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Biomedical subjects

T H Khan

Publications and source records attributed to T H Khan.

9 recordsLinked to original sources
PubMed1999

Novel inhibitors of carboxypeptidase G2 (CPG2): potential use in antibody-directed enzyme prodrug therapy.

The design and synthesis of potent thiocarbamate inhibitors for carboxypeptidase G2 are described. The best thiocarbamate inhibitor N-(p-methoxybenzenethiocarbonyl)amino-L-glutamic acid 6d, chosen for preliminary investigations of in vitro antibody-directed enzyme prodrug therapy (ADEPT), abrogated the cytotoxicity of a combination of A5B7-carboxypeptidase G2 conjugate and prodrug PGP (N-p-{N,N-bis (2-chloroethyl)amino}phenoxycarbonyl-L-glutamate) toward LS174T cells. This is the first report of a small-molecule enzyme inhibitor proposed for use in conjunction with the ADEPT approach.

Aniline Mustard↗
PubMed1996

Blood pressure and its correlates in the population of Multan, Pakistan.

In a randomly selected sample of 1579 individuals (male = 1334 and female = 245), aging from 14 to 65 years and representing all the socioeconomic groups of Multan, age (Yr), height (cm), weight (kg), BMI (kg/ml) and blood pressure (mmHg) were recorded. Height was measured on a Holtain portable stadiometer, weight on beam scale and blood pressure was recorded with sphygmomanometer as per auscultatory method. The correlation coefficient between different independent (age, height, weight and BMI) and dependent (SBP, DBP and MAP) parameters in male, female and total population were calculated and strong association between different parameters was observed (p < 0.001 or p < 0.05). The regression equations (simple and multiple) were worked out. The regression coefficient of different independent parameters with dependent parameters are higher in female except for height and age-weight and age-BMI had higher contribution in female as compared with that of male.

Adolescent↗
PubMed1994

Blood pressure distribution in a school age population of Quetta, Pakistan.

Standardized measurements of blood pressure, height and weight were obtained in 8-11 years old children, attending school in Quetta, Balochistan, Pakistan. The sample consisted of 427 individuals (219 boys and 208 girls). The blood pressure was measured by auscultatory method. To calculate the body mass index (BMI), the weight (kg) of each subject was divided by the square of the subject's height. Association between various variables and blood pressure was judged through correlation coefficient. The net and joint effect of selected variables on blood pressure variability was calculated using simple/multiple regression. The correlation coefficients with that of systolic blood pressure were 0.365, 0.427 and 0.341 whereas these were 0.397, 0.430 and 0.320 for diastolic blood pressure. Height had a regression coefficient of 0.475 mmHg/cm with systolic pressure and 0.419 mmHg/cm with diastolic blood pressure. Weight had a regression coefficient of 0.878 mmHg/kg and 0.717 mmHg/kg with systolic and diastolic pressure respectively whereas BMI had a regression coefficient of 2.35 mmHg/BMI unit with systolic pressure and 1.79 mmHg/BMI unit with diastolic blood pressure.

Blood Pressure↗
PubMed1992

Kinetics of Bacillus cereus phosphatidylinositol-specific phospholipase C with thiophosphate and fluorescent analogs of phosphatidylinositol.

Thiophosphate analogs (C-S-P bond) of phosphatidylinositol (Cn-thio-PI: racemic hexadecyl-, dodecyl-, and octylthiophosphoryl-1-myo-inositol) and a fluorescent analog (pyrene-PI: rac-4-(1-pyreno)-butylphosphoryl-1-myo-inositol) were all substrates for phosphatidylinositol-specific phospholipase C from Bacillus cereus. Hydrolysis of thio-PI was followed by coupling the production of alkylthiol to a disulfide interchange reaction with dithiobispyridine. Hydrolysis of pyrene-PI was followed using a HPLC-based assay with fluorescence detection. The activity of PI-PLC with thio-PI analogs showed an interfacial effect. C16-Thio-PI, which had a critical micelle concentration (CMC) of 7 microM, gave a hyperbolic activity versus concentration curve between 0 and 2 mM, while C8-thio-PI, which had a CMC above 10 mM, showed very low activity which increased greatly upon introduction of an interface in mixed micelles with hexadecylphosphocholine (HDPC). Pyrene-PI, which aggregates above 0.3 mM, gave a sigmoidal activity curve with much higher activity above the CMC. All three thio-PI homologs as mixed micelles with HDPC gave hyperbolic activity curves with PI-PLC that were a function of bulk concentration of substrate at constant surface concentration and surface concentration of substrate at constant bulk concentration. The maximal activity of PI-PLC with pure C16-thio-PI micelles was 6.25 mumol min-1 mg-1, while that with pyrene-PI was estimated to be 68 mumol min-1 mg-1. With pure C16-thio-PI micelles, 0.022 mM substrate gave half Vmax, similar to that in mixed micelles with HDPC.

Bacillus cereus↗
PubMed1992

Arthroscopic resection of chondroblastoma of the knee.

We present a case of chondroblastoma of the upper tibia treated by complete arthroscopic excision. The diagnostic difficulties raised by the case are discussed. This method of treatment has not, to our knowledge, been reported in the literature.

Adolescent↗