Biomedical subjects

T Habu

Publications and source records attributed to T Habu.

12 recordsLinked to original sources

PubMed1990

Protein secretion by rat submandibular glands in response to isoproterenol, alpha-methylnoradrenaline and clonidine during aging.

Changes in salivary volumes and the three types of proteins secreted by the submandibular salivary gland (SMG) of male rats at 3.5, 5.5, 8, 12, 13, 14, 15, 19, 21 and 24 months of age in response to the beta 1-, alpha 1- and alpha 2-adrenoceptor agonists, isoproterenol (IPR), alpha-methylnoradrenaline (alpha-mNA) and clonidine (Clonid), were studied and compared by measuring the weight and by isoelectric focusing electrophoresis with the Phast System on both the gradient pH 3.5-5 and 3.5-9 gels with silver staining. A protein (protein A, tentatively termed in this study) purified by FPLC from saliva elicited by IPR was also analyzed by SDS-polyacrylamide gel electrophoresis, the immuno-thermoblotting method, carbohydrate determination and neuraminidase treatment. Unexpected findings were observed that salivary volumes, but not the protein concentration, were substantially increased by Clonid-, but not IPR-, stimulation with ages up to 24 months of age and that the three types of proteins elicited by each agonist were different during aging. The gamma-type of proteins elicited by Clonid was not greatly changed during aging, whereas several proteins at about neutral pI in the alpha-type, elicited by alpha-mNA, at 5.5 to 21 months of age and a protein A in the beta-type, elicited by IPR, at 13 to 24 months of age were greatly increased. This protein A without any carbohydrate and sialic acid, located only in the acinar cells, but not in any duct system, had a molecular weight of 16,000 and a pI of 4.05. We conclude that the secretory function of the SMG in the aged animals is in general little changed.

Aging↗

PubMed1990

Changes in protein secretion by rat submandibular glands in response to isoproterenol, alpha-methylnoradrenaline, and clonidine during post-natal development.

We studied developmental changes in salivary volumes and proteins secreted by the submandibular glands of male rats at weekly intervals from two to ten weeks of age in response to the beta 1-, alpha 1-, and alpha 2-adrenoceptor agonists, isoproterenol (IPR), alpha-methylnoradrenaline (alpha-mNA), and clonidine (Clonid). The types of proteins in saliva samples were determined and compared by isoelectric-focusing electrophoresis with the Phast system in both the gradient pH -3.5-to-5 and pH-3.5-to-9 gels by means of silver staining. Salivary volume and protein concentration in saliva samples elicited by IPR and alpha-mNA were positively related to the weight of the submandibular glands up to six or seven weeks of age, whereas in saliva elicited by Clonid, no relation was found in the protein concentration [corrected]. The isoelectric-focusing electrophoretic patterns of proteins secreted by the glands in response to three stimuli were different from each other during post-natal development. Within one stimulation, differences were also observed at two and three weeks of age for Clonid, and from seven weeks of age for the three stimuli, respectively. The alpha-type proteins, but not the beta-type proteins, were very similar to those in extracts from glands of rats at seven weeks of age. Almost all of the alpha-type proteins, but not the beta-type proteins, reacted with antibodies to two proteases. We conclude that functional maturation precedes morphological maturation in the submandibular glands of rats.

Age Factors↗

PubMed1990

[Supplemental studies on self-curing resins. 2. On polymerizing temperature of reline materials].

The objective of this study was to find the best way to keep down the peak temperature of self-curing reline materials. The experiments were done by using three kinds of self-curing reline materials (Rebaron, REBASE and KOOLiner) and three kinds of the reline materials of different thickness (1.5mm, 2mm and 3mm) under three different intraoral conditions. When these reline materials cured under rinsing the mouth with cold water (18 degrees C), the data analysis of these reline materials showed a considerable reduction in the peak curing temperatures clinical acceptance. The results obtained were as follows: 1. Both extraoral and interoral curing tests showed that the thicker the material is the higher the peak temperature becomes. 2. Highly significant differences in time to the peak temperature were found among the three kinds of reline materials. 3. Dangerously high temperature was found in polymerizing of the thickest material, 3mm.

Denture Liners↗

PubMed1988

Age changes in secretory function of male and female rat parotid glands in response to methoxamine and pilocarpine.

Saliva secreted in response to methoxamine and pilocarpine was collected from the cannulated ducts of both parotid glands of male and female rats at weekly age intervals from three to 10 weeks, and at 3.5, 8, and 15 months of age. It was analyzed for the concentrations of protein, potassium, calcium, inorganic phosphate, and for amylase activity. The type of protein were determined electrophoretically, and an amino acid analysis of the total protein was also carried out. The wet weights of the glands increased substantially up to eight weeks of age, then reached almost plateau values, and finally tended to decrease at 15 months of age in both sexes. The salivary volumes secreted in response to methoxamine and pilocarpine were positively correlated with the parotid gland weights in both sexes. The concentrations of protein, potassium, and inorganic phosphate were inversely related to the salivary flow rates only at relatively low rates of flow. The amylase activity was positively correlated with the concentration of protein, independent of the nature of the stimulus, age, and sex. With methoxamine as a stimulus, the amylase activity was positively correlated with the concentration of calcium, independent of age and sex. The types of protein and amino acid concentrations were independent of the nature of the stimulus, age, and sex up to 15 months of age. However, in parotid saliva of several rats at 8 and 15 months of age, unusual proteins were observed electrophoretically, independent of the nature of the stimulus and sex.

Aging↗

PubMed1988