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Biomedical subjects

T He

Publications and source records attributed to T He.

At least 91 records · Page 5Linked to original sources
PubMed1996

Nutritional analysis and an enriched medium for fermentative treponemes isolated from subgingival plaque.

In order to cultivate fermentative treponemes isolated on Medium 10 with the plate-in-bottle method from subgingival plaque, we developed the enriched Medium 10. Enriched Medium 10 broth consisted mainly of Trypticase peptone (2 g/L), yeast extract (10 g/l), glucose (5 g/l) and volatile fatty acids mixture (3.1 ml/l). The growth curves in enriched Medium 10 broth showed that clinical isolates of Treponema socranskii reached the early stationary phase on the 3rd-5th day, and the doubling times averaged 22.2h. By using enriched Medium 10, the nutritional analysis of oral treponemes was examined for each compound of the volatile fatty acid and various sera. Clinical isolates were found to require iso-butyric acid, 2-methylbutyric acids and/or iso-caproic acid independently for their growth. However, the growth of these fermentative treponemes was inhibited by addition of various sera to this medium.

Animals↗
PubMed1996

Microbial flora in the acute phase of periodontitis and the effect of local administration of minocycline.

Periodontitis, similar to other infectious diseases, is known to progress as chronic inflammation with recurrent acute phases. The purpose of this study was to clarify the microbiological composition of the acute phase and to compare the bacterial flora with that of comparable chronic periodontal pockets. We also evaluated the effect of application of minocycline gel locally on the change in the microflora in the acute pockets. Microbial flora from the subgingival pockets of 28 patients in the acute phase of periodontitis and of 12 patients in a comparable chronic phase as the control were investigated by various bacterial culture methods including TS blood agar and TSBV plates. Minocycline gel was applied to the acute periodontal pockets. Changes in the microbiological proportion and clinical parameters at one week after baseline examination were followed by dark-field analysis, culture method, and indirect immunofluorescence technique. Characteristic features of bacterial proportions in the acute site were observed as an increase in Bacteroides forsythus. The number of Porphyromonas gingivalis and black pigmented anaerobic rods also increased. Application of minocycline gel in the acute pocket without any debridement produced improvement in clinical symptoms at one week. Black-pigmented anaerobic rods, P. gingivalis, and B. forsythus decreased significantly at one week after the application. Results indicate that periodontopathic bacteria including B. forsythus and P. gingivalis were predominant in the acute phase of periodontitis and a locally delivered antibiotic may be effective as an alternative modality of treating the acute inflammation.

Acute Disease↗
PubMed1996

[Evaluation of non radioactive DNA probe (Affirm DP) for detecting periodontopathic bacteria].

The purpose of this investigation was to evaluate the potential of application of non-radioactive DNA probe method (Affirm DP) in clinical diagnosis and evaluation of treatment efficacy. Sensitivity and specificity of two different cut-off levels (10(4), 5 x 10(5)) of the method with bacterial culture in detection of Bacteroides forsythus, Porphyromonas gingivalis, and Actinobacillus actinomycetemcomitans were compared. Those bacteria were obtained from bacterial cultures and clinical samples from healthy subjects and patients with periodontal disease. The results showed that detection percentage of DNA probe method with 10(4) cut-off was equal to or higher than the culture method for Bacteroides forsythus and Porphyromonas gingivalis detection. Therefore, it is suggested to be a useful chairside DNA probe kit as an aid in clinical diagnosis and evaluation of treatment efficacy.

Aggregatibacter actinomycetemcomitans↗
PubMed1996

[Measurement of maximal inspiratory flow and forced inspiratory capacity and its clinical application].

The methods of measuring the maximal inspiratory flow (V(imax)) and the forced inspiratory capacity (FIC) via forced inspiratory capacity-time curve (FIC-t curve) were investigated. Both V(imax) and FIC were measured in 35 normal subjects and 89 patients with chronic obstructive pulmonary disease (COPD). The maximal inspiratory pressure (MIP) was measured simultaneously at function residual capacity(FRC) level by modified Black Method. The results showed that there is a linear relationship between MIP and V(imax) or FIC in both normal subjects and patients with COPD. Normal subjects had a mean V(imax) and FIC much higher than those of patients with COPD. The values of V(imax) and FIC in patients were also, significantly correlated to the severity of COPD. So we suggest that both V(imax) and FIC be used as clinical indices to reflect inspiratory muscles strength.

