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T Hatayama

Publications and source records attributed to T Hatayama.

At least 91 records · Page 5Linked to original sources
PubMed1991

Different induction of 70,000-Da heat shock protein and metallothionein in HeLa cells by copper.

To clarify the physiological roles of heat shock proteins induced by copper, we studied the synthesis of these proteins and metallothionein, as well as the level and nature of copper incorporated into HeLa cells. Incubation in medium containing 200 microM cupric sulfate and above induced the synthesis of 70,000-Da heat shock protein (hsp70) in these cells. However, the synthesis of hsp70 did not increase in the presence of less than 200 microM cupric sulfate. On the other hand, the synthesis of metallothionein increased due to 100 microM cupric sulfate. The uptake of copper into the cells depended on the cupric sulfate concentration in the medium. To analyze the nature of the intracellular copper, cell extracts were separated by gel filtration chromatography into three fractions: the high molecular weight, metallothionein, and low molecular weight fractions. No copper was found in the low molecular weight fraction of control cells, but appeared distinctly at 200 microM cupric sulfate and above. Copper in the high molecular weight fraction also began to increase at 200 microM cupric sulfate and above, whereas in the metallothionein fraction it began to increase even at 50 to 100 microM cupric sulfate. Furthermore, inhibition of cell growth was also observed at 200 microM cupric sulfate and above but not at 100 microM and below.(ABSTRACT TRUNCATED AT 250 WORDS)

Cell Division↗
PubMed1991

Vestibular nerve injury as a complication of microvascular decompression.

We report a case of hemifacial spasm in which hearing was well preserved after microvascular decompression, but the vestibular nerve was injured selectively. We review the pathophysiological mechanisms relevant to this complication in the light of results of animal experimental studies we have conducted. In addition, we discuss the clinical significance of this particular type of cranial nerve injury.

Arteries↗
PubMed1991

[A resected case of renal cell carcinoma with metastasis to pancreas].

We report a case of renal cell carcinoma with metastasis to the pancreas, treated by radical nephrectomy and total pancreatectomy. A 56-year-old man visited our hospital because of macrohematuria and right low backache. An intravenous pyelography, ultrasonography and a CT scan of the abdomen revealed right renal tumor at the upper portion, about 11 cm in diameter, but no abnormal findings of the pancreas. Aortic and celiac angiograms demonstrated multifocal lesions, 1 or 2 cm in size, compatible with a metastatic tumor in the region of the pancreas. The patient underwent right radical nephrectomy and open biopsy of the pancreas. The right renal tumor was histologically revealed to be renal cell carcinoma without nodal or venous extension. Histological examination of the pancreas biopsy specimen confirmed it to be a renal cell carcinoma metastatic to the pancreas. Therefore, he underwent total pancreatectomy 1 month after the previous surgery. Three months after the second surgery, a CT scan of the brain revealed metastasis to the pituitary gland. He is still under therapy.

Carcinoma, Renal Cell↗
PubMed1991

Induction of heat shock protein and thermal sensitivity of mammalian cells maintained at low culture temperature.

Effects of low culture temperature on the induction of heat shock proteins in FM3A cells by a heat shock and on the thermal sensitivity of the cells were examined. FM3A cells maintained at 33 degrees C could not induce hsp70 during continuous heating or after a short heat shock at either 39, 42, or 45 degrees C, although FM3A cells maintained at a normal culture temperature of 37 degrees C can induce the synthesis of hsp70. Furthermore, the cells maintained at 33 degrees C were more sensitive to the subsequent heat shock than the cells maintained at 37 degrees C. Thus, the culture temperature of the mammalian cells may be an important factor for the induction of hsp70, and hsp70 may play an important role to protect or repair the thermal damage of cells.

Animals↗
PubMed1991

[A case of pelvic lipomatosis].

This is a report of the fifth case of pelvic lipomatosis in Japan. A 52-year-old man presented himself in our hospital with a complaint of left lower abdominal pain on August 28, 1988. At that time, physical examination was unremarkable with the exception of mild obesity. The excretory urogram and retrograde pyelogram revealed left hydroureteronephrosis with tapering of the left lower ureter. Urethrocystogram showed an elongated posterior urethra with anterior displacement and elevation of the bladder. Computed tomography revealed excess of diffuse fatty tissue in the pelvic space with bladder deformity and rectal compression. Pelvic arteriogram demonstrated no neovascularity. A diagnosis of pelvic lipomatosis was established. He lost 6 kg by diet therapy. Left lower abdominal pain disappeared, but excretory urogram after eight months showed no changes.

Humans↗
PubMed1991

[A case of emphysematous cystitis with familial amyloidosis].

