 111) which belong also to this group was lost during the workshop. The SWC1 antigen is a molecule expressed on the majority of leukocytes, resting T lymphocytes, monocytes and granulocytes, but not on B lymphocytes. On T lymphocytes it is down-regulated after activation. The molecular mass of the antigen is unknown. Epitope analyses revealed that seven out of the nine mAb recognized similar epitopes on the SWC1 molecule.

Animals↗
PubMed1993

Trichinella spiralis: genetic basis and kinetics of the anti-encysted muscle larval response in miniature swine.

Primary-infected swine lymphocyte antigen (SLA) inbred miniature swine, NIH minipigs, of the SLAa/a haplotype (aa) display markedly reduced Trichinella spiralis-encysted muscle larval (ML) burdens 6 weeks after homologous challenge and are therefore referred to as responders against encysted T. spiralis ML. To investigate the kinetics and genetics of the anti-encysted ML response, and in an attempt to ascertain the mechanisms underlying this phenomenon, NIH minipigs of multiple SLA haplotypes, designated either as ax (aa, ac, ad, af, ag, dh and hh) or non-ax (cc, cd, and dd), received a primary inoculation of 300 T. spiralis ML followed by a challenge inoculation of 10,000 ML 6 or 17 weeks later. Pigs were examined at necropsy and at Weeks 1, 2, 3.5, and 6 after challenge. Statistical cluster analyses, based on final ML burdens, performed on 129 pigs in 13 trials, revealed that 47% of primary-plus-challenged ax pigs exhibited responder phenotype as early as 1-2 weeks after T. spiralis challenge whereas this response was manifested in only 8% of non-ax pigs. No correlation was observed between responder phenotype and T. spiralis-specific blastogenesis, cell expression of class I or class II SLA molecules, lymphoid cell subsets, adult worm numbers, or fecundity. Although some ax responder pigs exhibited a localized inflammatory response with a prominent eosinophilic infiltrate surrounding degenerating encysted ML, no correlation was observed between responder phenotype and either peripheral blood eosinophilia or the ability of eosinophil-enriched cell populations from responder pigs to adhere to or destroy encysted ML in vitro.

Animals↗