Adult↗
PubMed1996

[Cetirizine improves the resistance of airway and pulmonary function in patients with asthma].

The objective of this study was to investigate the possible anti-asthma role of Cetirizine. Forty asthmatics were randomly divided into two groups. The experimental group had 30 patients. Among them were 10 patients with simple asthma, 5 patients complicated by mild emphysema, 6 patients complicated by moderate emphysema and 9 patients complicated by severe emphysema. Vit-C (control) group had 10 cases, including 2 cases of simple asthma, 6 cases complicated by mild emphysema and 2 cases complicated by moderate emphysema. All patients had a single oral dose of 5 mg Cetirizine or 0.1 g Vit-C blindly. Before and 0.5, 1 hour after their medicines, the resistance of airway (Raw), sGaw and MEFV were examined in all patients on 6200 Plethysmograph. The measured values showed a significant improvement of Raw and sGaw after administration of Cetirizine. In half an hour after Cetirizine, the Raw decreased by 20.408%, and sGaw increased by 28.249%. In one hour after Cetirizine, Raw further decreased by 24.34% and sGaw increased by 41.153% (P < 0.001). The FVC in MEFV increased by 4.96% (P < 0.02) in one hour after Cetirizine, but other parameters in MEFV curve (PEF, FEV1, MMEF) had no significant changes. All parameters in control group had no significant changes (P > 0.05). The results indicate that Cetirizine could decrease Raw in asthmatics, improve their lung ventilatory function. Cetirizine is a new H-receptor antagonist usually used as anti-inflammatory and allergy suppression medication. It is shown that Cetirizine is a promising anti-asthma agent in treating bronchial asthma.

Adult↗
PubMed1996

[Diagnostic value of exercise diffusion capacity test on diffuse pulmonary fibrosis].

Diffusion capacity for carbon monoxide of the lung (DLCO) before-and-after exercise test and 5 routine pulmonary function tests were conducted in 16 patients with diffuse pulmonary fibrosis (DPF), 19 patients with interstitial pneumonia (IP), 17 patients with COPD, and 22 normal subjects. The data showed: in normal subjects the DLCO after exercise increased significantly compared with before (P < 0.001). But in DPF group the DLCO before exercise was below the normal range, and it went down further after exercise, the decreasing rate being 17.95% (P < 0.001). The DLCO in IP and COPD groups did not significantly change before and after the exercise. Two cases of interstitial pneumonia, who had a reduced DLCO after exercise, were followed up for 0.5-1.0 year, and both patients developed into DPF by that time. The results suggest that the DLCO measure before and after exercise test may have important value for DPF diagnosis, and a reduced DLCO after exercise test in IP patients may warn the development from IP into DPF.

Adult↗
PubMed1996

[Value of measuring resistance of airway for diagnosis of asthma].

Resistance of airway (Raw), sGaw and Maximal expiratory flowvolume curve (MEFV) were measured in 35 normal subject, 43 patients with remission asthma and 100 patients with remission chronic bronchitis (some complicated by obstructive emphysema). The measured values showed that in simple asthma Raw was significantly higher than that in normal subject and in patients with chronic obstructive bronchitis (COB), but MEFV was in normal range. In chronic bronchitis the value of Raw was higher than that in normal subject, but lower than that in asthma (0.05 > P > 0.02); however the FEV1, MMEF, V75 in the curve of MEFV were significantly decreased at the stage of COB, with the most remarkable decrease in MMEF. The results suggest that Raw is a sentitive test for diagnosis of simple asthma, in addition to MEFV measurement; it may be helpful for differential diagnosis of simple asthma and COB and may has potential value for clinical practice.

Adult↗
PubMed1995

Evaluation of changes in the size and location of the prostate, seminal vesicles, bladder, and rectum during a course of external beam radiation therapy.

