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T H Stanton

Publications and source records attributed to T H Stanton.

25 records · Page 2Linked to original sources
PubMed1976

Quantitative isolation of mouse thymus leukemia antigen, TL.

This report describes the purification of TL from papain digests of a tumor line (ASL1) and of an established cell line (L251A). Through the use of gel filtration and ion-exchange chromatography, the TL was purified approximately 100-fold with respect to the original digest. It was noted that the TL isolated from ASL1 had a specific activity 1.4 times higher than that isolated from L251A. The reason for this anomaly is unexplained. However, this work indicates that classical methods of protein chemistry can be used in the purification of these membrane components which are present in only small amounts on the cell surface.

Animals↗
PubMed1975

Purification of murine thymus leukemia antigen (TL). A quantitative assessment of limited proteolysis.

The murine thymus leukemia antigen (TL) has been solubilized from the tumor ASL1 and from an established cell line ASL1W, by papain digestion. When a 15-min digest was chromatographed on Sephadex G-200, two peaks of TL activity were eluted with apparent molecular weights of approximately 58,000 and 31,000. Chromatography of a 30-min digest under the same conditions resulted in elution of a single peak of activity with an apparent molecular weight of 58,000. Additional purification was carried out on the 58,000 molecular weight material by absorption to, and elution from DEAE-cellulose. The combination of gel filtration and ion exchange chromatography resulted in approximately a 150-fold purification.

Animals↗
PubMed1975

Adaptation to tissue culture of the murine leukemia ASL1, a high producer of TL (thymus leukemia) antigen.

ASL1, a murine thymus derived leukemia, was adapted to tissue culture and its suitability as a source of TL antigens was examined. Tissue culture medium was developed in which the cell grew with a mean doubling time of 12.5 h and 95% viability. The quantity of antigen expressed by the cells was determined by inhibition of lysis of 51Cr labelled leukemic cells meadiated by anti-TL 1,2,3 serum. Significant quantities of the antigens were retained by the cells during exponential growth in vitro.

Animals↗
PubMed1975

Subfragments of the papain solubilized TL antigen.

TL antigen was solubilized from the tumor ASLI (TL. 1,2,3) by papain digestion. The subfragments of 125I-labeled TL were examined by two methods. The first involved immune precipitation followed by electrophoresis on SDS-acrylamide gels. This treatment yielded three bands of molecular weight 39,000 and 19,000, as well as material which migrated with the tracking dye. In the second procedure the papain digested material was partially purified on Sephadex G-200. The active fraction from G-200 was labeled with 125Iodine, mixed with alloantiserum and rechromatographed on G-200. The isolated immune complexes were boiled in SDS and 2-mercaptoethanol, then separated on a SDS-Sephadex G-150 column. Two radioactive peaks were eluted indicating an absence of the 19,000 m.w. component following the latter method of purification.

Animals↗
PubMedDate not supplied

Fraud-and-abuse enforcement in Medicare: finding middle ground.

Medicare fraud and abuse cost billions of dollars each year. Yet Congress is considering legislation to hamper enforcement. Providers' anger over enforcement led to a congressional compromise several years ago to limit excesses. If providers and their advocates were to hobble enforcement, this could provoke a backlash. Instead, the existing compromise should be strengthened to accommodate legitimate provider concerns while allowing enforcement against major fraud and abuse. Government should further confine, structure, and check its discretion in applying the False Claims Act. Enhancing the Health Care Financing Administration's capacity to ensure that contractors pay claims properly would remove additional points of friction.

Centers for Medicare and Medicaid Services, U.S.↗