This is a report of the thirteenth known case in Japan of emphysematous cystitis. A 70-year-old man visited our hospital because of pollakisuria and macrohematuria on November 21, 1989. The patient had been known to have familiar amyloid polyneuropathy for the previous 3 years. Urinalysis showed marked hematopyuria. The residual urine was 216 cc, and urine cultures yielded 10,000,000 colonies of Escherichia coli per ml. Laboratory studies revealed systemic inflammatory findings, but no diabetic change. A plain X-ray film of the abdomen and an excretory urogram (DIP) showed small linear and round gas collections in the bladder shadow. A CT scan revealed multiple gas locules within the bladder wall. A diagnosis of emphysematous cystitis was established. The patient was given antibiotics, and there was striking clinical improvement. Histological examination of the endoscopically obtained biopsy specimen of the bladder revealed amyloidosis. We believe that this patient had a cystitis emphysematosa precipitated neurogenic bladder due to amyloid polyneuropathy and amyloidosis of the bladder.

Aged↗
PubMed1990

Magnetic resonance imaging of Chiari malformations.

The authors describe the features of magnetic resonance (MR) images of Chiari type I and II malformations and present four illustrative cases. Downward displacement of the posterior fossa was more pronounced with type II than type I. A variety of intracranial anomalies were associated with the Chiari type II malformation, whereas type I was mainly associated with syringomyelia and craniovertebral changes. MR imaging is useful in the diagnosis of intracranial anomalies and those situated at the craniovertebral junction, including Chiari malformations.

Adult↗
PubMed1990

[Subependymoma of the septum pellucidum. MRI features and its usefulness in planning surgery].

A 61-year-old female was referred because of headache and gait disturbance. A computed tomography (CT) scan revealed a midline isodense mass lesion. The limit of the tumor was equivocal even after administration of contrast medium. Magnetic resonance imaging (MRI) study, however, clearly showed the relationship between the tumor and the surrounding structures, such as the corpus callosum, the ventricular cavities. The tumor had originated from the region of the septum pellucidum and positioned just beneath the corpus callosum from the genu to the splenium with the roof of the third ventricle pushed downward. The tumor was totally extirpated via the interhemispheric paratranscallosal route. The tumor was typical subependymoma. The post-operative course was uneventful. Transient psychiatric disturbance and inability to retain recent memory were observed and the former subsided in several weeks. CT and MRI characteristics of subependymoma are summarized and the usefulness of MRI to locate tumors and to plan proper surgical approaches are emphasized.

Cerebral Ventricle Neoplasms↗
PubMed1990

[Postoperative facial and vestibular nerve palsy: experimental study of its pathophysiological mechanisms].

The 7th and 8th cranial nerves were shifted in the cerebellopontine (CP) angle of dogs by cerebellar retractions that were similar to those performed in humans with monitoring of auditory evoked brainstem responses (ABR). Postoperatively, the vestibular, facial nerves, and brainstem were histologically examined. Caudal-to-rostral shifts of the nerves could induce vestibular and/or facial nerve damages. The most vulnerable portion of the vestibular nerve was located between the vestibular ganglions and the area vestibularis-the most lateral end of the internal auditory canal. This indicated that due to traction force derived from surgical interventions, the nerves and vessels were avulsed at the fundus of the internal auditory canal. The vestibular nerve may be potentially injured more easily and frequently than the cochlear and facial nerves in retromastoid craniectomies with lateral decubitus position in humans. Direct injuries of the facial nerves in the CP angles were not observed in this study. It was elucidated that the facial nerve was usually injured in the facial canal proximal to the geniculate ganglion due to traction force derived from manipulations in the CP angle. It is likely that as facial nerve edema progresses postoperatively, the facial nerve is gradually compressed within the narrow labyrinthine portion of the facial canal. This may be the cause of delayed postoperative facial nerve palsy. The importance to recognize how not only cochlear but also vestibular and facial nerve are injured by the usual manipulations in the CP angle is stressed.

Animals↗
PubMed1990

[Recordings of evoked electromyographic responses from the extraocular muscles to monitor the oculomotor, trochlear, and abducens nerve function during skull base and orbital surgery].

The methods to record evoked electromyographic responses by micro malleable clip electrodes applied directly to the extraocular muscles are described. This electrophysiological monitoring enables surgeons to localize the ocular motor nerves accurately in the skull base of the middle, posterior fossa, and orbit. In cavernous sinus surgery, electrical stimulation over the dura elicited vigorous responses from the extraocular muscles and subsequently it was possible for the surgeon to avoid severing the ocular motor nerves. In orbital surgery, distended and thinned extraocular muscles were precisely localized and preserved anatomically and functionally. These monitoring methods may play the same role as electrical stimulation to the facial nerves in acoustic neuroma surgery.

Abducens Nerve↗
PubMed1990

[Cyst formation of meningioma: report of two cases and review of literature].