PURPOSE: To document the size and location of the prostate, seminal vesicles, bladder, and rectum throughout the course of external beam radiotherapy. The frequency and range of motion of these organs are quantified. METHODS AND MATERIALS: Ten patients with localized carcinoma of the prostate had conventional simulation followed immediately by a treatment planning computed tomography scan (TPCT0). Once treatment was initiated, each patient had a weekly CT (TPCT1-N) before or after his daily treatment. Anatomical structures from CT were delineated on a computer workstation for analysis. The serial CT sets were spatially registered to the initial scan using image correlation software that brings into congruence the bony pelvis of the different scans. The location of the prostate, seminal vesicles, bladder, and rectum on subsequent scans were compared to TPCT0, as well as to each other. RESULTS: Prostate volumes were observed to vary by an average of +/- 10% during the course of radiation therapy, while the seminal vesicle volumes varied by as much as 100%. Bladder and rectal volumes varied by +/- 30%. Compared to TPCT0, movement of the prostate was demonstrated in all patients. Quantitation of the center-of-mass (CM) showed motion of less than 1 mm in the left-right direction, while motion ranging from 0 to +/- 1 cm was observed in the anterior-posterior and superior-inferior directions. The individual standard deviations of these motions varied from approximately 1-5 mm. These variations were correlated to changes in the dimensions of the bladder and rectum. CONCLUSIONS: Changes in the location of the prostate, seminal vesicles, and normal tissue volumes during the course of radiation therapy occur and have dosimetric consequences that may impact tumor control and normal tissue complication probabilities. Conformal therapy for prostate cancer will require the incorporation of knowledge of the anatomic relationships of these structures as a function of time. Therefore, these uncertainties must be taken into account when designing treatment plans and in considering dose escalation trials.

Humans↗
PubMed1995

[Measurement of the reserve function of inspiratory muscle and its clinical significance].

The principles of measuring inspiratory muscle tension-time index (TTim) and the ratio of the works of inspiration over the maximal works of inspiration (Wi/Wi(max)) were investigated and their formulae were deduced, i.e. TTim = (Pi x Ti)/(MIP x Ttot) and Wi/Wi(max) = (Pi x VT)/(MIP x IC). The importance of the inspiratory pressure and the maximal inspiratory pressure (MIP) measured at function residual capacity (FRC) level was emphasized. Both TTim and Wi/Wi(max) were measured in 35 normal subjects and 89 patients with chronic obstructive pulmonary disease (COPD). The results showed that normal value of TTim was 0.0253 +/- 0.0055 which corresponds to the normal value of the diaphragm tension-time index (TTdi = 0.02-0.03) reported by Bellemare. Patients with COPD had a mean TTim much higher than that of normals (P < 0.01). The works of inspiration (Wi) in patients with COPD increased, while the maximal works of inspiration (Wi(max)) declined, so Wi/Wi(max) became significantly greater than that of normals (P < 0.01). The results also showed that there was a linear relationship between Pi/Pimax and TTim or Wi/Wimax (r = 0.7891, 0.9738, 0.6459, 0.9327, P < 0.01). Therefore, we suggest that both TTim and Wi/Wimax can be used as clinical indices to reflect the reserve function of inspiratory muscles.

Adult↗
PubMed1994

Comparison of a conformal nonaxial boost with a four-field boost technique in the treatment of adenocarcinoma of the prostate.

PURPOSE: A comparative study of a standard four-field boost technique and a customized nonaxial external beam technique for the treatment of adenocarcinoma of the prostate was performed to quantify rectal and bladder sparing. METHODS AND MATERIALS: Sixteen patients with localized carcinoma of the prostate who underwent simulation and treatment planning computed tomography formed the basis of this study. The prostate, seminal vesicles, lymph node, pelvic bones, rectum, bladder, and skin volumes were contoured from the CT slices to the three-dimensional treatment planning system. Each patient was planned for both standard four-field and nonaxial techniques to a boost treatment dose of 24 Gy to the prostate. Isodose plans were chosen on the basis of adequate tumor coverage and normal tissue sparing. RESULTS: The volumes of the prostate, rectum, and bladder ranged from 46-148 cc, 25-196 cc, and 50-378 cc, respectively. Data analysis demonstrated a statistically significant reduction (p < 0.01) in normal tissue irradiation with the nonaxial external beam technique at dose levels of 15 and 20 Gy. CONCLUSION: The nonaxial four-field external beam technique appears to be superior to the standard four-field approach in reducing the volume of high dose irradiation to the adjacent rectum and bladder.

Adenocarcinoma↗
PubMed1994

Serologic survey of giant pandas (Ailuropoda melanoleuca), and domestic dogs and cats in the Wolong Reserve, China.

Sera from captive and recently rescued giant pandas (Ailuropoda melanoleuca) in the Wolong Reserve, China, were examined by serum neutralization or hemagglutination inhibition for antibodies to canine distemper virus (CDV), canine coronavirus (CCV), canine herpesvirus (CHV), pseudorabies virus (PRV), canine adenovirus type 2 (CAV), and canine parvovirus (CPV). Serum samples from village domestic dogs and cats, which run free throughout the reserve also were examined. Antibodies against CPV were detected in six of eight giant pandas and all dogs and cats tested. The origin of the virus was not determined. Two of eight giant pandas and two of seven dogs had CDV antibody titers. Three of eight pandas and three of seven dogs had CCV antibody titers. Four of eight pandas and two of seven dogs had CAV titers; the titers in dogs were very high. No pandas or dogs had evidence of exposure to CHV or PRV.

Adenoviruses, Canine↗
PubMed1994

[Relationship between airway obstruction and inspiratory muscle function].

Measurement of Pimax (maximal static inspiratory mouth pressure), Pi (static inspiratory mouth pressure) and 5 routine pulmonary function tests, which inspect the degree of airway obstruction, was done in 157 patients with COPD and 55 normal subjects. The results indicated that along with the deterioration of COPD, the Pimax was getting decreased and the Pi/Pimax was getting increased. In the mild and moderate COPD groups, however, the Pi and Pi/Pimax showed no statistical significance, which was the only exception. Our results suggested that (1) the airway obstruction could reduce the inspiratory muscle strength; and (2) the Pi/Pimax might be useful in clinical practice for early diagnosis of inspiratory muscle insufficiency.

Adult↗
PubMed1993

[Specific curative effects of nitrosourea drugs on tumor cells with Mer--phenotype in Chinese].

O6-Methylguanine DNA methyltransferase (O6-MT) activity and cellular sensitivity to nitrosourea drugs of 10 kinds of tumor cell strains derived from Chinese patients were measured by 3H radioactivity and colony-forming ability, respectively. The results in vitro showed that nimustine (Nim) 25 micrograms.ml-1 and carmustine (Car) 20 micrograms.ml-1 exhibited specific killing effects on Mer-phenotype tumor cells characterized by low O6-MT activity. In vivo both Nim and Car (25 mg.kg-1.wk-1 x 4 wk, ip) had specific curative ability to Mer- tumor cells implanted in nude mice. These findings suggested that assay of O6- MT activity in tumor biopsy could be used as a predictable guide to human tumor chemotherapy with nitrosourea compounds.

Animals↗
PubMed1993

[Effects of "he xiang zhuang gongfu" on respiratory function in healthy adults].

Eleven healthy adults who practised He Xiang Zhuang Gongfu (HGF) were followed for 6 months; 15 pulmonary function tests were determined before HGF practice, 2 months and 6 months after HGF practice. All 11 subjects took the HGF exercise every day for 30 minutes. Samplings were taken before subjects had ever practised HGF as control value, immediately and 30 minutes after HGF exercise. In the 2nd month and 6th month of HGF practice. The results indicated that VO2 decreased after 2 and 6 months HGF practice, and the effect even persisted to 30 minutes after HGF exercise, indicating that HGF may reduce metabolic rate and decrease the oxygen consumption of body. The possible mechanism was discussed. MVV had an up tendency after HGF practice, which suggested the possibility of strengthening respiratory muscle by HGF. HGF may be a good physical exercise and can induce a wakeful hypometabolic physiologic state.

Adult↗
PubMed1992

[Pulmonary function disturbances in patients with hepatic cirrhosis].

The results of five pulmonary function tests on 52 patients with uncompensated hepatic cirrhosis were presented. Compared with matched healthy adults, the patients had decreased values of VC, TLC and DLCO, and their RV/TLC increased (P < 0.05-0.001). Possible pathogenic factors were investigated. The ascites showed evident influences on pulmonary function. In addition to the disorders detected by the above 4 tests, FRC decreased in the patients with ascites. A comparison between the patients with albumin below 30g/L and the patients with albumin above 30g/L revealed that reduced plasma albumin also played role in decreasing VC, TLC and DLCO. Besides the effects of ascites, severe varices of esophagus should have relation to the increase of RV/TLC and the decrease of DLCO.

Adult↗