Cystic meningioma is rare and said to account for 1 to 2% of all types of meningioma. Here we report two cases of cystic meningioma. Case 1 is a 25-year-old man. He had suffered from sporadic epileptic seizure for about two years until the first CT was performed. It revealed a large cystic lesion at the right parietal region in touch with the midline structure. A nodular high density area was delineated in the contrast enhanced CT on the medial wall of the cyst. He showed no neurological abnormality at all. Case 2 is a 56-year-old female who had been complaining of memory disturbance, and an episode of sudden dizziness followed by a syncope that lasted for a few minutes. Neurological examination revealed slight right hemiparesis, right homonymous hemianopsia and left papilledema. A large cystic lesion was delineated at the temporal region in the plain CT, and no positively enhanced region could be found in the contrast enhanced CT. After surgical removal of the tumors, both cases were identified as cases of meningioma. They were diagnosed histopathologically as a fibroblastic meningioma and a meningotheliomatous meningioma respectively. The wall of the cyst consisted of the cortical surface per se in both cases. Preoperative diagnosis of such cases and the mechanism by which the cyst is formed were also discussed.

Adult↗
PubMed1990

[A case of arachnoid cyst in the posterior fossa with lower cranial nerve palsy].

We experienced a rare case of arachnoid cyst in the right cerebellomedullary cistern. A 59-year-old female was admitted to our clinic because of lower cranial nerve palsy (deviation of uvula to the left side, swallowing disturbance, curtain sign, hoarseness, atrophy of the right sternocleidomastoid muscle, and deviation of the tongue to the right side). MRI demonstrated a mass lesion compressed towards the medulla oblongata in the right cerebellomedullary cistern. MRI was very helpful as a diagnostic tool, since there is no bone object in the posterior fossa. Although by CT cisternography, the arachnoid cyst was thought to have a communication with the surrounding subarachnoid spaces, the cyst wall was removed because of its compressive symptoms on the lower cranial nerves. After the operation, the cyst shrank, and the clinical symptoms were reduced.

Arachnoid↗
PubMed1989

Common antigenicity of mouse 42 degrees C-specific heat-shock protein with mouse HSP 105.

Mammalian cells incubated at 42 degrees C synthesize a specific heat-shock protein at 42 degrees C (42 degrees C-hsp) that is not induced by heat-shock at 45 degrees C or by other stresses that induce major heat shock proteins (Hatayama et al. (1986) Biochem. Biophys. Res. Commun. 137, 957-963). Antibody raised against a heat-shock protein with molecular weight of 105,000 (hsp 105) purified from mouse FM 3A cells cross-reacted to the 42 degrees C-hsp of the same cells. The antibody reacted only weakly to hsp 105 and 42 degrees C-hsp of human HeLa cells. These results suggested that hsp 105 and 42 degrees C-hsp have the same antigenic determinant, and that 42 degrees C-hsp may have a structure similar to that of hsp 105.

Animals↗
PubMed1989

Cystic cavernous hemangioma in the frontal lobe in an infant--case report.

A 4-month-old infant was hospitalized following a generalized convulsion. Computed tomography showed a lesion of heterogeneous high density and a large low-density area in the right frontal lobe. At surgery, an angiomatous tumor was found within a large cyst containing bloody fluid. Histopathological diagnosis was a cavernous hemangioma. Only eight other cavernous hemangiomas in infants have been reported. The characteristic clinical features and computed tomographic findings of these cases are discussed.

Brain Neoplasms↗
PubMed1989

Responses of pineocytoma to radiation therapy and chemotherapy--report of two cases.

Pineal parenchymal tumors are so rare that their responses to radiation and/or chemotherapy are not well known. Two cases of pineocytoma, which responded well to radiation therapy and chemotherapy, are reported. A 45-year-old female received radiation therapy in a total dose of 5000 rads to the tumor. Three months after the completion of therapy, computed tomography (CT) showed complete disappearance of the tumor, and she remains well as of 1 year after treatment. The second patient, a 6-year-old girl, underwent four cycles of intravenous infusion of ACNU (25 mg) over 10 months. CT demonstrated complete disappearance of the tumor and, 18 months post-operatively, there was no evidence of tumor recurrence. The results in these two cases indicate that postoperative radiation therapy and/or chemotherapy should be strongly considered for patients with pineal parenchymal tumors.

Child↗
PubMed1989

[A case of intracranial osteochondroma: its MR images].

A 15-year-old boy was referred to our department due to left abducens palsy and trigeminal neuralgia. CT scans that had been taken fourteen months before this referral failed to disclose any abnormality. CT scans taken on admission, however, demonstrated an irregular calcified lesion in the left parasellar region. This implied that some ossifying process had been progressing during these fourteen months. MR images clearly demonstrated tumor contour and it was shown that high and low intensity spots were evenly scattered within the tumor. Consequently, tumor appearances in MR images were totally different from those in CT scans. MR images were useful in investigating contour and extension of osteochondroma, but CT scans were necessary to locate calcified foci within the tumor.

Adolescent↗